CAJ | 학술논문

目的探讨白细胞介素(interleukin,IL)-6单核苷酸基因多态性与中国东北地区汉族人群偏头痛及其亚型之间的相关性.方法收集2012年3月至2013年6月辽宁省人民医院确诊的偏头痛发作期的患者252例,均为中国东北地区汉族人群.同时收集同期的健康体检者及辽宁省人民医院任职的健康职工270名,采用病例对照研究的方法,应用聚合酶链式反应技术及基因测序技术检测偏头痛组[252例;包括无先兆偏头痛(M0)组(168例)和有先兆偏头痛组(84例);男性组(109例)和女性组(143例)]与对照组[270名;包括男性组(116名)和女性组(154名)]及二者的亚组中IL-6-572G/C(rs1800796)位点的基因型及等位基因的频率分布.结果 IL-6-572G/C位点C/C基因型和C等位基因频率在偏头痛组与对照组间的分布差异均有统计学意义[32.54％ (82/252)与19.63％ (53/270),x2=12.061,P =0.002;55.56％ (280/504)与45.37％ (245/540),x2=10.818,P=0.001];C/C基因型和C等位基因频率在偏头痛组与对照组的亚组女性组间进行比较,其分布差异仍有统计学意义[21.83％ (55/252)与12.59％(34/270),x2=10.187,P=0.006;33.73％(170/504)与26.48％ (143/540),x2=10.073,P=0.002].MO组与对照组的C/C基因型和C等位基因频率分布差异具有统计学意义[24.60％ (62/252)与19.63％ (53/270),x2=18.172,P=0.000;39.88％ (201/504)与45.37％ (245/540),x2=17.307,P=0.000].对该位点的C/C基因型频率分布差异进行条件Logistic回归校正后,在偏头痛组与对照组中差异仍有统计学意义(OR=22.861,95％ CI5.218 ～27.098,P=0.023).结论 IL-6-572G/C位点基因多态性与中国东北地区汉族人群偏头痛发作相关,C/C基因型提高了中国东北地区汉族人群偏头痛的易患性.
목적 탐토백세포개소(interleukin,IL)-6단핵감산기인다태성여중국동북지구한족인군편두통급기아형지간적상관성.방법 수집2012년3월지2013년6월요녕성인민의원학진적편두통발작기적환자252례,균위중국동북지구한족인군.동시수집동기적건강체검자급요녕성인민의원임직적건강직공270명,채용병례대조연구적방법,응용취합매련식반응기술급기인측서기술검측편두통조[252례;포괄무선조편두통(M0)조(168례)화유선조편두통조(84례);남성조(109례)화녀성조(143례)]여대조조[270명;포괄남성조(116명)화녀성조(154명)]급이자적아조중IL-6-572G/C(rs1800796)위점적기인형급등위기인적빈솔분포.결과 IL-6-572G/C위점C/C기인형화C등위기인빈솔재편두통조여대조조간적분포차이균유통계학의의[32.54％ (82/252)여19.63％ (53/270),x2=12.061,P =0.002;55.56％ (280/504)여45.37％ (245/540),x2=10.818,P=0.001];C/C기인형화C등위기인빈솔재편두통조여대조조적아조녀성조간진행비교,기분포차이잉유통계학의의[21.83％ (55/252)여12.59％(34/270),x2=10.187,P=0.006;33.73％(170/504)여26.48％ (143/540),x2=10.073,P=0.002].MO조여대조조적C/C기인형화C등위기인빈솔분포차이구유통계학의의[24.60％ (62/252)여19.63％ (53/270),x2=18.172,P=0.000;39.88％ (201/504)여45.37％ (245/540),x2=17.307,P=0.000].대해위점적C/C기인형빈솔분포차이진행조건Logistic회귀교정후,재편두통조여대조조중차이잉유통계학의의(OR=22.861,95％ CI5.218 ～27.098,P=0.023).결론 IL-6-572G/C위점기인다태성여중국동북지구한족인군편두통발작상관,C/C기인형제고료중국동북지구한족인군편두통적역환성.
Objective To investigate the associations of a single-nucleotide polymorphisms (SNPs) in the interleukin (IL)-6 gene with the migraine of a Han Chinese northeastern population.Methods We collected 252 patients of the migraine attack,who were diagnosed at People' s Hospital of Liaoning Province from March 2012 to June 2013,all being Han nationality in the northeast of China.At the same time,we collected 270 healthy people over the same period who had health examination or were healthy workers of People' s Hospital of Liaoning Province.We used polymerase chain reaction and gene sequencing to analyze the genotype and allele frequencies of the-572G/C (rs1800796) IL-6 polymorphisms between migraine cases (n =252;including migraine without aura (MO) group (n =168) and migraine with aura group (n =84);the male group (n =109) and the female group (n =143)) and control group (n =270;the male group (n =116) and the female group (n =152)),as well as analyzed the genotype and allele frequencies distribution between the subgroups.Results We found higher frequencies of the distribution of the C/C genotype and the C allele of the-572G/C polymorphism in migraine cases than in control group (32.54％ (82/252) vs 19.63％ (53/270),x2 =12.061,P =0.002;55.56％ (280/504) vs 45.37％ (245/540),x2 =10.818,P =0.001),higher distribution frequencies of the C/C genotype and the C allele in female group (21.83％ (55/252) vs 12.59％ (34/270),x2 =10.187,P =0.006;33.73％ (170/504) vs 26.48％ (143/540),x2 =10.073,P =0.002).Between MO group and controls,these distribution frequencies had statistically significant difference (24.60％ (62/252) vs 19.63％ (53/270),x2 =18.172,P=0.000;39.88％ (201/504) vs 45.37％ (245/540),x2 =17.307,P=0.000).After correction by conditional Logistic regression,the frequency distribution difference of the C/C genotype between the migraine cases and controls remained statistically significant (OR =22.861,95％ CI 5.218-27.098,P =0.023).Conclusion Our data indicate that the SNPs of the IL-6 are associated with migaine and the C/C genotype increases the migraine susceptibility in a Han Chinese northeastern population.