CAJ | 학술논문

目的：研究姜黄素诱导细胞产生自噬对人乳头瘤病毒（HPV）复制的影响。方法用姜黄素处理人宫颈癌细胞 SiHa 12 h ，荧光酶标仪观察自噬小体形成，免疫印迹试验检测 SiHa 细胞自噬标志蛋白 LC3‐Ⅱ／LC3‐Ⅰ的表达水平；实时定量聚合酶链反应和免疫印迹试验分别检测 HPV E6基因 mRNA 表达和蛋白水平。加入自噬抑制剂3‐甲基腺嘌呤（3‐MA），实时定量聚合酶链反应和免疫印迹试验分别检测自噬抑制后 HPV E6基因表达情况。结果姜黄素诱导后，与对照组相比，SiHa 细胞中自噬小体的荧光强度明显增高，LC3‐Ⅱ／LC3‐Ⅰ比值明显上升（P＜0．05）；细胞诱导自噬后 HPV E6 mRNA 和蛋白水平明显降低，而加入自噬抑制剂3‐MA 后，HPV E6 mRNA 和蛋白水平均明显增高。结论姜黄素可能通过诱导细胞自噬抑制 HPV 的复制。
목적：연구강황소유도세포산생자서대인유두류병독（HPV）복제적영향。방법용강황소처리인궁경암세포 SiHa 12 h ，형광매표의관찰자서소체형성，면역인적시험검측 SiHa 세포자서표지단백 LC3‐Ⅱ／LC3‐Ⅰ적표체수평；실시정량취합매련반응화면역인적시험분별검측 HPV E6기인 mRNA 표체화단백수평。가입자서억제제3‐갑기선표령（3‐MA），실시정량취합매련반응화면역인적시험분별검측자서억제후 HPV E6기인표체정황。결과강황소유도후，여대조조상비，SiHa 세포중자서소체적형광강도명현증고，LC3‐Ⅱ／LC3‐Ⅰ비치명현상승（P＜0．05）；세포유도자서후 HPV E6 mRNA 화단백수평명현강저，이가입자서억제제3‐MA 후，HPV E6 mRNA 화단백수평균명현증고。결론강황소가능통과유도세포자서억제 HPV 적복제。
Objective To explore the effect of autophagy induced by Curcumin on human papilloma virus (HPV) replication . Methods Human cervical cancer cell SiHa were treated with Curcumin to induce autophagy .Fluorescence microscope was used to observe the autophagosome .Western blotting was performed to analyze the expression level of autophagy marker protein LC3‐ Ⅱ /LC3‐ Ⅰ .Real time‐polymerase chain reaction(PCR) and Western blotting were used to detect HPV E6 mRNA and protein expres‐sion level .After adding the autophagy inhibitor 3‐methyl adenine (3‐MA) ,real time‐PCR and Western blotting was employed to detect the expression level of HPV E6 .Results The fluorescence intensity of SiHa cells treated by Curcumin was significant in‐creased ,while the ratio of LC3‐ Ⅱ and LC3‐ Ⅰ was also significantly increased (P< 0 .05) .The expression of HPV E6 mRNA and protein were decreased significantly after induction of autophagy ,while increased significantly after adding autophagy inhibitor 3‐MA .Conclusion Curcumin might inhibit HPV replication by inducing cell autophagy .