中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2015年
10期
833-835
,共3页
欧维正%骆科文%陈峥宏%陈静%王燕%秦万%蒙俊
歐維正%駱科文%陳崢宏%陳靜%王燕%秦萬%矇俊
구유정%락과문%진쟁굉%진정%왕연%진만%몽준
结核分枝杆菌%利福平%rpoB基因%基因芯片%基因突变
結覈分枝桿菌%利福平%rpoB基因%基因芯片%基因突變
결핵분지간균%리복평%rpoB기인%기인심편%기인돌변
Mycobacterium tuberculosis%rifampicin%rpoB gene%gene chip%gene mutation
目的：了解贵州地区结核分枝杆菌（ MTB）对利福平耐药相关基因rpoB突变特征以便探讨适合本地区MTB对利福平耐药的快速筛选方法。方法用PCR-荧光探针法检测临床标本MTB核酸，阳性者（低浓度除外）用基因芯片检测rpoB基因，异常检测者再进行测序分析。结果 MTB核酸阳性患者3966例，阳性率22.72％（3966／17452）。基因芯片检测2738例中，552例有rpoB 基因突变，突变率为20.16％（552／2738）；9例异常检测者，经基因测序验证，分别为位点缺失、密码子插入、同密码子双碱基突变和多位点突变。 rpoB 基因突变类型有26种，以531，526，516，511，533，513和512位点突变为主，分别占56.23％，18.09％，9.67％，8.61％，4.57％，1.76％和0.18％。结论贵州地区MTB对利福平耐药相关基因rpoB突变特征与文献报道基本一致，基因芯片可作为本地区MTB利福平耐药的快速筛选方法。
目的：瞭解貴州地區結覈分枝桿菌（ MTB）對利福平耐藥相關基因rpoB突變特徵以便探討適閤本地區MTB對利福平耐藥的快速篩選方法。方法用PCR-熒光探針法檢測臨床標本MTB覈痠，暘性者（低濃度除外）用基因芯片檢測rpoB基因，異常檢測者再進行測序分析。結果 MTB覈痠暘性患者3966例，暘性率22.72％（3966／17452）。基因芯片檢測2738例中，552例有rpoB 基因突變，突變率為20.16％（552／2738）；9例異常檢測者，經基因測序驗證，分彆為位點缺失、密碼子插入、同密碼子雙堿基突變和多位點突變。 rpoB 基因突變類型有26種，以531，526，516，511，533，513和512位點突變為主，分彆佔56.23％，18.09％，9.67％，8.61％，4.57％，1.76％和0.18％。結論貴州地區MTB對利福平耐藥相關基因rpoB突變特徵與文獻報道基本一緻，基因芯片可作為本地區MTB利福平耐藥的快速篩選方法。
목적：료해귀주지구결핵분지간균（ MTB）대리복평내약상관기인rpoB돌변특정이편탐토괄합본지구MTB대리복평내약적쾌속사선방법。방법용PCR-형광탐침법검측림상표본MTB핵산，양성자（저농도제외）용기인심편검측rpoB기인，이상검측자재진행측서분석。결과 MTB핵산양성환자3966례，양성솔22.72％（3966／17452）。기인심편검측2738례중，552례유rpoB 기인돌변，돌변솔위20.16％（552／2738）；9례이상검측자，경기인측서험증，분별위위점결실、밀마자삽입、동밀마자쌍감기돌변화다위점돌변。 rpoB 기인돌변류형유26충，이531，526，516，511，533，513화512위점돌변위주，분별점56.23％，18.09％，9.67％，8.61％，4.57％，1.76％화0.18％。결론귀주지구MTB대리복평내약상관기인rpoB돌변특정여문헌보도기본일치，기인심편가작위본지구MTB리복평내약적쾌속사선방법。
Objective To study characteristics of rpoB gene mutations associated with Mycobacterium tuberculosis( MTB) resistance to rifampicin in Guizhou of China to discuss rapid screening methods of detecting MTB resistance to rifampicin.Methods MTB DNA of clinical samples were tested by fluorescent PCR .Low DNA concentration samples were excep-ted.All MTB DNA positive samples were tested by gene chip to screen rpoB gene mutation.The samples tested to be abnormal were further sequenced and aligned. Results MTB DNA of 3966 samples was positive.The positive rate was 22.72%(3966/17452 ).All of the 2738 samples were tested by gene chip , of which 552 samples were detected to have gene mutations and the rate was 20.16%( 552/2738 ) .Nine sam-ples of rpoB gene were tested abnormal .These 9 samples were confirmed to have nucleotide deletion , insertion, the same codon mutation of double base or multisite mutation .There were 26 types of gene mutations and mainly were at site 531 , site 526 , site 516 , site 511 , site 533 , site 513 and site 512 and the mutation rates were 56.23%, 18.09%, 9.67%, 8.61%, 4.57%, 1.76% and 0.18%, separately.Conclusion The characteristics of rpoB gene mutations associated with MTB resistance to rifampicin in Guizhou were basically the same as those in the literature reports.The gene chip can be used as the rapid screening method to detect MTB resistance to rifampicin in Guizhou .
