CAJ | 학술논문

目的：探讨1－磷酸鞘氨醇（S1P）在骨髓间充质干细胞增殖及诱导分化为血管内皮细胞中的作用。方法采用S1P预处理原代骨髓间充质干细胞( PR－MSCs )，并评估S1P对PR－MSCs增殖和分化的影响。结果 S1P能显著促进PR－MSCs的增殖，促进PR－MSCs经血管内皮生长因子( VEGF )诱导分化为血管内皮细胞，这与经S1P处理后的PR－MSCs表达VEGF及Angiopointin－1的mRNA增多有关，同时P－ERK1/2高表达于S1P预处理后的PR－MSCs中。结论 S1P能促进PR－MSCs的增殖和分化为血管内皮细胞，并且S1P通过ERK通路影响PR－MSCs的旁分泌，这将促进PR－MSCs的临床应用以及加强对S1P作用机制的了解。
목적：탐토1－린산초안순（S1P）재골수간충질간세포증식급유도분화위혈관내피세포중적작용。방법채용S1P예처리원대골수간충질간세포( PR－MSCs )，병평고S1P대PR－MSCs증식화분화적영향。결과 S1P능현저촉진PR－MSCs적증식，촉진PR－MSCs경혈관내피생장인자( VEGF )유도분화위혈관내피세포，저여경S1P처리후적PR－MSCs표체VEGF급Angiopointin－1적mRNA증다유관，동시P－ERK1/2고표체우S1P예처리후적PR－MSCs중。결론 S1P능촉진PR－MSCs적증식화분화위혈관내피세포，병차S1P통과ERK통로영향PR－MSCs적방분비，저장촉진PR－MSCs적림상응용이급가강대S1P작용궤제적료해。
Objective To investigate the effects of sphingosine-1-phosphate ( S1P ) on the proliferation and differentiation of bone mar-row mesenchymal stem cells ( MSCs ) into vascular endothelial cells. Methods Primary bone marrow MSCs ( PR-MSCs ) were pre-treat-ed with S1P and the effects of S1P on the proliferation and differentiation of PR-MSCs was evaluated. Results The proliferation ca-pability of PR-MSCs was significantly enhanced by S1P. Furthermore, S1P accelerated the differentiation of PR-MSCs into endothelial cells under vascular endothelial growth factor ( VEGF ) induction, which was related with increasing mRNA production of VEGF and An-giopointin-1 in S1P-PR-MSCs. Meanwhile, P-ERK1/2 was highly expressed in S1P treated PR-MSCs. Conclusion S1P can pro-mote the proliferation and differentiation of PR-MSCs into vascular endothelial cells. In addition, S1P can affect paracrine of PR-MSCs by activating ERK pathway, which can promote the clinical application of PR-MSCS and strengthen the understanding on SIP action mechanism.