目的 测定流行性乙型脑炎(简称乙脑)患者脑脊液中S100B蛋白的含量,探究S100B蛋白在乙脑发病过程中的意义.方法 选取2013年8-10月在河北医科大学第二医院神经内科住院、经河北省疾病控制中心检测乙脑病毒IgM抗体阳性、确诊为乙脑的45例患者,应用ELISA法测量其脑脊液中S100B蛋白浓度.其中男23例,女22例,年龄14 ～72岁,平均(38.8±14.9)岁.按照发病时间分为初期、极期、恢复期,按临床症状的轻重分为轻型、中型、重型,按影像学资料分为MRI未显示病灶组、MRI显示病灶组;选取同期结合临床表现及相关检查除外中枢神经系统感染性疾病的患者12例作为对照组,其中男4例,女8例,年龄18～67岁,平均(37.1±14.5)岁.结果 脑脊液S100B蛋白含量如下:乙脑轻型组522.76(393.35,620.37) pg/ml[极期组609.77(549.27,779.71)pg/ml,恢复期组420.48(344.36,453.19) pg/ml],中型组792.09(705.47,1 108.96) pg/ml[极期组770.19(646.31,1 069.54) pg/ml,恢复期组803.45(602.90,1 396.84) pg/ml],重型组1 021.94(680.84,1 302.15) pg/ml[极期组981.82(680.84,1 826.28) pg/ml,恢复期组989.00(553.62,1 207.67) pg/ml];总极期组762.22 (594.95,1 044.97) pg/ml,总恢复期组581.76 (442.51,1 069.10) pg/ml;MRI未显示病灶组561.52 (454.36,814.56) pg/ml,MRI显示病灶组803.45(602.90,1 104.01) pg/ml;对照组266.71(205.72,390.05) pg/ml.经比较S100B水平在乙脑中型组、重型组、总极期组、总恢复期组、MRI未显示病灶组及MRI显示病灶组都高于对照组(H =4.864、5.497、5.075、3.918、2.971、4.981,P=0.000、0.000、0.000、0.000、0.009、0.000).轻型组S100B浓度低于中、重型组(H=-2.786、-3.514,P=0.032、0.003).结论 S100B蛋白含量与乙脑患者病情轻重、病程及影像学表现有一定关系,动态观察其含量变化,对乙脑患者的病情监测及疗效判断均具有重要意义.
目的 測定流行性乙型腦炎(簡稱乙腦)患者腦脊液中S100B蛋白的含量,探究S100B蛋白在乙腦髮病過程中的意義.方法 選取2013年8-10月在河北醫科大學第二醫院神經內科住院、經河北省疾病控製中心檢測乙腦病毒IgM抗體暘性、確診為乙腦的45例患者,應用ELISA法測量其腦脊液中S100B蛋白濃度.其中男23例,女22例,年齡14 ～72歲,平均(38.8±14.9)歲.按照髮病時間分為初期、極期、恢複期,按臨床癥狀的輕重分為輕型、中型、重型,按影像學資料分為MRI未顯示病竈組、MRI顯示病竈組;選取同期結閤臨床錶現及相關檢查除外中樞神經繫統感染性疾病的患者12例作為對照組,其中男4例,女8例,年齡18～67歲,平均(37.1±14.5)歲.結果 腦脊液S100B蛋白含量如下:乙腦輕型組522.76(393.35,620.37) pg/ml[極期組609.77(549.27,779.71)pg/ml,恢複期組420.48(344.36,453.19) pg/ml],中型組792.09(705.47,1 108.96) pg/ml[極期組770.19(646.31,1 069.54) pg/ml,恢複期組803.45(602.90,1 396.84) pg/ml],重型組1 021.94(680.84,1 302.15) pg/ml[極期組981.82(680.84,1 826.28) pg/ml,恢複期組989.00(553.62,1 207.67) pg/ml];總極期組762.22 (594.95,1 044.97) pg/ml,總恢複期組581.76 (442.51,1 069.10) pg/ml;MRI未顯示病竈組561.52 (454.36,814.56) pg/ml,MRI顯示病竈組803.45(602.90,1 104.01) pg/ml;對照組266.71(205.72,390.05) pg/ml.經比較S100B水平在乙腦中型組、重型組、總極期組、總恢複期組、MRI未顯示病竈組及MRI顯示病竈組都高于對照組(H =4.864、5.497、5.075、3.918、2.971、4.981,P=0.000、0.000、0.000、0.000、0.009、0.000).輕型組S100B濃度低于中、重型組(H=-2.786、-3.514,P=0.032、0.003).結論 S100B蛋白含量與乙腦患者病情輕重、病程及影像學錶現有一定關繫,動態觀察其含量變化,對乙腦患者的病情鑑測及療效判斷均具有重要意義.
