应用化工
應用化工
응용화공
APPLIED CHEMICAL INDUSTRY
2015年
5期
855-857
,共3页
王小青%李松涛%宫闻婧%尹志峰%王良友
王小青%李鬆濤%宮聞婧%尹誌峰%王良友
왕소청%리송도%궁문청%윤지봉%왕량우
神经降压素%类似物%多肽合成%纯化
神經降壓素%類似物%多肽閤成%純化
신경강압소%유사물%다태합성%순화
neurotensin%analogue%solid phase peptide synthesis%purification
以 Wang Resin 为载体，Fmoc 保护氨基酸为原料，经 DIC/ HOBT 缩合，三氟乙酸（TFA）/间甲酚/乙二硫醇（EDT）裂解体系脱除保护基，经 RP-HPLC 纯化后得到了纯度为98%以上的神经降压素类似物，总收率为39.99%。
以 Wang Resin 為載體，Fmoc 保護氨基痠為原料，經 DIC/ HOBT 縮閤，三氟乙痠（TFA）/間甲酚/乙二硫醇（EDT）裂解體繫脫除保護基，經 RP-HPLC 純化後得到瞭純度為98%以上的神經降壓素類似物，總收率為39.99%。
이 Wang Resin 위재체，Fmoc 보호안기산위원료，경 DIC/ HOBT 축합，삼불을산（TFA）/간갑분/을이류순（EDT）렬해체계탈제보호기，경 RP-HPLC 순화후득도료순도위98%이상적신경강압소유사물，총수솔위39.99%。
The neurotensin analogue was successfully synthesized according to its peptide sequence by using Wang Resin as a solid supporter with Fmoc-amino acids as raw materials and HOBT/ DIC as the coupling reagent. The peptide was cleaved from the resin with trifluoroacetic acid(TFA)in the presence of two additional scavengers m-cresol and EDT. The crude peptide of neurotensin analogue was purified by RP-HPLC. The purity was more than 98% and the total yield was 39. 99% .
