CAJ | 학술논문

目的：探讨旱莲草水提取物对高同型半胱氨酸血症（ Hhcy ）大鼠血脂及血管内膜的影响。方法通过喂饲高L-蛋氨酸饲料，复制HHcy大鼠模型。50只SD大鼠随机分为5组，正常组、模型组、洛伐他汀组（2 mg? kg-1）及旱莲草低、高2个剂量组（5，10 g? kg-1），每日灌胃给药。8周后，生化法测定血清同型半胱氨酸（ Hcy）、总胆固醇（ TC）、三酰甘油（ TG）水平，酶联免疫吸附法检测一氧化氮（ NO）、内皮素（ ET）及血栓素B2（ TXB2）水平，胸主动脉切片HE染色在光镜下观察血管形态学改变。结果与模型组相比，高剂量旱莲草组血清NO水平明显上升，其余各项指标均显著降低，胸主动脉血管形态基本正常。结论旱莲草水提取物能够有效降低Hhcy大鼠血清Hcy水平，具有调节血脂、保护血管内膜的作用。
목적：탐토한련초수제취물대고동형반광안산혈증（ Hhcy ）대서혈지급혈관내막적영향。방법통과위사고L-단안산사료，복제HHcy대서모형。50지SD대서수궤분위5조，정상조、모형조、락벌타정조（2 mg? kg-1）급한련초저、고2개제량조（5，10 g? kg-1），매일관위급약。8주후，생화법측정혈청동형반광안산（ Hcy）、총담고순（ TC）、삼선감유（ TG）수평，매련면역흡부법검측일양화담（ NO）、내피소（ ET）급혈전소B2（ TXB2）수평，흉주동맥절편HE염색재광경하관찰혈관형태학개변。결과여모형조상비，고제량한련초조혈청NO수평명현상승，기여각항지표균현저강저，흉주동맥혈관형태기본정상。결론한련초수제취물능구유효강저Hhcy대서혈청Hcy수평，구유조절혈지、보호혈관내막적작용。
Objective To investigate the effects of the aqueous extracts of Eclipta Prostrata L.( EPL) on plasma lipid levels and the structure of vascular intima in rats with hyperhomocysteinemia ( Hhcy ) . Methods High L -methionine diet was used to construct HHcy rat model.Fifty SD rats were randomly divided into five groups:normal group, model group, lovastatin group (2 mg? kg -1 ), Eclipta Prostrata L.(EPL) low-and high-doses groups (5, 10 g? kg -1 ).The drags were administrated by intragastric administration once a day . After 8 weeks administration , the level of serum homocysteine ( Hcy ) , total cholesterol (TC) and trigly-ceride ( TG) were detected by biochemical analysis.While nitric oxide (NO), endothelin (ET) and thromboxane B2 ( TXB2 ) were measured by enzyme linked immunosorbent assay (ELISA).The histopathological examination of the thoracic aortas were observed microscopically afterhematoxylin -eosin ( HE ) staining. Results Compared with model group , serum levels of Hcy , TG, TC, ET and TXB 2 were significantly decreased ( P<0.05 ) in EPL groups and lovastatin group , while NO levels were significantly increased ( P<0.05) in high dose EPL group.HE sections showed that the shape of thoracic aortas looks as normal .Conclusion The aqueous extract of EPL could effectively reduce serum Hcy and regulate plasma lipid levels , thus it could alleviate vascular damage .