中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2015年
10期
865-868
,共4页
张晶%朱振龙%任常军%代少娟
張晶%硃振龍%任常軍%代少娟
장정%주진룡%임상군%대소연
ω-3多不饱和脂肪酸%脑缺氧缺血%炎症反应%神经行为功能
ω-3多不飽和脂肪痠%腦缺氧缺血%炎癥反應%神經行為功能
ω-3다불포화지방산%뇌결양결혈%염증반응%신경행위공능
ω-3 polyunsaturated fatty acids%hypoxia -ischemia brain damage%inflammatory reaction%neurobehavioral function
目的：探讨ω-3多不饱和脂肪酸对新生鼠缺血缺氧性脑损伤（ HIBD）的保护作用。方法将18只怀孕大鼠随机分为3组，对照组（孕鼠6只，新生鼠26只），假手术组（孕鼠6只，新生鼠24只），干预组（孕鼠6只，新生鼠28只）。干预组，灌胃ω-3多不饱和脂肪酸1 mg? g-1? d-1，从怀孕第2天至分娩后第14天。对照组和假手术组，予等量的0.9％NaCl 溶液。用7 d龄新生鼠制作HIBD模型，用称重法测定脑水肿程度，用Western blot法检测脑组织中诱导型一氧化氮合酶和白细胞介素-6的表达，用改良的Morris水迷宫评估学习与记忆功能。结果干预组，新生鼠脑水肿程度明显轻于对照组（ P＜0.05）；干预组，新生鼠大脑皮层的一氧化氮合酶和白细胞介素-6表达显著高于对照组（P＜0.05）；干预组，新生鼠学习记忆能力明显强于对照组（P＜0.05）。结论ω-3多不饱和脂肪酸可能通过抑制白细胞介素-6、一氧化氮合酶等炎症因子的表达，发挥对新生鼠HIBD的保护作用。
目的：探討ω-3多不飽和脂肪痠對新生鼠缺血缺氧性腦損傷（ HIBD）的保護作用。方法將18隻懷孕大鼠隨機分為3組，對照組（孕鼠6隻，新生鼠26隻），假手術組（孕鼠6隻，新生鼠24隻），榦預組（孕鼠6隻，新生鼠28隻）。榦預組，灌胃ω-3多不飽和脂肪痠1 mg? g-1? d-1，從懷孕第2天至分娩後第14天。對照組和假手術組，予等量的0.9％NaCl 溶液。用7 d齡新生鼠製作HIBD模型，用稱重法測定腦水腫程度，用Western blot法檢測腦組織中誘導型一氧化氮閤酶和白細胞介素-6的錶達，用改良的Morris水迷宮評估學習與記憶功能。結果榦預組，新生鼠腦水腫程度明顯輕于對照組（ P＜0.05）；榦預組，新生鼠大腦皮層的一氧化氮閤酶和白細胞介素-6錶達顯著高于對照組（P＜0.05）；榦預組，新生鼠學習記憶能力明顯彊于對照組（P＜0.05）。結論ω-3多不飽和脂肪痠可能通過抑製白細胞介素-6、一氧化氮閤酶等炎癥因子的錶達，髮揮對新生鼠HIBD的保護作用。
목적：탐토ω-3다불포화지방산대신생서결혈결양성뇌손상（ HIBD）적보호작용。방법장18지부잉대서수궤분위3조，대조조（잉서6지，신생서26지），가수술조（잉서6지，신생서24지），간예조（잉서6지，신생서28지）。간예조，관위ω-3다불포화지방산1 mg? g-1? d-1，종부잉제2천지분면후제14천。대조조화가수술조，여등량적0.9％NaCl 용액。용7 d령신생서제작HIBD모형，용칭중법측정뇌수종정도，용Western blot법검측뇌조직중유도형일양화담합매화백세포개소-6적표체，용개량적Morris수미궁평고학습여기억공능。결과간예조，신생서뇌수종정도명현경우대조조（ P＜0.05）；간예조，신생서대뇌피층적일양화담합매화백세포개소-6표체현저고우대조조（P＜0.05）；간예조，신생서학습기억능력명현강우대조조（P＜0.05）。결론ω-3다불포화지방산가능통과억제백세포개소-6、일양화담합매등염증인자적표체，발휘대신생서HIBD적보호작용。
Objective To investigate the protective effect of ω-3 polyunsaturated fatty acid (ω-3 PUFAs ) on hypoxic ischemic brain damage(HIBD) in neonatal rats.Methods Eighteen pregnant rats were randomly divided into 3 groups, control group (6 pregnant rats, 26 new-born rats), sham operation group (6 pregnant rats, 24 neonatal rats), intervention group ( 6 pregnant rats , 28 newborn rats ) . Intervention group was administration with ω-3PUFAs (1 mg? g-1? d -1 ) by ga-vage , from 2 d after pregnancy to childbirth after 14 d.The rats in con-trol group and sham operation group were given the same amount of 0.9%NaCl.The newborn mice of 7 d old were made into HIBD model . Brain edema was measured using the gravimetric method .The expression of inducible nitric oxide synthase ( iNOS) and interleukin -6 ( iL-6 ) in brain tissue were detected by Western blot .The learning and memory function was assessed by the modified Morris water maze .Results Com-pared with control group , the newborn mice brain edema degree of inter-vention group was more ease , the difference was statistically significant (P<0.05).The expression of iNOS and interleukin -6 in newborn rat cerebral cortex of intervention group was more higher , the difference was statistically significant ( P <0.05 ) .The rats of learning and memory abilityininterventiongroupwasstronger,thedifferencewasstatisticallysignificant(P<0.05).Conclusion ω-3 polyunsaturated fatty acids may inhibit the expression of interleukin -6 and iNOS, and play a protective role in HIBD of newborn rat.
