CAJ | 학술논문

目的探讨重楼皂苷Ⅶ联合二氧化硅纳米复合体对于卵巢癌的耐药性.方法应用MTT法测定重楼皂苷Ⅶ联合二氧化硅纳米复合体对人卵巢癌耐药细胞SKOV3/DDP的增殖抑制率;流式细胞术和Hoechst 33342染色法检测重楼皂苷Ⅶ联合二氧化硅纳米复合体对人卵巢癌耐药细胞SKOV3/DDP的细胞凋亡率及细胞周期时相分布的影响;蛋白免疫印迹法检测凋亡相关蛋白的表达改变.结果重楼皂苷Ⅶ对SKOV3/DDP细胞的增殖有明显抑制作用,抑制率随药物浓度增加而增高,具有明显的量-效关系(P＜0.05).重楼皂苷Ⅶ、二氧化硅纳米复合体对SKOV3/DDP细胞作用48、24 h组比较细胞增殖抑制率均明显提高(P＜0.05).Hoechst 33342染色结果显示,50及100μmol/L的重楼皂苷Ⅶ作用SKOV3/DDP细胞24 h后,不同剂量均能引起细胞出现凋亡的典型表现,即细胞核的深染、凝集和固缩.与阴性对照组比较早期凋亡细胞比例明显增高.蛋白灰度分析结果,与对照组比24 h重楼皂苷Ⅶ处理SKOV3/DDP细胞,50和100 μmol/L剂量组组裂解caspase 3分别增高了5.71倍和11.06倍,Bcl-2表达分别下降了33.3％和61.1％,差异有统计学意义(P＜0.05).结论重楼皂苷Ⅶ、二氧化硅纳米复合体对SKOV3/DDP增殖有明显抑制作用,呈明显的浓度依赖性,与时间有相关性,它可引起早期凋亡SKOV3/DDP细胞比例显著增加.
목적 탐토중루조감Ⅶ연합이양화규납미복합체대우란소암적내약성.방법 응용MTT법측정중루조감Ⅶ연합이양화규납미복합체대인란소암내약세포SKOV3/DDP적증식억제솔;류식세포술화Hoechst 33342염색법검측중루조감Ⅶ연합이양화규납미복합체대인란소암내약세포SKOV3/DDP적세포조망솔급세포주기시상분포적영향;단백면역인적법검측조망상관단백적표체개변.결과 중루조감Ⅶ대SKOV3/DDP세포적증식유명현억제작용,억제솔수약물농도증가이증고,구유명현적량-효관계(P＜0.05).중루조감Ⅶ、이양화규납미복합체대SKOV3/DDP세포작용48、24 h조비교세포증식억제솔균명현제고(P＜0.05).Hoechst 33342염색결과현시,50급100μmol/L적중루조감Ⅶ작용SKOV3/DDP세포24 h후,불동제량균능인기세포출현조망적전형표현,즉세포핵적심염、응집화고축.여음성대조조비교조기조망세포비례명현증고.단백회도분석결과,여대조조비24 h중루조감Ⅶ처리SKOV3/DDP세포,50화100 μmol/L제량조조렬해caspase 3분별증고료5.71배화11.06배,Bcl-2표체분별하강료33.3％화61.1％,차이유통계학의의(P＜0.05).결론 중루조감Ⅶ、이양화규납미복합체대SKOV3/DDP증식유명현억제작용,정명현적농도의뢰성,여시간유상관성,타가인기조기조망SKOV3/DDP세포비례현저증가.
Objective To explore the drug resistance of Chonglou saponin Ⅶ plus silica nano complex for ovarian cancer.Methods The method of thiazolyl blue tetrazolium blue (MTT) was employed for determining the proliferation inhibition rate of Chonglou saponin Ⅶ plus silica nano composite for SKOV3/DDP drug-resistant human ovarian cancer cell.Flow cytometry and Hoechst 33342 staining were used for detecting apoptosis rate and cell cycle phase distribution.And the changes of apoptosis-related protein were examined by immunoblot assay.Results There were obvious inhibitory effects of Chonglou saponin Ⅶ on cell proliferation.The inhibition rate increased with rising drug concentration with an obvious dose-effect relationship (P ＜ 0.05).At the same time,the cell proliferation inhibition rate significantly improved at 48 h versus 24 h (P ＜ 0.05).Flow cytometry and Hoechst 33342 staining showed that,after a 24 h treatment of SKOV3/DDP cells with 50 and 100 μmol/L Chonglou saponin Ⅶ,early apoptotic cell ratio significantly increased compared with control group.Conclusion The composite of Paris polyphylla saponin Ⅶ and silica nano has obvious proliferation inhibitory effects on SKOV3/DDP cells.And there is a significant concentration dependence and it also has some correlation with time.The above composite can increased the proportion of early apoptotic cell ratio.