CAJ | 학술논문

目的：初步探讨 CCR2在 NASH 患者中的表达及意义。方法收集 NASH 患者肝穿标本15例，健康对照肝组织标本5例，进行 HE 染色及 NAS 评分、免疫组化染色、激光共聚焦染色等，检测巨噬细胞、CCR2的表达情况并分析与疾病严重程度的相关性。结果 NASH 患者肝组织中巨噬细胞表达较健康对照组明显增加，并与 NAS 评分呈正相关。NASH 患者肝组织中 CCR2表达较健康对照组明显增加，并且主要表达在肝内巨噬细胞表面。结论 CCR2可能通过肝内巨噬细胞参与 NASH 的发展。
목적：초보탐토 CCR2재 NASH 환자중적표체급의의。방법수집 NASH 환자간천표본15례，건강대조간조직표본5례，진행 HE 염색급 NAS 평분、면역조화염색、격광공취초염색등，검측거서세포、CCR2적표체정황병분석여질병엄중정도적상관성。결과 NASH 환자간조직중거서세포표체교건강대조조명현증가，병여 NAS 평분정정상관。NASH 환자간조직중 CCR2표체교건강대조조명현증가，병차주요표체재간내거서세포표면。결론 CCR2가능통과간내거서세포삼여 NASH 적발전。
Objective To investigate the expression and significance of CCR2 in patients with NASH preliminarily. Methods Fifty specimens of liver tissue from patients with NASH and 5 from healthy controls were collected.The tissues were stained with H&E to estimate the NAFLD activity score (NAS).Detection of macrophages and expression of CCR2 were assessed by immunohistochemistry to analyze the correlation with the degrees of NASH.Moreover,the distribution of macrophage and CCR2 were observed by confocal laser scanning.Results The expression of macrophages was significantly increased in NASH patients than that in healthy controls,which positively correlated with NAFLD activity score.NASH patients also had significantly higher CCR2 levels than the controls,which mainly expressed on the surface of hepatic macrophages.Conclusion CCR2 probably promotes NASH progression through macrophages.