中华小儿外科杂志
中華小兒外科雜誌
중화소인외과잡지
CHINESE JOURNAL OF PEDIATRIC SURGERY
2015年
5期
357-362
,共6页
髋脱位,先天性%髋臼%软骨%骨形态发生蛋白质类%生物学标记
髖脫位,先天性%髖臼%軟骨%骨形態髮生蛋白質類%生物學標記
관탈위,선천성%관구%연골%골형태발생단백질류%생물학표기
Hip dislocation,congenital%Acetabulum%Cartilage%Bone morphogenetic proteins%Biological markers
目的 研究BMP-2在髋臼软骨发育早期及发育不良髋臼软骨可逆性恢复过程中的作用.方法 通过伸髋内收、模拟襁褓体位固定新生大鼠双后肢,建立发育不良髋臼软骨模型.将髋臼标本经HE染色后观察比较正常及发育不良髋臼软骨组织形态学变化特点,同时用ELISA方法和PCR方法分别检测BMP-2、BMP-4、BMP-6、BMP-7的分泌及基因表达情况.将捆绑不同时间后的大鼠松绑,其中部分当场处死,其余大鼠继续喂养,最终至30日龄,建立发育不良髋臼软骨可逆性恢复模型.研究其髋臼软骨组织形态学恢复及BMP-2分泌变化情况.结果 正常大鼠髋臼软骨呈半圆形、容积大、表面光滑.发育不良髋臼软骨髋臼上缘肥厚,软骨发生变性,与周围组织分界不清.髋臼软骨BMP-2的分泌在正常大鼠7日龄和9日龄时出现高峰,分别为(13.7±0.29) ng/ml和(13.9±0.38) ng/ml.而在发育不良髋臼软骨中这一分泌高峰消失.在发育不良髋臼软骨可逆性恢复组,捆绑4d和6d的大鼠,BMP-2的分泌高峰出现延迟,都在15日龄时出现;而在捆绑8d及以上的大鼠,在松绑后继续喂养至30日龄,髋臼软骨组织形态无法恢复正常,并且BMP-2的分泌高峰未出现.结论 BMP-2的分泌可能是髋臼软骨早期发育情况的生物学标记之一.
目的 研究BMP-2在髖臼軟骨髮育早期及髮育不良髖臼軟骨可逆性恢複過程中的作用.方法 通過伸髖內收、模擬繈褓體位固定新生大鼠雙後肢,建立髮育不良髖臼軟骨模型.將髖臼標本經HE染色後觀察比較正常及髮育不良髖臼軟骨組織形態學變化特點,同時用ELISA方法和PCR方法分彆檢測BMP-2、BMP-4、BMP-6、BMP-7的分泌及基因錶達情況.將捆綁不同時間後的大鼠鬆綁,其中部分噹場處死,其餘大鼠繼續餵養,最終至30日齡,建立髮育不良髖臼軟骨可逆性恢複模型.研究其髖臼軟骨組織形態學恢複及BMP-2分泌變化情況.結果 正常大鼠髖臼軟骨呈半圓形、容積大、錶麵光滑.髮育不良髖臼軟骨髖臼上緣肥厚,軟骨髮生變性,與週圍組織分界不清.髖臼軟骨BMP-2的分泌在正常大鼠7日齡和9日齡時齣現高峰,分彆為(13.7±0.29) ng/ml和(13.9±0.38) ng/ml.而在髮育不良髖臼軟骨中這一分泌高峰消失.在髮育不良髖臼軟骨可逆性恢複組,捆綁4d和6d的大鼠,BMP-2的分泌高峰齣現延遲,都在15日齡時齣現;而在捆綁8d及以上的大鼠,在鬆綁後繼續餵養至30日齡,髖臼軟骨組織形態無法恢複正常,併且BMP-2的分泌高峰未齣現.結論 BMP-2的分泌可能是髖臼軟骨早期髮育情況的生物學標記之一.
목적 연구BMP-2재관구연골발육조기급발육불량관구연골가역성회복과정중적작용.방법 통과신관내수、모의강보체위고정신생대서쌍후지,건립발육불량관구연골모형.장관구표본경HE염색후관찰비교정상급발육불량관구연골조직형태학변화특점,동시용ELISA방법화PCR방법분별검측BMP-2、BMP-4、BMP-6、BMP-7적분비급기인표체정황.장곤방불동시간후적대서송방,기중부분당장처사,기여대서계속위양,최종지30일령,건립발육불량관구연골가역성회복모형.연구기관구연골조직형태학회복급BMP-2분비변화정황.결과 정상대서관구연골정반원형、용적대、표면광활.발육불량관구연골관구상연비후,연골발생변성,여주위조직분계불청.관구연골BMP-2적분비재정상대서7일령화9일령시출현고봉,분별위(13.7±0.29) ng/ml화(13.9±0.38) ng/ml.이재발육불량관구연골중저일분비고봉소실.재발육불량관구연골가역성회복조,곤방4d화6d적대서,BMP-2적분비고봉출현연지,도재15일령시출현;이재곤방8d급이상적대서,재송방후계속위양지30일령,관구연골조직형태무법회복정상,병차BMP-2적분비고봉미출현.결론 BMP-2적분비가능시관구연골조기발육정황적생물학표기지일.
Objective To explore the early-stage acetabulum development in normal and dysplastic acetabula and elucidate the function of bone morphogenetic protein 2 (BMP-2) in early acetabulum development and dysplastic acetabulum remodeling.Methods The rat model of dysplastic acetabulum was established by maintaining hips in a swaddling position.By analyzing the cartilage histologic characteristics,early-stage acetabulum developments were examined in normal and dysplastic acetabulum animals.Meantime,the mRNA expression and chondrocyte secretion of functional BMP-2,bone morphogenetic protein 4 (BMP-4),bone morphogenetic protein 6 (BMP-6) and bone morphogenetic protein 7 (BMP-7) were detected at different postnatal timepoints using realtime fluorescence quantitative polymerase chain reaction and enzyme-linked immunosorbent assay respectively.Then the cartilage morphologic remodeling was observed in banding removal group.The mRNA expression and chondrocytic secretion of functional BMP-2 were detected in chondrocytes of each acetabulum.Results Normal acetabulum was semispheric with a large volume and a smooth surface while dysplastic acetabulum had a thickened superior border with degenerated cartilage.In normal rats,two secretion peaks of (13.7 ± 0.29) and (13.9 ± 0.38) ng/ml occurred at Days 7 and 9.And the difference was statistically significant.In dysplastic acetabulum group,this peak disappeared.As for banding removal group,acetabulum developed almost normally at Day 30 after released banding at Days 2,4 and 6.But for acetabula of rats released at Days 8 and 10,histomorphology indicated dysplastic acetabula.For acetabula in banding release group,the secretion peaks of BMP-2 in acetabula released at Days 2,4 and 6 appeared later than normal.For acetabula released at Day 8 and later,the secretion peak of BMP-2 disappeared.Conclusions The secretion peak of BMP-2 may accelerate the early development of acetabula so that it is possibly a biomarker of acetabulum development.
