CAJ | 학술논문

目的：：探讨活性维生素D3对阿霉素诱导的足细胞上皮-间充质细胞转分化( EMT)的干预作用。方法：以体外培养的小鼠足细胞系为研究对象,分六组：正常组( C组)、阿霉素组(即模型组,ADR组)、缬沙坦组( ARB组)、低剂量活性维生素D3组( LVD组)、高剂量活性维生素D3组( HVD组)、缬沙坦+高剂量活性维生素D3组( AHVD组)。干预24 h、48 h后采用RT-PCR检测synaptopodin、P-cadherin、desmin、FSP-1 mRNA的表达。 Western Bolt检测synaptopodin、P-cadherin、desmin蛋白的表达。结果：与C组相比,24 h及48 h ADR组synaptopodin、P-cadherin mRNA及蛋白表达均下降(P<0.01)、desmin mRNA及蛋白表达上调(P<0.01)和FSP-1 mRNA表达上调(P<0.01)。与ADR组相比,24 h时ARB组、LVD组、HVD组、AHVD组synaptopodin mRNA表达均升高(P<0.01)但四组之间差异无统计学意义。48 h时与ADR组相比,LVD组及HVD组synaptopodin mRNA及蛋白表达均升高(P<0.05)。48 h四个药物干预组P-cadherin mRNA及蛋白表达均升高(P<0.05)。24 h及48 h四个药物干预组desmin mRNA及蛋白表达均下调(P<0.01),FSP-1 mRNA表达均下调(P<0.05),但四组之间差异无统计学意义。结论：阿霉素可诱导小鼠足细胞发生EMT,活性维生素D3可通过修复足细胞synap-topodin、P-cadherin及抑制desmin、FSP-1的表达来拮抗足细胞EMT而起到保护足细胞的作用。缬沙坦和活性维生素D3在抑制足细胞EMT方面无协同作用。
목적：：탐토활성유생소D3대아매소유도적족세포상피-간충질세포전분화( EMT)적간예작용。방법：이체외배양적소서족세포계위연구대상,분륙조：정상조( C조)、아매소조(즉모형조,ADR조)、힐사탄조( ARB조)、저제량활성유생소D3조( LVD조)、고제량활성유생소D3조( HVD조)、힐사탄+고제량활성유생소D3조( AHVD조)。간예24 h、48 h후채용RT-PCR검측synaptopodin、P-cadherin、desmin、FSP-1 mRNA적표체。 Western Bolt검측synaptopodin、P-cadherin、desmin단백적표체。결과：여C조상비,24 h급48 h ADR조synaptopodin、P-cadherin mRNA급단백표체균하강(P<0.01)、desmin mRNA급단백표체상조(P<0.01)화FSP-1 mRNA표체상조(P<0.01)。여ADR조상비,24 h시ARB조、LVD조、HVD조、AHVD조synaptopodin mRNA표체균승고(P<0.01)단사조지간차이무통계학의의。48 h시여ADR조상비,LVD조급HVD조synaptopodin mRNA급단백표체균승고(P<0.05)。48 h사개약물간예조P-cadherin mRNA급단백표체균승고(P<0.05)。24 h급48 h사개약물간예조desmin mRNA급단백표체균하조(P<0.01),FSP-1 mRNA표체균하조(P<0.05),단사조지간차이무통계학의의。결론：아매소가유도소서족세포발생EMT,활성유생소D3가통과수복족세포synap-topodin、P-cadherin급억제desmin、FSP-1적표체래길항족세포EMT이기도보호족세포적작용。힐사탄화활성유생소D3재억제족세포EMT방면무협동작용。
Objective:To explore the effect of 1α, 25-dihydroxyvitamin D3 on αdriamycin-induced epithelial-mesen-chymal transition ( EMT) of podocytes. Methods:Mouse podocytes cultured in vitro under different differentiation conditions for 10 days were divided into 6 groups - the control group ( C group) , the experimental group ( ADR group) , the low dose 1α, 25-di-hydroxyvitamin D3 group ( LVD group) , the high dose 1α, 25-dihydroxyvitamin D3 group ( HVD group) , the valsartan group ( ARB group) and the combined group ( AHVD group:high dose 1α, 25-dihydroxyvitamin D3 combines and valsartan) . Each group was continuously intervened with drugs for 24 hours and 48 hours. The expression of protein molecules which were related to EMT such as synaptopodin, P-cadherin, desmin and FSP-1 were detected through reverse transcription polymerase chain reaction ( RT-PCR) . In addition, the expression of protein molecules such as synaptopodin, P-cadherin and desmin were also detected by Western Blot-ting. Results:Compared with the control group, after continuously intervened with drugs for 24 hours and 48 hours, in the ADR group, the expression of synaptopodin mRNA, protein, P-cadherin mRNA, and protein decreased, while the expression of desmin, mRNA, protein, and FSP-1 mRNA increased (P<0. 01). Compared with the ADR group, the expression of synaptopodin and mR-NA increased significantly in the ARB group, the LVD group, the HVD group and the AHVD group (P<0. 01). There was no signif-icant difference among the four drug intervention groups. In addition, the expression of synaptopodin, mRNA, and protein increased (P<0. 05) in the LVD group and the HVD group compared to the ADR group in 48 h. Compared with the ADR group, the expres-sion of P-cadherin and mRNA increased (P<0. 05) in the four drug invention groups. The expression of desmin mRNA and protein and FSP-1 mRNA significantly decreased (P<0. 01) in the four drug invention groups. Furthermore there was no significant differ-ence among the four groups. Conclusion:Adriamycin can induce podocytes injury through EMT. In vitro, 1α, 25-dihydroxyvitamin D3 can antagonize podocyte injury by repairing synaptopodin, P-cadherin expression while inhibiting desmin and FSP-1 expression. It shows that 1α, 25-dihydroxyvitamin D3 can protect podocytes through inhibiting EMT in podocytes. Valsartan and high dose 1α, 25-dihydroxyvitamin D3 have no synergy effect on the inhibition of podocyte EMT.