CAJ | 학술논문

目的观察高脂血症叠加抑郁症大鼠open-field行为学特征.方法 40只SD大鼠按随机数字表法分为:正常饮食组(CON)、正常饮食+慢性应激组(CON+ CUMS)、高脂饮食组(HFD)、高脂饮食+慢性应激组(HFD+ CUMS),每组各10只.CON+ CUMS组正常饮食9周时,采用慢性不可预见性轻度应激(CUMS)4周建立抑郁症模型;HFD+ CUMS组高脂饮食9周时,在此基础上叠加CUMS4周,建立高脂血症与抑郁症叠加模型.基线、9周、13周不同时点予血脂、open-field行为学测定.结果 (1)血脂:9周时HFD、HFD+ CUMS两组大鼠血脂异常,如血清总胆固醇[(2.67±0.04)mmoL/L,(2.68 ±0.02) mmol/L]、低密度脂蛋白[(1.08±0.03) mmol/L,(1.06±0.01) mmol/L]明显高于CON、CON+ CUMS组[(1.78±0.12) mmol/L,(0.79±0.04) mmol/L;(1.76±0.09) mmol/L,(0.76±0.06) mmol/L,均P＜0.01],形成典型高脂血症模型.(2)open-field实验:13周时,CON+CUMS组大鼠与CON组大鼠相比,前者第1分钟内运动格子的总数目增多,5 min内运动的中间格子数目、后肢直立次数均减少,且明显增加修饰次数及大便粒数(均P＜0.05);HFD组大鼠仅在5 min内运动总格子、中间及周边格子数目与CON组大鼠有差异(均P＜0.05).HFD+ CUMS组大鼠13周时该实验所有指标均较基线、9周显著减少(均P ＜0.001).13周时,HFD +CUMS组大鼠5 min内运动总格子、中间格子及周边格子数目比其他对照组大鼠均减少,后肢直立次数减少,第1分钟内运动格子的总数目、修饰次数、大便粒数明显增加(均P ＜0.001).结论高脂血症未加控制基础之上,一旦接触慢性应激因子会出现严重抑郁症状,并伴明显自主神经功能紊乱症状.
목적 관찰고지혈증첩가억욱증대서open-field행위학특정.방법 40지SD대서안수궤수자표법분위:정상음식조(CON)、정상음식+만성응격조(CON+ CUMS)、고지음식조(HFD)、고지음식+만성응격조(HFD+ CUMS),매조각10지.CON+ CUMS조정상음식9주시,채용만성불가예견성경도응격(CUMS)4주건립억욱증모형;HFD+ CUMS조고지음식9주시,재차기출상첩가CUMS4주,건립고지혈증여억욱증첩가모형.기선、9주、13주불동시점여혈지、open-field행위학측정.결과 (1)혈지:9주시HFD、HFD+ CUMS량조대서혈지이상,여혈청총담고순[(2.67±0.04)mmoL/L,(2.68 ±0.02) mmol/L]、저밀도지단백[(1.08±0.03) mmol/L,(1.06±0.01) mmol/L]명현고우CON、CON+ CUMS조[(1.78±0.12) mmol/L,(0.79±0.04) mmol/L;(1.76±0.09) mmol/L,(0.76±0.06) mmol/L,균P＜0.01],형성전형고지혈증모형.(2)open-field실험:13주시,CON+CUMS조대서여CON조대서상비,전자제1분종내운동격자적총수목증다,5 min내운동적중간격자수목、후지직립차수균감소,차명현증가수식차수급대편립수(균P＜0.05);HFD조대서부재5 min내운동총격자、중간급주변격자수목여CON조대서유차이(균P＜0.05).HFD+ CUMS조대서13주시해실험소유지표균교기선、9주현저감소(균P ＜0.001).13주시,HFD +CUMS조대서5 min내운동총격자、중간격자급주변격자수목비기타대조조대서균감소,후지직립차수감소,제1분종내운동격자적총수목、수식차수、대편립수명현증가(균P ＜0.001).결론 고지혈증미가공제기출지상,일단접촉만성응격인자회출현엄중억욱증상,병반명현자주신경공능문란증상.
Objective To investigate behavioral changes in a rat hyperlipidemia model induced by high lipid feed combined with depression by Chronic Unpredictable Mild Stress (CUMS).Methods A total of 40 rats were randomly divided into control (CON),control feed for 9 weeks followed by CUMS for 4 weeks (CON + CUMS),high fat diet (HFD) and high lipid feed for 9 weeks followed by CUMS for 4 weeks (HFD + CUMS) (n =10 each).Open-field test was individually measured at baseline,week 9 and week 13.Results (1) Serum lipids:total cholesterol [(2.67 ± 0.04) mmol/L,(2.68 ± 0.02) mmol/L] and low density lipoprotein [(1.08 ± 0.03) mmol/L,(1.06 ± 0.01) mmol/L] of HFD and HFD + CUMS were both significantly higher than those of CON and CON + CUMS [(1.78 ± 0.12) mmol/L,(0.79 ± 0.04) mmol/L;(1.76 ±0.09)mmol/L,(0.76 ±0.06)mmol/L,all P ＜0.01].(2) Open-field test:at week 13,compared to CON rats,CON + CUMS rats exhibited enhanced locomotor activity during the first minute,reduced activity in the center squares and rearing,and increased the number of grooming and defecation (all P ＜ 0.05).In comparison to the CON rats,a decrease in total squares in 5 min,central squares and peripheral squares was observed in HFD rats at week 13 (all P ＜ 0.05).However,compared with HFD,CON,CON + CUMS rats,when high lipid feed for 9 weeks combined with depression,significant decrease activities in total squares in 5 min,central squares and peripheral squares were observed in HFD + CUMS rats at week 13.Besides these,the number of rearing was reduced,however,locomotor activity during the first minute and the number of grooming and defecation was significantly increased (all P ＜ 0.001).Conclusion Under uncontrolled hyperlipidemia,severe depressive symptoms will present more early once exposure to a series of chronic stressors followed by significant autonomic nervous dysfunctional symptoms.