CAJ | 학술논문

研究了无溶剂体系中酶法催化微藻油脂乙酯化制备生物柴油的技术工艺，采用 Box-Behnken 设计及响应面优化了工艺参数。经 SAS 9.2软件分析得到的最优条件为：Novozym 435脂肪酶用量6.0%（w/ w），醇油摩尔比4.0：1，温度44.7℃，反应时间17.6 h。在该条件下，乙酯得率达94.86%。同时，获得的较高乙酯转化率为微藻油脂中多不饱和脂肪酸提取奠定了技术基础。
연구료무용제체계중매법최화미조유지을지화제비생물시유적기술공예，채용 Box-Behnken 설계급향응면우화료공예삼수。경 SAS 9.2연건분석득도적최우조건위：Novozym 435지방매용량6.0%（w/ w），순유마이비4.0：1，온도44.7℃，반응시간17.6 h。재해조건하，을지득솔체94.86%。동시，획득적교고을지전화솔위미조유지중다불포화지방산제취전정료기술기출。
The process of ethyl esterification of microalgae oil in solvent-free system was optimized by Box-Behnken design and response surface method. Analyzed via SAS 9. 2,the optimized conditions were No-vozym 435 dosage 6. 0%(w / w),molar ratio of ethanol to oil 4. 0 : 1,temperature 44. 7 ℃ and reaction duration 17. 6 h,with fatty acid ethyl ester yield of 94. 86% . Meanwhile,a rather high conversion ratio of ethyl esters may lay good foundation for extraction of polyunsaturated fatty acids from microalgae oils.