목적 측정류행성을형뇌염(간칭을뇌)환자뇌척액중S100B단백적함량,탐구S100B단백재을뇌발병과정중적의의.방법 선취2013년8-10월재하북의과대학제이의원신경내과주원、경하북성질병공제중심검측을뇌병독IgM항체양성、학진위을뇌적45례환자,응용ELISA법측량기뇌척액중S100B단백농도.기중남23례,녀22례,년령14 ～72세,평균(38.8±14.9)세.안조발병시간분위초기、겁기、회복기,안림상증상적경중분위경형、중형、중형,안영상학자료분위MRI미현시병조조、MRI현시병조조;선취동기결합림상표현급상관검사제외중추신경계통감염성질병적환자12례작위대조조,기중남4례,녀8례,년령18～67세,평균(37.1±14.5)세.결과 뇌척액S100B단백함량여하:을뇌경형조522.76(393.35,620.37) pg/ml[겁기조609.77(549.27,779.71)pg/ml,회복기조420.48(344.36,453.19) pg/ml],중형조792.09(705.47,1 108.96) pg/ml[겁기조770.19(646.31,1 069.54) pg/ml,회복기조803.45(602.90,1 396.84) pg/ml],중형조1 021.94(680.84,1 302.15) pg/ml[겁기조981.82(680.84,1 826.28) pg/ml,회복기조989.00(553.62,1 207.67) pg/ml];총겁기조762.22 (594.95,1 044.97) pg/ml,총회복기조581.76 (442.51,1 069.10) pg/ml;MRI미현시병조조561.52 (454.36,814.56) pg/ml,MRI현시병조조803.45(602.90,1 104.01) pg/ml;대조조266.71(205.72,390.05) pg/ml.경비교S100B수평재을뇌중형조、중형조、총겁기조、총회복기조、MRI미현시병조조급MRI현시병조조도고우대조조(H =4.864、5.497、5.075、3.918、2.971、4.981,P=0.000、0.000、0.000、0.000、0.009、0.000).경형조S100B농도저우중、중형조(H=-2.786、-3.514,P=0.032、0.003).결론 S100B단백함량여을뇌환자병정경중、병정급영상학표현유일정관계,동태관찰기함량변화,대을뇌환자적병정감측급료효판단균구유중요의의.
Objective To investigate the role of S100B protein in the pathogenesis of patients with Japanese encephalitis (JE).Methods A total of 45 patients were enrolled in the Second Hospital of Hebei Medical University from August 2013 to October 2013,who were diagnosed as JE on the basis of clinical features and positive IgM antibodies against JE virus measured by enzyme-linked immunosorbent assay (ELISA) from the Center of Disease Control of Shijiazhuang.The JE patients were divided into initial phase group,acute phase group and convalescence group based on the course,mild JE group,moderate JE group and severe JE group based on the severity,MRI-no-lesion group and MRI lesions group based on the imaging findings of JE.Twelve cases with no evidence of infection in central nervous system in the meantime were chosen as control.The S100B protein was measured by ELISA.Results The content of S100B protein in cerebrospinal fluid was as follows:522.76 (393.35,620.37) pg/ml in mild JE group (acute phase group:609.77 (549.27,779.71) pg/ml,convalescence group:420.48 (344.36,453.19) pg/ml),792.09 (705.47,1 108.96) pg/ml in moderate JE group (acute phase group:770.19 (646.31,1 069.54) pg/ml,convalescence group:803.45 (602.90,1 396.84) pg/ml),and 1 021.94 (680.84,1 302.15) pg/ml in severe JE group (acute phase group:981.82 (680.84,1 826.28) pg/ml,convalescence group:989.00 (553.62,1 207.67) pg/ml).The S100B protein content was 561.52 (454.36,814.56) pg/ml,803.45 (602.90,1 104.01) pg/ml,762.22 (594.95,1 044.97) pg/ml,581.76 (442.51,1 069.10) pg/ml in MRI-no-lesion group,MRI lesions group,total acute phase group and total convalescence group,respectively.While in control group,the S100B protein content was 266.71 (205.72,390.05) pg/ml.The contents of S100B protein in moderate JE group,severe JE group,total acute phase group,total convalescence group,MRI-no-lesion group,MRI lesions group were higher than that in control group (H =4.864,5.497,5.075,3.918,2.971,4.981,P =0.000,0.000,0.000,0.000,0.009,0.000).The contents of S100B protein in mild JE group was lower than that in moderate JE group and severe JE group (H =-2.786,-3.514,P =0.032,0.003).Conclusions The level of S100B protein in cerebrospinal fluid is related with the severity,duration and imaging presentation of JE patients.The dynamic monitoring of S100B protein levels is of great significance for assessment of the patients' condition and curative effect.
