CAJ | 학술논문

目的：：检测糖皮质激素受体基因多态位点( N363S、ER22/23EK、BclI、9β),明确其与大疱性类天疱疮糖皮质激素(以下简称激素)抵抗的相关性。方法：提取激素敏感组(40例)、激素抵抗组(48例)及健康对照组(42名)外周血 DNA,应用 SNaPshotSNP 分型技术进行 GR 基因多态性位点( N363S、ER22/23EK、BclI、9β)检测。结果：激素敏感组、激素抵抗组和健康对照组均检出BclI有3种基因型CC、CG、GG,差异无统计学意义(P>0.05)；N363S、ER22/23EK、9β基因位点不存在多态性。结论： GR基因N363S、ER22/23EK、BclI、9β多态性与BP激素抵抗可能无相关性。
목적：：검측당피질격소수체기인다태위점( N363S、ER22/23EK、BclI、9β),명학기여대포성류천포창당피질격소(이하간칭격소)저항적상관성。방법：제취격소민감조(40례)、격소저항조(48례)급건강대조조(42명)외주혈 DNA,응용 SNaPshotSNP 분형기술진행 GR 기인다태성위점( N363S、ER22/23EK、BclI、9β)검측。결과：격소민감조、격소저항조화건강대조조균검출BclI유3충기인형CC、CG、GG,차이무통계학의의(P>0.05)；N363S、ER22/23EK、9β기인위점불존재다태성。결론： GR기인N363S、ER22/23EK、BclI、9β다태성여BP격소저항가능무상관성。
Objective:To detect the genotype frequency of polymorphic loci( N363S, ER22/23EK, BclI, 9β) of glucocorticoid receptor ( GR ) gene to determine the correlation between glucocorticoid receptor gene polymorphism and glucocorticoid resistance in the patients with bullous pemphigus ( BP ) . Methods:Genomic DNA was extracted from the peripheral blood of the patients in the glucocorticoid sensitivity group ( 40 pa-tients) , glucocorticoid resistance group ( 48 patients) and 42 healthy controls. The mutations in polymorphic loci were detected by SNaPshotSNP. Results:Three kinds of genotypes (CC, CG and GG) of BclI were iden-tified in the three groups, with no significant difference among them ( P>0.05) . There was no polymorphism of N363S, ER22 / 23EK and 9β in the three groups. Conclusion: The polymorphism of GR gene may not be correlated with the glucocorticoid resistance in the patients with BP .