CAJ | 학술논문

目的：建立环磷酰胺线性探针体内外回收率最优校正方法。方法以复方氯化钠注射液为灌注液，反向透析校正方法中的增量法与减量法校正线性探针，考察不同灌注流速对环磷酰胺线性探针回收率的影响。结果灌注流速为1.0μl/min时，环磷酰胺回收率大幅提高，增量法与减量法回收率分别由（24.11±1.75）%、（26.19±0.76）%提升至（50.93±0.91）%、（53.31±1.23）%，体内回收率为（26.19±0.76）%。结论环磷酰胺适宜进行微透析技术的取样，本实验校正方法简便有效。
목적：건립배린선알선성탐침체내외회수솔최우교정방법。방법이복방록화납주사액위관주액，반향투석교정방법중적증량법여감량법교정선성탐침，고찰불동관주류속대배린선알선성탐침회수솔적영향。결과관주류속위1.0μl/min시，배린선알회수솔대폭제고，증량법여감량법회수솔분별유（24.11±1.75）%、（26.19±0.76）%제승지（50.93±0.91）%、（53.31±1.23）%，체내회수솔위（26.19±0.76）%。결론배린선알괄의진행미투석기술적취양，본실험교정방법간편유효。
Objective To establish an optimal linear probe recovery correction method for in vivo and in vitro cyclophosphamide. Methods with ringer solution as the perfusion, retrodialysis gain and loss method were used to calibrate the linear probe. The impact of different perfusion flow rates on microdialysis relative recovery for cyclophosphamide was investigated by HPLC-MS/MS. Results When the perfusion was 1.0μl/min, in vitro recovery results were in the range of (24.11±1.75)%to (50.93±0.91)%for the gain method, and(26.19±0.76)% to(53.31±1.23)% for the loss method;recovery results in vivo were(26.19±0.76)%. Conclusion Cyclophosphamide is suitable for microdialysis sampling. This experiment calibration method is simple and effective.