CAJ | 학술논문

目的：比较碘‐淀粉法、Bernfeld 法和 EPS‐G7速率法3种方法检测酸刺激前后唾液淀粉酶（sAA）活性及其活性比值的差异，探讨3种方法检测 sAA 活性之间的相关性。方法采集5例健康志愿者柠檬酸刺激前后的唾液标本各5份，分别用3种方法3次重复检测每份唾液标本的 sAA 活性，计算 sAA 活性及其活性比值的变异系数，并对3种方法检测的 sAA 活性的相关性进行分析。结果3种方法检测的 sAA 活性及其总变异系数差异有统计学意义（P ＜0．05），其中以 EPS‐G7速率法检测 sAA活性和总变异系数最小；3种方法检测的 sAA 活性比值及其比值的变异系数差异无统计学意义（P＞0．05）；3种方法中的任意2种方法检测的 sAA 活性都有显著相关（P＜0．05），相关系数都在0．96以上。结论3种方法检测的 sAA 活性可以通过回归方程进行相互转换，检测精度以 EPS‐G7速率法的最高，而且 sAA 活性比值的数据处理方式可以减少3种方法之间变异系数的差异。
목적：비교전‐정분법、Bernfeld 법화 EPS‐G7속솔법3충방법검측산자격전후타액정분매（sAA）활성급기활성비치적차이，탐토3충방법검측 sAA 활성지간적상관성。방법채집5례건강지원자저몽산자격전후적타액표본각5빈，분별용3충방법3차중복검측매빈타액표본적 sAA 활성，계산 sAA 활성급기활성비치적변이계수，병대3충방법검측적 sAA 활성적상관성진행분석。결과3충방법검측적 sAA 활성급기총변이계수차이유통계학의의（P ＜0．05），기중이 EPS‐G7속솔법검측 sAA활성화총변이계수최소；3충방법검측적 sAA 활성비치급기비치적변이계수차이무통계학의의（P＞0．05）；3충방법중적임의2충방법검측적 sAA 활성도유현저상관（P＜0．05），상관계수도재0．96이상。결론3충방법검측적 sAA 활성가이통과회귀방정진행상호전환，검측정도이 EPS‐G7속솔법적최고，이차 sAA 활성비치적수거처리방식가이감소3충방법지간변이계수적차이。
Objective To compare the differences of salivary alpha(α) amylase (sAA) activity and its activity ratio from the sali‐va before and after citric acid stimulation and approach the correlations among sAA activity determined by the methods of iodine‐starch ,Bernfeld and EPS‐G7 velocity respectively .Methods Ten saliva samples were collected from five healthy volunteers before and after citric acid stimulation .Their activities were determined three times by the three methods ,and the variation coefficient (CV) of sAA activity and activity ratio were calculated .Moreover ,correlation among sAA activities determined by the three meth‐ods were analyzed .Results The significant differences (P< 0 .05) were found in sAA activity and total CV from three determined methods ,and sAA activity and total CV by the method of EPS‐G7 velocity were minimum .There were no significant differences (P> 0 .05) in sAA activities ratio and its CV ;Significant correlation was found between sAA activity determined by random two of three methods(P < 0 .05) ,and their correlation coefficients were above 0 .96 .Conclusion The sAA activity data determined by three methods could be transformed each other by regression equation ,and determined precision by the method of EPS‐G7 velocity is highest ,and data processing method of sAA activity ratio could decrease differences among CV from three methods .