CAJ | 학술논문

目的：研制一种灵敏、稳定的酶联免疫吸附试验底物并进行方法学评价。方法制备了以四甲基联苯胺和过氧化氢脲为主要成分的底物体系，并对其灵敏度、精密性和存放稳定性进行了方法学鉴定试验，且与其他3种底物进行了比较。结果本研究自制底物与3种商品化底物相比，灵敏度高于其中2种，精密性试验平均变异系数为3．50％，4℃存放自制底物第1天、7天、1月、6月、12月测定其酶联物反应所得吸光度值分别为2．268、2．403、2．358、2．278、2．330，标准差为0．056185，变异系数为2．40％。结论本研究自制底物灵敏度和精密度高、稳定性强，配制方法简便、重复性好。既能满足实验室科研需要，作为检验试剂盒的一个重要组分也达到了研发商品化试剂盒的要求，为科研实验及科研转化提供了一种经济、有效的选择。
목적：연제일충령민、은정적매련면역흡부시험저물병진행방법학평개。방법제비료이사갑기련분알화과양화경뇨위주요성분적저물체계，병대기령민도、정밀성화존방은정성진행료방법학감정시험，차여기타3충저물진행료비교。결과본연구자제저물여3충상품화저물상비，령민도고우기중2충，정밀성시험평균변이계수위3．50％，4℃존방자제저물제1천、7천、1월、6월、12월측정기매련물반응소득흡광도치분별위2．268、2．403、2．358、2．278、2．330，표준차위0．056185，변이계수위2．40％。결론본연구자제저물령민도화정밀도고、은정성강，배제방법간편、중복성호。기능만족실험실과연수요，작위검험시제합적일개중요조분야체도료연발상품화시제합적요구，위과연실험급과연전화제공료일충경제、유효적선택。
Objective To develop a sensitive and stable substrate for enzyme‐linked immunosorbent assay(ELISA)and carry out methodology evaluation tests on it .Methods A kind of substrate with tetramethyl benzidine and urea peroxide as its main compo‐nents were made up ,its sensitivity ,precision and storage stability were evaluated and compared with other three kinds of substrate . Results This substrate was more sensitive than two of the three commercial substrates .The variable coefficient of precision test was 3 .5% .The optical densities(OD) of the new substrate after stored one day ,seven days ,one month ,six months ,twelve months in 4 ℃ environment were 2 .268 、2 .403 、2 .358 、2 .278 、2 .330 respectively ,the standard deviation was 0 .056 185 ,the variable coeffi‐cient was 2 .4% .Conclusion The substrate proposed in this paper displays good sensitivity ,precision and stability .The preparation method is simple ,reproducible .This substrate not only satisfies the requirements of laboratory research ,but also meets with the de‐mands for commercial kit development due to its function as an important component of test kits .It is an economical and effective choice for both laboratories and research transformation .