CAJ | 학술논문

目的：：采用HPLC法测定高良姜素的含量，从而评价高良姜素分子印迹萃取柱的分离效率。方法：以高良姜素为模板分子，丙烯酰胺为功能单体，丙酮为致孔剂，乙二醇二甲基丙烯酸酯为交联剂，制备高良姜素分子印迹聚合物，采用HPLC法测定分离出的高良姜素的含量，以评价分子印迹萃取柱的分离效率。结果：高良姜素在1.0-5.0μg/ml范围内呈良好的线性关系，线性方程为y=17014x+457.2，r=0.9989(n=5)。高良姜素的平均含量为0.91%，RSD为2.4%(n=6)。结论：高良姜素分子印迹萃取柱的分离效率较高，可以很好的分离高良姜饮片提取液中的高良姜素。
목적：：채용HPLC법측정고량강소적함량，종이평개고량강소분자인적췌취주적분리효솔。방법：이고량강소위모판분자，병희선알위공능단체，병동위치공제，을이순이갑기병희산지위교련제，제비고량강소분자인적취합물，채용HPLC법측정분리출적고량강소적함량，이평개분자인적췌취주적분리효솔。결과：고량강소재1.0-5.0μg/ml범위내정량호적선성관계，선성방정위y=17014x+457.2，r=0.9989(n=5)。고량강소적평균함량위0.91%，RSD위2.4%(n=6)。결론：고량강소분자인적췌취주적분리효솔교고，가이흔호적분리고량강음편제취액중적고량강소。
Objective:To evaluate the separation efficiency of Galangin molecular imprinted polymers by detecting the Galangin contents with HPLC.Methods:The Galangin molecular imprinted polymers were prepared by using Galangin as template molecule, acrylamide as functional monomers, acetone as pore-foaming agent, ethylene glycol dimethyl acrylic ester as crosslinking agent. The separation efficiency of Galangin molecular imprinted polymers was evaluated by detecting the Galangin content by HPLC.Results:The linear range of Galangin was 1.0-5.0 μg/ml, the linear equation was y=17014x+457.2 (r=0.9989, n=5).The average content of Galangin was 0.91% (RSD=2.4%, n=6).Conclusions: The separation efficiency of Galangin molecular imprinted polymers is higher; and it can separate the Galangin inAlpinia officinarum well.