CAJ | 학술논문

以垦芸2号芸豆品种为原料，以自来水和纯净水作对比试验，研究小分子团磁化水促进芸豆清蛋白的浸出作用，并优化了提取技术参数。通过对提取温度、提取时间、液料比和粉碎粒度四个因素的单因素试验和响应面分析，结果表明：提取温度31℃，提取时间2 h，液料比22∶1，粉碎粒度60目时芸豆清蛋白的提取率均最高，其中小分子团磁化水组提取率为48.96%，高于自来水组（42.79%）和纯净水组（40.87%）；并对小分子团磁化水提取的芸豆清蛋白进行SDS-PAGE电泳测试，得出分子量排布主要集中在42.01 kDa左右，说明所得蛋白为清蛋白。
이은예2호예두품충위원료，이자래수화순정수작대비시험，연구소분자단자화수촉진예두청단백적침출작용，병우화료제취기술삼수。통과대제취온도、제취시간、액료비화분쇄립도사개인소적단인소시험화향응면분석，결과표명：제취온도31℃，제취시간2 h，액료비22∶1，분쇄립도60목시예두청단백적제취솔균최고，기중소분자단자화수조제취솔위48.96%，고우자래수조（42.79%）화순정수조（40.87%）；병대소분자단자화수제취적예두청단백진행SDS-PAGE전영측시，득출분자량배포주요집중재42.01 kDa좌우，설명소득단백위청단백。
Using Kenyun 2 kidney beans as raw material and tap water and pure water as comparison tests,the infusion effects of magnetized water with small molecules mass to kidney bean were studied,and the extraction parameters of albumin from kidney bean were optimized. Through single factor and response surface analysis of four factors such as extraction temperature,extraction time, ratio of liquid to material and particle size,the results showed that the optimum extraction conditions was as follows:extraction temperature 31 ℃,extraction time 2 h,ratio of liquid to material 22∶1,particle size 60 mesh. Under such conditions,the extraction yield of magnetized water with small molecules group was 48.96% and higher than that of tap water group (42.79%)and purified water group (40.87%).SDS-PAGE result showed that the molecular weight of the extracted protein was about 42.01 kDa,and the protein was albumin.