CAJ | 학술논문

目的：比较胶体金免疫层析法和酶联免疫吸附试验（ELISA ）检测血清抗环瓜氨酸肽（CCP）抗体结果差异性。方法采用胶体金免疫层析法和 ELISA 法同步检测110例 RA 患者和110例健康体检者的抗 CCP 抗体。结果2种方法经配对χ2检验，差异有统计学意义（χ2＝174．354，P＜0．01）；K ap pa值评价（K ＝0．890，P ＜0．01）显示2种检测方法具有高度一致性。 ELISA 法灵敏度和特异度分别为83．64％和92．73％，均优于胶体金免疫层析法。结论2种方法检测血清抗 CCP 抗体存在差异，ELISA 法的灵敏度和特异度均较高，优于胶体金免疫层析法。
목적：비교효체금면역층석법화매련면역흡부시험（ELISA ）검측혈청항배과안산태（CCP）항체결과차이성。방법채용효체금면역층석법화 ELISA 법동보검측110례 RA 환자화110례건강체검자적항 CCP 항체。결과2충방법경배대χ2검험，차이유통계학의의（χ2＝174．354，P＜0．01）；K ap pa치평개（K ＝0．890，P ＜0．01）현시2충검측방법구유고도일치성。 ELISA 법령민도화특이도분별위83．64％화92．73％，균우우효체금면역층석법。결론2충방법검측혈청항 CCP 항체존재차이，ELISA 법적령민도화특이도균교고，우우효체금면역층석법。
Objective To evaluate the diagnostic values of enzyme‐linked immunosorbent assay (ELISA ) and colloidal gold im‐munochromatographic assay in detecting serum anti‐cyclic citrullinated peptide(anti‐CCP) antibody .Methods Colloidal gold immu‐nochromatographic assay and ELISA were used to detect serum anti‐CCP antibody in 110 RA patients and 110 healthy cases . Results The data were analysed with the paired Chi‐Square Test (χ2 = 174 .354 ,P < 0 .01) ,the difference was significant .The K ap pa value was 0 .890(P< 0 .01) ,indicating that the coincidence between the two kinds of methods was good .The sensitivity , speciality of ELISA were 83 .64% and 92 .73% respectively .Conclusion In detecting serum anti‐CCP Antibody ,there was dife‐rence between colloidal gold immunochromatographic assay and ELISA according to statistics analysis .The sensitivity and speciality of ELISA were higher than those of colloidal gold immunochromatographic assay .