CAJ | 학술논문

目的：研究人乳腺癌 MCF -7细胞株经吉西他滨处理后 APE／Ref -1 mRNA和蛋白表达的变化。方法以50 nmol? L-1吉西他滨处理乳腺癌MCF-7细胞，在处理后0，3，7，10，13和16天，测定细胞凋亡比例、存活细胞APE／Ref-1基因mRNA及蛋白表达情况。结果吉西他滨50 nmol? L-1干预后第0，3，7，10，13，16天，细胞APE／Ref-1 mRNA及蛋白表达水平均逐渐增加，与干预天数呈正相关，并与凋亡细胞比例的变化趋势一致。 APE／Ref-1表达水平从第10天起明显增加，第16天达到最高（ P ＜0.05）。并且干预至第10，13，16天后，细胞中的APE／Ref-1 mRNA和蛋白表达水平与药物干预前细胞相比有明显差别（ P＜0.05）结论 APE／Ref-1在乳腺癌化疗时表达明显增强，可能与化疗耐药性的产生有关。
목적：연구인유선암 MCF -7세포주경길서타빈처리후 APE／Ref -1 mRNA화단백표체적변화。방법이50 nmol? L-1길서타빈처리유선암MCF-7세포，재처리후0，3，7，10，13화16천，측정세포조망비례、존활세포APE／Ref-1기인mRNA급단백표체정황。결과길서타빈50 nmol? L-1간예후제0，3，7，10，13，16천，세포APE／Ref-1 mRNA급단백표체수평균축점증가，여간예천수정정상관，병여조망세포비례적변화추세일치。 APE／Ref-1표체수평종제10천기명현증가，제16천체도최고（ P ＜0.05）。병차간예지제10，13，16천후，세포중적APE／Ref-1 mRNA화단백표체수평여약물간예전세포상비유명현차별（ P＜0.05）결론 APE／Ref-1재유선암화료시표체명현증강，가능여화료내약성적산생유관。
Objective To investigate the changes of apurinic/apyrimidi-nic endonuclease/redox factor -1 ( APE/Ref -1 ) expression in human breast cancer cell line MCF -7 after induction of gemcitabine and to study the effects of gemcitabine on chemoresistance in breast cancer . Methods After human breast cancer cell line MCF -7 was incubated with 50 nmol? L-1 concentration of gemcitabine for 0,3,7,10,13 and 16 days, the apoptosis ratio was determinated by flow cytometry .And the mRNA and protein levels of APE/Ref -1 of survival cells were deter-mined by reverse transcription -polymerase chain reaction ( RT-PCR) and Western blot , respectively.Results After human breast cancer cell line MCF-7 was incubated with 50 nmol? L -1 gemcitabine for 0,3,7, 10,13 and 16 days,the expression of APE/Ref-1 in human breast can-cer cell line MCF-7 was elevated significantly both at mRNA and pro-tein levels ,and were positively correlated with incubation time , which is consistent with the proportion of apototic cells .And the expression of APE/Ref-1 was increasing significantly from tenthdays , reaching the highest in sixteenth days .And when the drug resistant cells was com-pared with the non -drug resistant cells , which were both incubated with 50 nmol? L-1 gemcitabine for 10,13 and 16 days.The expression of mRNA and protein of APE/Ref-1 in drug resistant cells have statistical significance ( P<0.05 ).Conclusion The obvious up -regulated expression of APE/Ref-1 may be an adaptive re-sponse contributing to the overall chemoresistance in breast cancer .