中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2015年
10期
851-854
,共4页
吴秦川%李三强%胡景峰%付指挥%马曌%韩红梅%杨博%丁康熙%杨省柯
吳秦川%李三彊%鬍景峰%付指揮%馬曌%韓紅梅%楊博%丁康熙%楊省柯
오진천%리삼강%호경봉%부지휘%마조%한홍매%양박%정강희%양성가
二乙基亚硝胺%肝癌%肝糖原%过碘酸雪夫染色
二乙基亞硝胺%肝癌%肝糖原%過碘痠雪伕染色
이을기아초알%간암%간당원%과전산설부염색
N-nitrosodiethylamine%hepatocellular carcinoma%hepatic glycogen%periodic acid-Schiff
目的:探讨在原发性肝癌发展的不同阶段肝糖原的变化规律。方法清洁健康BALB/c雄性小鼠40只,分为正常组( n =3)与模型组( n =37)。将正常组3只小鼠处死,取肝;模型组小鼠每日饲以二乙基亚硝胺( DEN )溶液30μg? mL-1为饮用水,于诱癌4,8,12,16,20,24周,各取3只小鼠进行肝取材( n=21)。将各组肝组织进行糖原过碘酸雪夫氏( PAS)染色。并对样本进行平均光密度分析,用ANOVA显著分析和Student -Newman-Keuls检验。结果正常组和8,12周模型组的糖原含量依次显著降低,16周模型组糖原含量达最低;随后20,24周模型组肝糖原含量依次显著增加。结论 DEN诱导小鼠发生肝癌的过程中,肝糖原的变化规律为先减少后增加。
目的:探討在原髮性肝癌髮展的不同階段肝糖原的變化規律。方法清潔健康BALB/c雄性小鼠40隻,分為正常組( n =3)與模型組( n =37)。將正常組3隻小鼠處死,取肝;模型組小鼠每日飼以二乙基亞硝胺( DEN )溶液30μg? mL-1為飲用水,于誘癌4,8,12,16,20,24週,各取3隻小鼠進行肝取材( n=21)。將各組肝組織進行糖原過碘痠雪伕氏( PAS)染色。併對樣本進行平均光密度分析,用ANOVA顯著分析和Student -Newman-Keuls檢驗。結果正常組和8,12週模型組的糖原含量依次顯著降低,16週模型組糖原含量達最低;隨後20,24週模型組肝糖原含量依次顯著增加。結論 DEN誘導小鼠髮生肝癌的過程中,肝糖原的變化規律為先減少後增加。
목적:탐토재원발성간암발전적불동계단간당원적변화규률。방법청길건강BALB/c웅성소서40지,분위정상조( n =3)여모형조( n =37)。장정상조3지소서처사,취간;모형조소서매일사이이을기아초알( DEN )용액30μg? mL-1위음용수,우유암4,8,12,16,20,24주,각취3지소서진행간취재( n=21)。장각조간조직진행당원과전산설부씨( PAS)염색。병대양본진행평균광밀도분석,용ANOVA현저분석화Student -Newman-Keuls검험。결과정상조화8,12주모형조적당원함량의차현저강저,16주모형조당원함량체최저;수후20,24주모형조간당원함량의차현저증가。결론 DEN유도소서발생간암적과정중,간당원적변화규률위선감소후증가。
Objective To analyse the change of hepatic glycogen in the process of hepatocellular carcinoma development in N -nitrosodiethy-lamine(DEN) -induced mice, and investigate the relationship between the hepatic glycogen content and the different stage of liver cancer . Methods There were forty clean and healthy male mice .They were di-vided in two groups ,the normal group ( n=3 ) and the model group ( n=37).After adapting environment in one week ,the group of normal mice were sacrificed and all the model animals were administrated by solution of DEN (30 μg? mL-1 ) in the carcinoma-induced period.Every three mice were sacrificed in the this group induced by DEN in 4 , 8 , 12 , 16 , 20 ,24 weeks(n=21).The obtained liver tissues divided in seven groups and embedded by paraffin were cut at thick piece .After staining with pe-riodic acid-Schiff ( PAS) the hepatic glycogen were observed .In order to obtain the mean optical density , samples of each group were disposed by the software Motic Images Advanced 3.2.Lastly.Analysis of variance ( ANOVA) test was followed by individual comparison by Student -New-man-Keul test using SPSS V16.0.Result The contents of hepatic gly-cogen in normal group , 8-week and 12-week groups were decreased successively and significantly , and the 16-week were least .The con-tents of hepatic glycogen in 16-week compared with 20-week and 24-week groups were increased successively and significantly . Conclusion In the process of development of hepatic carcinoma induced by DEN , the regular of hepatic glycogen′s change was decreasing gradually at the prior period ,and then the level of hepatic glycogen increased later .