CAJ | 학술논문

目的：比较3种核桃仁提取物对阿尔茨海默病(AD)模型大鼠学习记忆能力及海马区超微病理的影响。方法采用 Meynert 基底核注射 Aβ1-40建立 AD 模型,造模成功后随机分为核桃仁-水组、核桃仁-乙醇组和核桃仁-丙酮组,模型对照组,每组10只。另外选取正常对照组10只和假手术组10只。模型对照组不进行灌胃治疗；核桃仁-丙酮组、核桃仁-乙醇组和核桃仁-水组于造模成功后分别灌胃给予核桃仁丙酮提取物、乙醇提取物、水提取物(相当于生药0.3 g·mL-1),灌胃剂量为3 g·kg-1。以 Morris 水迷宫评价大鼠学习记忆能力,用透射电镜观察神经元超微结构。结果核桃仁-丙酮组、核桃仁-乙醇组、核桃仁-水组在前3 d 寻找平台的时间下降较迅速,第3天分别为(51.80±4.37),(61.20±4.67),(59.63±5.24)s,模型对照组为(67.67±6.12)s,从第3天开始趋于平稳,与模型对照组比较,仍差异有统计学意义(P<0.05),以核桃仁-丙酮组效果最佳(P<0.01),海马区神经元超微结构基本正常。结论核桃仁丙酮提取物具有较好防治 AD 的作用。
목적：비교3충핵도인제취물대아이자해묵병(AD)모형대서학습기억능력급해마구초미병리적영향。방법채용 Meynert 기저핵주사 Aβ1-40건립 AD 모형,조모성공후수궤분위핵도인-수조、핵도인-을순조화핵도인-병동조,모형대조조,매조10지。령외선취정상대조조10지화가수술조10지。모형대조조불진행관위치료；핵도인-병동조、핵도인-을순조화핵도인-수조우조모성공후분별관위급여핵도인병동제취물、을순제취물、수제취물(상당우생약0.3 g·mL-1),관위제량위3 g·kg-1。이 Morris 수미궁평개대서학습기억능력,용투사전경관찰신경원초미결구。결과핵도인-병동조、핵도인-을순조、핵도인-수조재전3 d 심조평태적시간하강교신속,제3천분별위(51.80±4.37),(61.20±4.67),(59.63±5.24)s,모형대조조위(67.67±6.12)s,종제3천개시추우평은,여모형대조조비교,잉차이유통계학의의(P<0.05),이핵도인-병동조효과최가(P<0.01),해마구신경원초미결구기본정상。결론핵도인병동제취물구유교호방치 AD 적작용。
Objective To compare the influence of three kinds of Walnut kernel extracts on learning and memory ability as well as ultrastructural pathology in hippocampus of Alzheimer's disease (AD) rats. Methods AD rat model was established by injection of amyloid-beta protein (Aβ1-40 ) into the nucleus basalis of meynert. The AD rats were randomly divided into Walnut kernel-water group,Walnut kernel-ethanol group,Walnut kernel-acetone group,and the model control group,10 rats in each group. In addition,10 rats of normal control group and 10 rats of sham operation group were selected. The model control group was not treated; the treatment groups were intragastrically given Walnut kernel water extract,ethanol extract,and acetone extract ( the equivalent of pharmacognosy 0. 3 g·mL-1 ),respectively,dose 3 g·kg-1 . The learning and memory ability was studied by Morris water maze,and ultrastructure of neurons was observed under the transmission electron microscopy. Results The time of looking for platform in Walnut kernel-water group,Walnut kernel-ethanol group,and Walnut kernel-acetone group were dropped swiftly at the beginning of 3 days, the third day is (51. 80±4. 37),(61. 20±4. 67),and (59. 63±5. 24),respectively; the model control group is (67. 67±6. 12) s. Compared with the model group,the differences were significance (P<0. 05); However,the acetone extract of Walnut kernel can obviously enhance the learning and memory ability (P<0. 01),and the ultrastructure almost returned to normal. Conclusion The acetone extracts of Walnut kernel have the function of preventing Alzheimer's disease.