CAJ | 학술논문

目的：活体观察脑血管CO2反应性及初步探讨其反应机制。方法利用双光子成像技术，在磨薄的颅窗下观察5只雄性C57小鼠脑皮层动脉、穿支动脉、皮层静脉及毛细血管共4种脑血管对吸入CO2的反应，分别使用一氧化氮合酶抑制剂L-NAME及前列腺素合成抑制剂吲哚美辛阻断血管扩张通路，观察血管反应性变化。结果吸入1 min 5% CO2后4种脑血管分别出现不同程度的扩张，其中穿支动脉扩张最显著（45.01%±4.45%）；使用L-NAME后4种脑血管对CO2反应性较使用前显著下降（P<0.05）；使用吲哚美辛后动脉CO2反应性较使用前及使用L-NAME后显著下降（P<0.05），但毛细血管CO2反应性较使用前无下降（P>0.05）。结论4种脑血管在吸入CO2后出现不同程度的扩张反应，前列腺素类及NO参与介导反应。本实验方法有较好的应用价值。
목적：활체관찰뇌혈관CO2반응성급초보탐토기반응궤제。방법이용쌍광자성상기술，재마박적로창하관찰5지웅성C57소서뇌피층동맥、천지동맥、피층정맥급모세혈관공4충뇌혈관대흡입CO2적반응，분별사용일양화담합매억제제L-NAME급전렬선소합성억제제신타미신조단혈관확장통로，관찰혈관반응성변화。결과흡입1 min 5% CO2후4충뇌혈관분별출현불동정도적확장，기중천지동맥확장최현저（45.01%±4.45%）；사용L-NAME후4충뇌혈관대CO2반응성교사용전현저하강（P<0.05）；사용신타미신후동맥CO2반응성교사용전급사용L-NAME후현저하강（P<0.05），단모세혈관CO2반응성교사용전무하강（P>0.05）。결론4충뇌혈관재흡입CO2후출현불동정도적확장반응，전렬선소류급NO삼여개도반응。본실험방법유교호적응용개치。
Objective To examine cerebrovascular reactivity to CO2 inhalation in mice. Methods In vivo Two-Pho?ton imaging technique was used to record the reaction of cerebral cortical vessels including penetrating artery, surface vein and capillary in 5 male C57 mice after CO2 inhalation under a thinned-skull cranial window. Nitric oxide syntheses inhibitor L-NAME and Prostaglandin syntheses inhibitor Indomethacin were used to block different vasodilator pathways, respectively. Results Different mouse cortical vessels displayed different degrees of dilation to 1-minute 5%CO2 inhala?tion. The penetrating artery exhibited the most obvious dilation (45.01%±4.45%). L-NAME intervention significantly di?minished cerebravascular CO2 reactivity(P<0.05). Indomethacin significantly attenuated the dilation of artery but not capillary comparing with L-NAME intervention(P<0.05). Conclusions Different vessels react differently to CO2 inhala?tion in which postaglandins and NO signal pathways are involved.