CAJ | 학술논문

针对导入基因标记后杂交诱导产生的玉米籽粒，设计了一套基于计算机视觉的玉米单倍体高通量自动分选系统。系统主要由传动部件、斜面翻滚部件、信号采集与处理部件，以及籽粒分选部件4部分组成。斜面翻滚部件表面有凹凸纹理，可使玉米籽粒在下落翻滚过程中产生多种姿态，试验使用这一方法获得含胚面图片的成功率达90％。信号采集与处理部件通过提取玉米籽粒胚面的 SIFT 特征来判断籽粒属性，并将属性结果发送给籽粒分选部件，分选部件根据结果控制直线滑台的运动以实现籽粒筛选。试验结果表明：系统对单倍体的正确识别率为95％，可很好地实现玉米单倍体的自动分选。
침대도입기인표기후잡교유도산생적옥미자립，설계료일투기우계산궤시각적옥미단배체고통량자동분선계통。계통주요유전동부건、사면번곤부건、신호채집여처리부건，이급자립분선부건4부분조성。사면번곤부건표면유요철문리，가사옥미자립재하락번곤과정중산생다충자태，시험사용저일방법획득함배면도편적성공솔체90％。신호채집여처리부건통과제취옥미자립배면적 SIFT 특정래판단자립속성，병장속성결과발송급자립분선부건，분선부건근거결과공제직선활태적운동이실현자립사선。시험결과표명：계통대단배체적정학식별솔위95％，가흔호지실현옥미단배체적자동분선。
A high-throughput maize haploid screening system was designed based on computer vision, which automatical-ly separated the maize haploid kernels after hybrid induction with genetic marks.The system was composed of four parts:the transmission module, the rugged slope module, the signal acquisition &processing module and the kernel separating module.The uneven surface texture was made on the rugged slope module, in order to get various gestures of maize ker-nel during their falling process.Now, success ratio for getting pictures with embryo was 90%.The signal acquisition &processing module identified maize kernel by SIFT extracted from maize embryo, then transmitted the classification infor-mation to the kernel separating module.Maize kernels were separated by the movement of straight slipway according to the classification result.Test results showed that the correct recognition rate of maize haploid was 95%, and the automat-ic separation could be successfully realized by this system.