CAJ | 학술논문

目的：探讨芡实醇提物对糖尿病肾病大鼠肾功能的影响，并测定芡实醇提物、水提物上清和沉淀的体外抗氧化能力。方法80%乙醇提取芡实种仁，浓缩冻干后得干燥粉末，药渣采用水提醇沉法，经抽滤旋蒸制备上清和沉淀。雄性SD大鼠腹腔注射链脲佐菌素60 mg/kg诱导糖尿病肾病模型，灌胃给予芡实醇提物（75、150、300、600 mg/kg）12周后，检测大鼠尿蛋白、尿微量白蛋白、血肌酐（Cr）和尿素氮（BUN）含量，并观察肾组织病理学变化。通过对Fe3+还原力、1，1-二硝基-2-三硝基苯肼（DPPH）、O2－和OH－自由基清除率的比较，测定3种提取物的抗氧化能力。结果与糖尿病肾病模型组比较，各剂量芡实给药组均能降低大鼠24 h尿蛋白和尿微量白蛋白含量，芡实150和600 mg/kg组尿蛋白显著降低（P＜0.05）；与模型组比较，各剂量芡实给药组大鼠血中BUN均有不同程度降低，芡实300 mg/kg组降低更为显著（P＜0.01）。12周末肾组织病理学观察结果表明，芡实醇提物300和600 mg/kg能够明显减轻肾组织损伤。芡实醇提物在浓度为0.6、0.8和1.0 mg/ml时，显示有一定的抗氧化能力，表现为对Fe3+的还原，对DPPH、O2－和OH－的清除。结论芡实醇提物具有降低糖尿病肾病大鼠尿蛋白的作用，该作用可能与其抗氧化能力有关。
목적：탐토검실순제물대당뇨병신병대서신공능적영향，병측정검실순제물、수제물상청화침정적체외항양화능력。방법80%을순제취검실충인，농축동간후득간조분말，약사채용수제순침법，경추려선증제비상청화침정。웅성SD대서복강주사련뇨좌균소60 mg/kg유도당뇨병신병모형，관위급여검실순제물（75、150、300、600 mg/kg）12주후，검측대서뇨단백、뇨미량백단백、혈기항（Cr）화뇨소담（BUN）함량，병관찰신조직병이학변화。통과대Fe3+환원력、1，1-이초기-2-삼초기분정（DPPH）、O2－화OH－자유기청제솔적비교，측정3충제취물적항양화능력。결과여당뇨병신병모형조비교，각제량검실급약조균능강저대서24 h뇨단백화뇨미량백단백함량，검실150화600 mg/kg조뇨단백현저강저（P＜0.05）；여모형조비교，각제량검실급약조대서혈중BUN균유불동정도강저，검실300 mg/kg조강저경위현저（P＜0.01）。12주말신조직병이학관찰결과표명，검실순제물300화600 mg/kg능구명현감경신조직손상。검실순제물재농도위0.6、0.8화1.0 mg/ml시，현시유일정적항양화능력，표현위대Fe3+적환원，대DPPH、O2－화OH－적청제。결론검실순제물구유강저당뇨병신병대서뇨단백적작용，해작용가능여기항양화능력유관。
Objective To investigate the effect of Euryale ferox seeds ethanol extract on renal funtion of diabetic nephropathy (DN) induced by strepotozotocin (STZ) in rats and the antioxidant ability of ethanol extract,water extract supernatant and precipitation. Methods Ethanol extract of Euryale ferox seeds were prepared by ethanol extraction and followed by evaporation and freeze-dry. The seeds residue was further extracted with water and precipitated with ethanol to obtain water extract supernatant and precipitation. Sprague Dawley male rats were given intraperitoneal injection of STZ at a dose of 60 mg/kg to induce DN. STZ-induced DN rats were administrated by gavage with different dose of Euryale ferox seeds ethanol extract(75, 150, 300 and 600 mg/kg body weight) once daily for 12 weeks. 24 h urine was collected for examining urinary protein and microalbumin every four weeks. Blood serum was also collected for examining of serum creatinine (Cr) and blood urea nitrogen (BUN) level after rats sacrificed at the end of 12 weeks after administration, and the pathological changes of kidney were observed. Antioxidant capacity of the three extracts were determined by Fe3+ reduction, and radical scavenging rate to 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazl(DPPH), O2-and OH-free radicals. Results Compared with model group, the content of 24 h urine protein and urine microalbumin of DN rats were reduced in all Euryale ferox seeds ethanol extract administration groups, and 24 h proteinuria were significantly reduced in the group of 150 and 600 mg/kg administration(P<0.05);BUN level was also reduced in all dose of Euayale ferox seeds ethanol extract administration, and more remarkably reduced in the group of 300mg/kg (P<0.01). 12 weeks after administration, kidney damage amelioration was observed in pathological sections of rats in Euryale ferox seeds ethanol extracts 300 and 600 mg/kg groups. Meanwhile, Euryale ferox seeds ethanol extract showed a certain antioxidant abilities in reducing of Fe3+ and scavenging DPPH, O2- and OH- at the concentrations of 0.6, 0.8 and 1.0 mg/ml. Conclusion Euryale ferox seeds ethanol extract could decrease proteinuria of STZ-induced DN rats,this may be associated with its antioxidant capacity.