CAJ | 학술논문

目的：探讨环孢素A对大鼠脑缺血再灌注损伤的保护及急性期后的神经功能改善作用。方法250~280g成年雄性SD大鼠52只，随机分为假手术组（A组，n=6），PBS对照组（B组，n=23），10mg/kg环孢素A干预组（C组，n=23）。用线栓法建立大鼠脑缺血再灌注模型，术后C组每天给予环孢素A10mg/kg皮下注射，B组注射等量的PBS溶液。分别于模型建立后第3、7、14、21、30天采用改良神经功能缺损评分（modified neurologi?cal severity scores,mNss）评价神经功能缺损程度，并在第3天取脑组织进行三苯基氯化四氮唑（Triphenyltetrazoli?um Chloride，TTC）染色计算脑梗死体积，第3、30天取脑组织冰冻切片后用免疫荧光检测梗死灶周围活化的小胶质细胞数量，缺血周围区神经元数量，第30天检测梗死灶周围神经胶质细胞的数量，并比较各组间的差异。结果 C组再灌注后第3天（P=0.003）、第7天（P=0.011）、第14天（P=0.000）、第21天（P=0.003）、第30天（P=0.004）的改良神经功能缺损评分均低于B组评分；第3天的脑梗死体积低于B组的体积（P＜0.001）；第3天（P<0.001）、30天（P=0.017）梗死灶周围神经元存活数量均多于B组的数量；第3天（P＜0.001）、30天（P＜0.001）梗死灶周围活化的小胶质细胞数量明显少于B组的数量；第30天梗死灶周围的星形胶质细胞数少于B组的数量（P=0.024）。结论环孢素A能促进大鼠脑缺血再灌注急性期后的神经功能恢复，机制可能是抑制梗死灶周围活化的小胶质细胞及星形胶质的过度增生。
목적：탐토배포소A대대서뇌결혈재관주손상적보호급급성기후적신경공능개선작용。방법250~280g성년웅성SD대서52지，수궤분위가수술조（A조，n=6），PBS대조조（B조，n=23），10mg/kg배포소A간예조（C조，n=23）。용선전법건립대서뇌결혈재관주모형，술후C조매천급여배포소A10mg/kg피하주사，B조주사등량적PBS용액。분별우모형건립후제3、7、14、21、30천채용개량신경공능결손평분（modified neurologi?cal severity scores,mNss）평개신경공능결손정도，병재제3천취뇌조직진행삼분기록화사담서（Triphenyltetrazoli?um Chloride，TTC）염색계산뇌경사체적，제3、30천취뇌조직빙동절편후용면역형광검측경사조주위활화적소효질세포수량，결혈주위구신경원수량，제30천검측경사조주위신경효질세포적수량，병비교각조간적차이。결과 C조재관주후제3천（P=0.003）、제7천（P=0.011）、제14천（P=0.000）、제21천（P=0.003）、제30천（P=0.004）적개량신경공능결손평분균저우B조평분；제3천적뇌경사체적저우B조적체적（P＜0.001）；제3천（P<0.001）、30천（P=0.017）경사조주위신경원존활수량균다우B조적수량；제3천（P＜0.001）、30천（P＜0.001）경사조주위활화적소효질세포수량명현소우B조적수량；제30천경사조주위적성형효질세포수소우B조적수량（P=0.024）。결론배포소A능촉진대서뇌결혈재관주급성기후적신경공능회복，궤제가능시억제경사조주위활화적소효질세포급성형효질적과도증생。
Objective To explore the neuroprotective effect of cyclosporine A against cerebral ischemia in a rat model of cerebral ischemia reperfusion. Methods Fifty-two adult male SD rats, weighted 250-280 gram, were randomly divided into three groups: the sham group (group A, n=6), PBS control group (group B, n=23) and cyclosporine A group (group C, n=23). Group C received hypodermic injection of cyclosporine A 10mg/kg daily after surgery and group B re?ceived equal volume of PBS instead. Modified Neurological Severity(mNss)scores were used to assess the neurological deficits at 3, 7, 14, 21 and 30 days following cerebral ischemia. The infarct volume were measured 3 days after reperfu?sion. The neurons, reactive microglia and astrocytes around the infract area were detected by immunofluorescence at 3 and 30 days after surgery. Results Modified Neurological Severity scores were significantly lower in group C than group B at the third(P=0.003),seventh (P=0.011),Fourteenth (P=0.000),twenty-first (P=0.003) and thirtieth (P=0.004) days after surgery. cyclosporine A reduced infarct volume, reactive microglia and astrocytes while increased survived neurons (P<0.001) in ischemic penumbra 3 and 30 days after reperfusion (all P<0.001). Conclusion Continuous injection of cyclosporine A not only protects neurons against ischemia damage but also improves neurological functional recovery af?ter acute stage of damage, possibly through reduction of reactive microglia cells and proliferation of astrocytes.