CAJ | 학술논문

目的：探讨莪术醇β-环糊精包合物作用对肝癌细胞的增殖与凋亡及细胞周期的影响及其分子机制。方法采用不同浓度莪术醇β-环糊精包合物处理肝癌Bel-7404细胞后,噻唑蓝[3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide, MTT]比色法测定细胞增殖,流式细胞仪检测细胞周期与凋亡情况。Western blot检测细胞周期调控相关基因的表达水平。结果 MTT法检测结果显示,与对照组比较,莪术醇β-环糊精包合物各剂量组生长抑制率均明显升高,呈剂量与时间依赖性,差异有统计学意义(P<0.05)。流式细胞术检测结果显示,莪术醇β-环糊精包合物处理促进细胞凋亡(P<0.05),并使细胞阻滞在G0/G1期(P<0.05),伴随p21WAF1及p27KIP1的表达水平升高。结论莪术醇β-环糊精包合物能够抑制肝癌细胞Bel-7404的增殖,促进凋亡,并通过上调p21WAF1及p27KIP1基因的表达而诱导G1期阻滞。
목적：탐토아술순β-배호정포합물작용대간암세포적증식여조망급세포주기적영향급기분자궤제。방법채용불동농도아술순β-배호정포합물처리간암Bel-7404세포후,새서람[3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide, MTT]비색법측정세포증식,류식세포의검측세포주기여조망정황。Western blot검측세포주기조공상관기인적표체수평。결과 MTT법검측결과현시,여대조조비교,아술순β-배호정포합물각제량조생장억제솔균명현승고,정제량여시간의뢰성,차이유통계학의의(P<0.05)。류식세포술검측결과현시,아술순β-배호정포합물처리촉진세포조망(P<0.05),병사세포조체재G0/G1기(P<0.05),반수p21WAF1급p27KIP1적표체수평승고。결론아술순β-배호정포합물능구억제간암세포Bel-7404적증식,촉진조망,병통과상조p21WAF1급p27KIP1기인적표체이유도G1기조체。
Objective To investigate the effects and mechanisms of curcumol-β-cyclodextrin inclusion compound on the proliferation, apoptosis, and cell cycle of human hepatocarcinoma cell line Bel-7404. Methods The effects of dif-ferent dose of curcumol-β-cyclodextrin inclusion compound on proliferation of human hepatocarcinoma cell line Bel-7404 in vitro was analyzed by MTT assay(P<0.05). The cell cycles and apoptosis were detected by flow cytometer(P<0.05). Expression of cycle regulation-related genes were assessed by Western blot. Results Compared with control groups, MTT results showed that curcumol-β-cyclodextrin inclusion compound significantly inhibited the proliferation of Bel-7404 cells in a dose- and time-dependent manner. The results of flow cytometry showed that curcumol-β-cy-clodextrin inclusion compound resulted in the cell cycle arrest at G0/G1 phase and increased the apoptotic cell fraction(P<0.05). Western blotting results showed that curcumol-β-cyclodextrin inclusion compound increased the expression of p21WAF1 and p27KIP1. Conclusion Curcumol-β-cyclodextrin inclusion compound inhibits the growth and induces apoptosis and G0/G1 phase arrest of Bel-7404 in vitro. The mechanisms of G1 arrest may be related to the regulation of the p21WAF1 and p27KIP1.