黑龙江八一农垦大学学报
黑龍江八一農墾大學學報
흑룡강팔일농은대학학보
JOURNAL OF HEILONGJIANG AUGUST FIRST LAND RECLAMATION UNIVERSITY
2015年
3期
36-39
,共4页
徐昊%孙海英%翟军军%薛洋洋%徐剑华%任超%穆昱%杨玉英
徐昊%孫海英%翟軍軍%薛洋洋%徐劍華%任超%穆昱%楊玉英
서호%손해영%적군군%설양양%서검화%임초%목욱%양옥영
牛肉%沙门氏菌%分离鉴定%药敏试验
牛肉%沙門氏菌%分離鑒定%藥敏試驗
우육%사문씨균%분리감정%약민시험
beef%Salmonella%separating identification%drug resistance
为了解大庆市地区屠宰牛肉沙门氏菌的污染情况,采用流行病学的调查方法对大庆市农贸市场的牛肉进行沙门氏菌鉴定。在大庆市5个销售市场即新村区、龙凤区、大同区、萨尔图区、红岗区分别采样,对牛肉组织进行表面和深部涂板培养。采用选择培养基,染色镜检,生化鉴定,PCR的方法对样品中的沙门氏菌进行分离鉴定,对分离菌株进行药敏实验。在五个地区31份样品中,只有龙凤区的1份样品表面有沙门氏菌污染。检出率为3.2%。
為瞭解大慶市地區屠宰牛肉沙門氏菌的汙染情況,採用流行病學的調查方法對大慶市農貿市場的牛肉進行沙門氏菌鑒定。在大慶市5箇銷售市場即新村區、龍鳳區、大同區、薩爾圖區、紅崗區分彆採樣,對牛肉組織進行錶麵和深部塗闆培養。採用選擇培養基,染色鏡檢,生化鑒定,PCR的方法對樣品中的沙門氏菌進行分離鑒定,對分離菌株進行藥敏實驗。在五箇地區31份樣品中,隻有龍鳳區的1份樣品錶麵有沙門氏菌汙染。檢齣率為3.2%。
위료해대경시지구도재우육사문씨균적오염정황,채용류행병학적조사방법대대경시농무시장적우육진행사문씨균감정。재대경시5개소수시장즉신촌구、룡봉구、대동구、살이도구、홍강구분별채양,대우육조직진행표면화심부도판배양。채용선택배양기,염색경검,생화감정,PCR적방법대양품중적사문씨균진행분리감정,대분리균주진행약민실험。재오개지구31빈양품중,지유룡봉구적1빈양품표면유사문씨균오염。검출솔위3.2%。
In order to understand the pollution of salmonella of slaughter beef in Daqing city,Salmonella of farming market beef was identified by the epidemiological methods. Beef samples were collected from 5 areas of Daqing city,including Xincun district, Longfeng district,Datong district,Saertu district,Honggang district. The bacteria in the surface and deep samples were obtained by plate culturing method,and salmonella were isolated and identified by differential medium,gram staining microscopic examination, biochemical characterization and PCR,and drug resistance of Salmonella in samples were analyzed by drug sensitivity test. In 31 samples from 5 regions,only one sample from Longfeng district was contaminated by Salmonella and the detection rate was 3.2%.