中国现代医生
中國現代醫生
중국현대의생
CHINA MODERN DOCTOR
2015年
16期
18-20
,共3页
胚胎培养技术%胚胎发育%mWOW法
胚胎培養技術%胚胎髮育%mWOW法
배태배양기술%배태발육%mWOW법
Embryo culture techniques%Embryonic development%mWOW method
目的:探讨不同体外培养方法对小鼠去透明带胚胎发育的影响。方法以胚胎各阶段发育率、囊胚率和囊胚细胞数作为衡量指标,对比微滴单卵法、微滴群卵法、微滴单卵+群卵法、mWOW培养法(the modified “well of well”system)四种体外培养方法,探讨不同体外培养方法对去透明带胚胎发育的影响。结果 mWOW培养法、微滴单卵+群卵法、微滴单卵法这三种方法之间的发育率、囊胚率以及囊胚数均有显著差异(P<0.05)。结论mWOW法与其他三种方法相比,更适合作为体外培养去透明带小鼠8-细胞以后阶段的方法,以及用于体内移植小鼠胚胎的体外培养方法。
目的:探討不同體外培養方法對小鼠去透明帶胚胎髮育的影響。方法以胚胎各階段髮育率、囊胚率和囊胚細胞數作為衡量指標,對比微滴單卵法、微滴群卵法、微滴單卵+群卵法、mWOW培養法(the modified “well of well”system)四種體外培養方法,探討不同體外培養方法對去透明帶胚胎髮育的影響。結果 mWOW培養法、微滴單卵+群卵法、微滴單卵法這三種方法之間的髮育率、囊胚率以及囊胚數均有顯著差異(P<0.05)。結論mWOW法與其他三種方法相比,更適閤作為體外培養去透明帶小鼠8-細胞以後階段的方法,以及用于體內移植小鼠胚胎的體外培養方法。
목적:탐토불동체외배양방법대소서거투명대배태발육적영향。방법이배태각계단발육솔、낭배솔화낭배세포수작위형량지표,대비미적단란법、미적군란법、미적단란+군란법、mWOW배양법(the modified “well of well”system)사충체외배양방법,탐토불동체외배양방법대거투명대배태발육적영향。결과 mWOW배양법、미적단란+군란법、미적단란법저삼충방법지간적발육솔、낭배솔이급낭배수균유현저차이(P<0.05)。결론mWOW법여기타삼충방법상비,경괄합작위체외배양거투명대소서8-세포이후계단적방법,이급용우체내이식소서배태적체외배양방법。
Objective To investigate the effects of different culture methods in vitro mouse zona-free embryonic devel-opment. Methods In various stages of embryonic development rate,blastocyst rate and cell numbers of blastocysts de-veloped were measured as mWOW system (the modified “well of well” system), micro-drops single + group, micro-drops single and micro-drops group culture in vitro. The effects of different methods of were compared in vitro embryo development zona-free. Results Embryonic development rate,blastocyst rate and cell numbers of blastocysts developed were significantly higher in mWOW system culture than micro-drops single + group, micro-drops single and micro-drops group culture. Conclusion Compared with micro-drops single+group,micro-drops single and micro-drops group culture,mWOW system method is more suitable for zona-free cultured in vitro mouse embryos.