中国现代医生
中國現代醫生
중국현대의생
CHINA MODERN DOCTOR
2015年
16期
4-7
,共4页
急性肾损伤%脓毒症%生大黄%Netrin-1
急性腎損傷%膿毒癥%生大黃%Netrin-1
급성신손상%농독증%생대황%Netrin-1
Acute kidney injury%Sepsis%Rhubarb%Netrin-1
目的：探讨脓毒症急性肾损伤的早期诊断指标及生大黄对其的保护作用。方法将60只大鼠按随机数字表法分为模型组(A)、治疗组(B)和对照组(C)三组,每组20只。模型组腹膜内注射脂多糖5 mg/kg诱导建立AKI模型,治疗组在腹膜内注射脂多糖即刻胃管内注入生大黄粉溶液,对照组腹膜内注射等量生理盐水。三组大鼠分别在处理前即刻(基础值,0 h),处理后1、6、24 h测定尿Netrin-1及尿KIM-1含量,同时采集血样测定肌酐含量。结果大鼠注射脂多糖6 h后,血清肌酐及尿KIM-1水平开始明显上升,注射后1 h尿Netrin-1即有明显上升,6 h达到峰值,24 h左右逐渐下降；生大黄治疗组大鼠各时间点血清肌酐、尿Netrin-1及尿KIM-1上升程度均较模型组明显降低(P<0.05)。结论尿Netrin-1对脓毒症急性肾损伤有早期诊断作用,生大黄可降低脓毒症急性肾损伤大鼠Scr、尿Netrin-1及尿KIM-1水平,对肾脏有保护作用。
目的：探討膿毒癥急性腎損傷的早期診斷指標及生大黃對其的保護作用。方法將60隻大鼠按隨機數字錶法分為模型組(A)、治療組(B)和對照組(C)三組,每組20隻。模型組腹膜內註射脂多糖5 mg/kg誘導建立AKI模型,治療組在腹膜內註射脂多糖即刻胃管內註入生大黃粉溶液,對照組腹膜內註射等量生理鹽水。三組大鼠分彆在處理前即刻(基礎值,0 h),處理後1、6、24 h測定尿Netrin-1及尿KIM-1含量,同時採集血樣測定肌酐含量。結果大鼠註射脂多糖6 h後,血清肌酐及尿KIM-1水平開始明顯上升,註射後1 h尿Netrin-1即有明顯上升,6 h達到峰值,24 h左右逐漸下降；生大黃治療組大鼠各時間點血清肌酐、尿Netrin-1及尿KIM-1上升程度均較模型組明顯降低(P<0.05)。結論尿Netrin-1對膿毒癥急性腎損傷有早期診斷作用,生大黃可降低膿毒癥急性腎損傷大鼠Scr、尿Netrin-1及尿KIM-1水平,對腎髒有保護作用。
목적：탐토농독증급성신손상적조기진단지표급생대황대기적보호작용。방법장60지대서안수궤수자표법분위모형조(A)、치료조(B)화대조조(C)삼조,매조20지。모형조복막내주사지다당5 mg/kg유도건립AKI모형,치료조재복막내주사지다당즉각위관내주입생대황분용액,대조조복막내주사등량생리염수。삼조대서분별재처리전즉각(기출치,0 h),처리후1、6、24 h측정뇨Netrin-1급뇨KIM-1함량,동시채집혈양측정기항함량。결과대서주사지다당6 h후,혈청기항급뇨KIM-1수평개시명현상승,주사후1 h뇨Netrin-1즉유명현상승,6 h체도봉치,24 h좌우축점하강；생대황치료조대서각시간점혈청기항、뇨Netrin-1급뇨KIM-1상승정도균교모형조명현강저(P<0.05)。결론뇨Netrin-1대농독증급성신손상유조기진단작용,생대황가강저농독증급성신손상대서Scr、뇨Netrin-1급뇨KIM-1수평,대신장유보호작용。
Objective To study early diagnostic index of septic acute kidney injury(AKI) and protective effect of rhubarb on septic AKI. Methods Sixty rats were randomly divided into the following 3 groups:model group (A), treatment group (B) and control group (C). Each group contains 20 rats. Rats from group A were received a intraperitoneal injection of lipopolysaccharide (LPS) at a dose of 5 mg/kg to induce AKI. Rats from group B were given rhubarb powder solution by oral administration immediately after LPS injection. Rats from group C were treated with the same volume of normal saline (NS) by intraperitoneal injection. Both serum level of creatinine (Scr) and urinary levels of Netrin-1 and KIM-1 of rats in 3 groups were detected in the different time points of treatment (0 h, 1 h, 6 h and 24 h). Results The levels of KIM-1 and CR in rats treated with LPS were obviously increased at the time point of 6 h (P<0.05). The urinary lev-el of Netrin-1 began to increase at the time point of 1 h and reached the highest level at the time point of 6 h, then gradually decreased at the time point of 24 h after the injection of LPS. In group B, the levels of Scr, Netrin-1 and KIM-1 were significantly lower as compared with group A (P<0.05). Conclusion The urinary level of Netrin-1 might has early diagnostic value in septic acute kidney injury. Rhubarb could protect the kidney of AKI model rats by de-creasing the serum level of Scr, urinary levels of Netrin-1 and KIM-1.
