中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
23期
1862-1865
,共4页
乳腺癌%淋巴结转移%裸鼠
乳腺癌%淋巴結轉移%裸鼠
유선암%림파결전이%라서
Breast neoplasms%Lymphatic metastasis%Nude mice
目的 用MDA-MB-231人乳腺癌细胞悬液及移植瘤组织块分别建立裸鼠乳腺癌淋巴转移模型.方法 雌性BALB/C裸鼠12只,5周龄,随机分成A、B两组,A组为细胞悬液组,共7只裸鼠,B组为移植瘤组织块组,共5只裸鼠.将MDA-MB-231细胞悬液各0.1 ml分别注射于A组裸鼠的右侧第2对乳腺脂肪垫下,成瘤2周后,切取2只裸鼠的原位肿瘤种植于B组裸鼠的右侧第2对乳腺脂肪垫下,A组其余5只裸鼠继续饲养.A、B两组均于接种后第6周处死,解剖裸鼠后切取原位肿瘤及腋窝肿大淋巴结进行病理检查.结果 ①A组裸鼠种植后第7天,成瘤率为7/7只,B组裸鼠种植后第5天,成瘤率为5/5只;②两组裸鼠原位肿瘤的体积差异有统计学意义(P=0.023),移植瘤组织块组的肿瘤体积＞细胞悬液组;③解剖后,A组有3只裸鼠右侧腋窝各检出1枚肿大淋巴结,淋巴结肿大率为3/5只,B组有1只裸鼠右侧腋窝检出1枚肿大淋巴结,淋巴结肿大率为1/5只,两组裸鼠腋窝淋巴结肿大率差异无统计学意义(P=0.524);④病理学检查,两组裸鼠原位肿瘤均为浸润性导管癌,A组肿大淋巴结均为反应增生性,B组为转移性.结论 原位种植MDA-MB-231移植瘤组织块建立裸鼠乳腺癌淋巴转移模型,为研究乳腺癌淋巴转移机制提供可行工具.
目的 用MDA-MB-231人乳腺癌細胞懸液及移植瘤組織塊分彆建立裸鼠乳腺癌淋巴轉移模型.方法 雌性BALB/C裸鼠12隻,5週齡,隨機分成A、B兩組,A組為細胞懸液組,共7隻裸鼠,B組為移植瘤組織塊組,共5隻裸鼠.將MDA-MB-231細胞懸液各0.1 ml分彆註射于A組裸鼠的右側第2對乳腺脂肪墊下,成瘤2週後,切取2隻裸鼠的原位腫瘤種植于B組裸鼠的右側第2對乳腺脂肪墊下,A組其餘5隻裸鼠繼續飼養.A、B兩組均于接種後第6週處死,解剖裸鼠後切取原位腫瘤及腋窩腫大淋巴結進行病理檢查.結果 ①A組裸鼠種植後第7天,成瘤率為7/7隻,B組裸鼠種植後第5天,成瘤率為5/5隻;②兩組裸鼠原位腫瘤的體積差異有統計學意義(P=0.023),移植瘤組織塊組的腫瘤體積＞細胞懸液組;③解剖後,A組有3隻裸鼠右側腋窩各檢齣1枚腫大淋巴結,淋巴結腫大率為3/5隻,B組有1隻裸鼠右側腋窩檢齣1枚腫大淋巴結,淋巴結腫大率為1/5隻,兩組裸鼠腋窩淋巴結腫大率差異無統計學意義(P=0.524);④病理學檢查,兩組裸鼠原位腫瘤均為浸潤性導管癌,A組腫大淋巴結均為反應增生性,B組為轉移性.結論 原位種植MDA-MB-231移植瘤組織塊建立裸鼠乳腺癌淋巴轉移模型,為研究乳腺癌淋巴轉移機製提供可行工具.
목적 용MDA-MB-231인유선암세포현액급이식류조직괴분별건립라서유선암림파전이모형.방법 자성BALB/C라서12지,5주령,수궤분성A、B량조,A조위세포현액조,공7지라서,B조위이식류조직괴조,공5지라서.장MDA-MB-231세포현액각0.1 ml분별주사우A조라서적우측제2대유선지방점하,성류2주후,절취2지라서적원위종류충식우B조라서적우측제2대유선지방점하,A조기여5지라서계속사양.A、B량조균우접충후제6주처사,해부라서후절취원위종류급액와종대림파결진행병리검사.결과 ①A조라서충식후제7천,성류솔위7/7지,B조라서충식후제5천,성류솔위5/5지;②량조라서원위종류적체적차이유통계학의의(P=0.023),이식류조직괴조적종류체적＞세포현액조;③해부후,A조유3지라서우측액와각검출1매종대림파결,림파결종대솔위3/5지,B조유1지라서우측액와검출1매종대림파결,림파결종대솔위1/5지,량조라서액와림파결종대솔차이무통계학의의(P=0.524);④병이학검사,량조라서원위종류균위침윤성도관암,A조종대림파결균위반응증생성,B조위전이성.결론 원위충식MDA-MB-231이식류조직괴건립라서유선암림파전이모형,위연구유선암림파전이궤제제공가행공구.
Objective To establish lymph node metastasis of breast cancer model in nude mices using MDA-MB-231 cell lines or tumor masses.Methods Divided twelve female nude mices of five weeks into A,B groups randomly.A group had seven nude mices,B group had five nude mices.A group nude mices were injected with MDA-MB-231 cells suspension into the second right mammary fat pad.Two weeks after emerged tumors,the orthotopic tumors of two nude mices of A group were dissected and then implanted into the second right mammary fat pad of B group nude mices.The other mices of A group continued to be fed.After six weeks of inoculation,we excised the tumors and the swollen lymph nodes in right axilla of all nude mices to make pathological examination.Results ①A group have a 7/7 tumor formation rate 7 days after implanted,B group was 5/5 5 days after implanted.②The tumor volumes between the two groups had evident difference(P =0.023),and the tumor volume of B group was bigger than A group.③A group had three nude mices which had one tumid lymph node respectively,the lymph node enlargement rate was 3/5;B group only had one nude mice that had one tumid lymph node,the lymph node enlargement rate was 1/5,the lymph node enlargement rate between the two groups showed no significant difference (P =0.524).④The result of pathology in the two groups testified the tumors were invasive ductal carcinoma.The swollen lymph nodes in A group were reactive hyperplasia lymph nodes;the swollen lymph nodes in B group was metastatic lymph node.Conclusions The method of orthotopic implantation with MDA-MB-231 tumor mass to establish lymph node metastasis of breast cancer model in nude mice,can provide a useful mean to research the lymph node metastasis mechanism of breast cancer.
