CAJ | 학술논문

通过测定SD大鼠血浆及肝组织中六种酶的活性或含量，探索对乙酰氨基酚短期内对大鼠肝脏的损伤作用。SD大鼠实验组分别皮下注射75、150、250 mg·kg-1剂量的对乙酰氨基酚，对照组注射生理盐水且于给药后1、6、24 h分别采取血浆和肝组织，采用比色法检测各项指标。结果显示：高剂量组大鼠血浆中AST（356.72±43.87 U·L-1）和ALT（325±40.56 U·L-1）以及肝匀浆中GSH（4.55±0.53 mmol·prot-1）、SOD（90.1±12.23 U·g-1）和MDA（230.24±40.43 nmol·g-1）与对照组相比，差异极显著（P＜0.01），说明250 mg·kg-1剂量的对乙酰氨基酚可对大鼠肝脏产生急性损伤作用。
통과측정SD대서혈장급간조직중륙충매적활성혹함량，탐색대을선안기분단기내대대서간장적손상작용。SD대서실험조분별피하주사75、150、250 mg·kg-1제량적대을선안기분，대조조주사생리염수차우급약후1、6、24 h분별채취혈장화간조직，채용비색법검측각항지표。결과현시：고제량조대서혈장중AST（356.72±43.87 U·L-1）화ALT（325±40.56 U·L-1）이급간균장중GSH（4.55±0.53 mmol·prot-1）、SOD（90.1±12.23 U·g-1）화MDA（230.24±40.43 nmol·g-1）여대조조상비，차이겁현저（P＜0.01），설명250 mg·kg-1제량적대을선안기분가대대서간장산생급성손상작용。
In order to illustrate the acetaminophen role on rat liver damage,the activity or levels of 6 kinds of enzyme were detected in rat plasma and liver tissue. SD rats were injected subcutaneously 75,150,250 mg·kg-1 dose of acetaminophen in experimental group,and injected with administered saline after 1,6 and 24 h in control group,and plasma and liver tissue were used colorimetric detection indicators. The high group of rat plasma AST(356.72±43.87 U·L-1)and ALT(325±40.56 U·L-1)and in liver homogenates of GSH(4.55±0.53 mmol·prot-1),SOD(90.1±12.23 U·g-1)and MDA(230.24±40.43 nmol·g-1)compared with the control group,and the difference was significant(P<0.01).In the experiment,250 mg·kg-1 dose of acetaminophen could damage the liver in rats.