肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2015年
5期
316-319
,共4页
朱莺娇%赖晓兰%赵珅%郑建伟%王新利%翁小娇%陈强%林小燕
硃鶯嬌%賴曉蘭%趙珅%鄭建偉%王新利%翁小嬌%陳彊%林小燕
주앵교%뢰효란%조신%정건위%왕신리%옹소교%진강%림소연
B16-F10黑色素瘤细胞%CB6F1小鼠%转移%肿瘤模型
B16-F10黑色素瘤細胞%CB6F1小鼠%轉移%腫瘤模型
B16-F10흑색소류세포%CB6F1소서%전이%종류모형
B16-F10 melanoma cells%CB6F1 mice%Metastasis%Tumor model
目的 在CB6F1小鼠上建立黑色素瘤B16-F10细胞皮下移植瘤和肺转移瘤模型,为黑色素瘤的单倍体相合淋巴细胞输注的实验研究提供新的动物模型.方法 将B16-F10细胞株于CB6F1小鼠皮下及尾静脉注射接种,将小鼠按随机数字表法分成3组,即高剂量组(2× 105细胞)、中剂量组(1×105细胞)、低剂量组(5×104细胞),每组8只.筛选出最适接种细胞数后,分别建立皮下移植瘤和肺转移瘤模型,实验重复3次,观察成瘤率和荷瘤鼠的生存时间.结果 在皮下种植瘤模型中,高、中、低剂量组的小鼠成瘤分别为8、8、6只;在肺转移瘤模型中,高、中、低剂量组的小鼠肺转移瘤分别为8、8、5只.以1×105细胞接种建立的皮下移植瘤和肺转移瘤模型重复3次,每次5只小鼠均成瘤.结论 建立了一个成瘤率高、操作简单、稳定的小鼠黑色素瘤模型,为研究单倍体相合淋巴细胞输注提供理想的小鼠实体瘤模型.
目的 在CB6F1小鼠上建立黑色素瘤B16-F10細胞皮下移植瘤和肺轉移瘤模型,為黑色素瘤的單倍體相閤淋巴細胞輸註的實驗研究提供新的動物模型.方法 將B16-F10細胞株于CB6F1小鼠皮下及尾靜脈註射接種,將小鼠按隨機數字錶法分成3組,即高劑量組(2× 105細胞)、中劑量組(1×105細胞)、低劑量組(5×104細胞),每組8隻.篩選齣最適接種細胞數後,分彆建立皮下移植瘤和肺轉移瘤模型,實驗重複3次,觀察成瘤率和荷瘤鼠的生存時間.結果 在皮下種植瘤模型中,高、中、低劑量組的小鼠成瘤分彆為8、8、6隻;在肺轉移瘤模型中,高、中、低劑量組的小鼠肺轉移瘤分彆為8、8、5隻.以1×105細胞接種建立的皮下移植瘤和肺轉移瘤模型重複3次,每次5隻小鼠均成瘤.結論 建立瞭一箇成瘤率高、操作簡單、穩定的小鼠黑色素瘤模型,為研究單倍體相閤淋巴細胞輸註提供理想的小鼠實體瘤模型.
목적 재CB6F1소서상건립흑색소류B16-F10세포피하이식류화폐전이류모형,위흑색소류적단배체상합림파세포수주적실험연구제공신적동물모형.방법 장B16-F10세포주우CB6F1소서피하급미정맥주사접충,장소서안수궤수자표법분성3조,즉고제량조(2× 105세포)、중제량조(1×105세포)、저제량조(5×104세포),매조8지.사선출최괄접충세포수후,분별건립피하이식류화폐전이류모형,실험중복3차,관찰성류솔화하류서적생존시간.결과 재피하충식류모형중,고、중、저제량조적소서성류분별위8、8、6지;재폐전이류모형중,고、중、저제량조적소서폐전이류분별위8、8、5지.이1×105세포접충건립적피하이식류화폐전이류모형중복3차,매차5지소서균성류.결론 건립료일개성류솔고、조작간단、은정적소서흑색소류모형,위연구단배체상합림파세포수주제공이상적소서실체류모형.
Objective To established subcutaneous tumor and pulmonary metastasis models with B16-F10 melanoma in CB6F1 mice,and provide a new melanoma-bearing mice model for haploidentical lymphocytes infusion.Methods CB6F1 mice were inoculated subcutaneously and injected intravenously by tail vein with B16-F10 melanoma cells.According to different number of inoculated B16-F10 cells,CB6F1 mice were randomly divided into three groups (high dose group:2×105 cells; medium dose group:1×105 cells;low dose group:5×104 cells),and chose the best fit number of inoculated cells for establishing subcutaneous transplanted and metastatic melanoma models.Experiments were repeated three times.The incidence of tumorgenesis and survival time of tumor-bearing mice were examined.Results In the subcutaneous tumor models,the incidence of tumorgenesis were 8,8 and 6 in high,medium and low dose group,respectively.In the pulmonary metastasis models,the incidence of tumorgenesis were 8,8 and 5 in high,medium and low dose group,respectively.Then 1×105 cells were injected into mice,and the incidence of tumorgenesis were all 8/8 in both groups.Conclusion The results show that ideal melanoma-bearing mice models for haploidentical lymphocytes infusion are successfully established.These models are convenient,repeatable,and have high rate of bearing tumor.
