实用医技杂志
實用醫技雜誌
실용의기잡지
JOURNAL OF PRACTICAL MEDICAL TECHNIQUES
2015年
6期
573-575
,共3页
雷公藤内酯%糖尿病心肌病%大鼠%葡萄糖转运体1型%葡萄糖转运体4型
雷公籐內酯%糖尿病心肌病%大鼠%葡萄糖轉運體1型%葡萄糖轉運體4型
뢰공등내지%당뇨병심기병%대서%포도당전운체1형%포도당전운체4형
Triptolide%Diabetic cardiomyopathies%Rats%Glucose transporter type-1%Glucose transporter type-4
目的：探讨雷公藤内酯醇对糖尿病大鼠心肌内葡萄糖转运体（Glut）-1、Glut-4表达的干预。方法雄性SD大鼠60只，按体质量随机分为正常对照组10只和糖尿病模型组50只。糖尿病模型组一次性腹腔注射链脲佐菌素53 mg/kg，正常对照组仅腹腔注射等体积0.1 mol/L柠檬酸缓冲液。注射链脲佐菌素72 h后，尾静脉采血测空腹血糖并同时测尿糖，血糖≥16.7 mmol/L、尿糖（+++）以上者列入糖尿病观察对象。将造模成功的48只雄性大鼠，按体质量、空腹血糖随机分为糖尿病模型对照组（12只），雷公藤内酯醇小、中、大剂量组（各12只）。结果雷公藤内酯醇中、大剂量组大鼠心体比值均比糖尿病对照组降低，但都高于正常对照组；雷公藤内酯醇中、大剂量组大鼠心肌中Glut-1、Glut-4的表达均比糖尿病对照组有显著增高，但都较正常对照组明显减少；雷公藤内酯醇小剂量组糖尿病大鼠心体比值、心肌中Glut-1、Glut-4的表达与糖尿病对照组相比变化不明显。结论雷公藤内酯醇能明显上调糖尿病大鼠心肌细胞内Glut-1、Glut-4的表达，改善高血糖对糖尿病大鼠心肌的毒性作用。
目的：探討雷公籐內酯醇對糖尿病大鼠心肌內葡萄糖轉運體（Glut）-1、Glut-4錶達的榦預。方法雄性SD大鼠60隻，按體質量隨機分為正常對照組10隻和糖尿病模型組50隻。糖尿病模型組一次性腹腔註射鏈脲佐菌素53 mg/kg，正常對照組僅腹腔註射等體積0.1 mol/L檸檬痠緩遲液。註射鏈脲佐菌素72 h後，尾靜脈採血測空腹血糖併同時測尿糖，血糖≥16.7 mmol/L、尿糖（+++）以上者列入糖尿病觀察對象。將造模成功的48隻雄性大鼠，按體質量、空腹血糖隨機分為糖尿病模型對照組（12隻），雷公籐內酯醇小、中、大劑量組（各12隻）。結果雷公籐內酯醇中、大劑量組大鼠心體比值均比糖尿病對照組降低，但都高于正常對照組；雷公籐內酯醇中、大劑量組大鼠心肌中Glut-1、Glut-4的錶達均比糖尿病對照組有顯著增高，但都較正常對照組明顯減少；雷公籐內酯醇小劑量組糖尿病大鼠心體比值、心肌中Glut-1、Glut-4的錶達與糖尿病對照組相比變化不明顯。結論雷公籐內酯醇能明顯上調糖尿病大鼠心肌細胞內Glut-1、Glut-4的錶達，改善高血糖對糖尿病大鼠心肌的毒性作用。
목적：탐토뢰공등내지순대당뇨병대서심기내포도당전운체（Glut）-1、Glut-4표체적간예。방법웅성SD대서60지，안체질량수궤분위정상대조조10지화당뇨병모형조50지。당뇨병모형조일차성복강주사련뇨좌균소53 mg/kg，정상대조조부복강주사등체적0.1 mol/L저몽산완충액。주사련뇨좌균소72 h후，미정맥채혈측공복혈당병동시측뇨당，혈당≥16.7 mmol/L、뇨당（+++）이상자렬입당뇨병관찰대상。장조모성공적48지웅성대서，안체질량、공복혈당수궤분위당뇨병모형대조조（12지），뢰공등내지순소、중、대제량조（각12지）。결과뢰공등내지순중、대제량조대서심체비치균비당뇨병대조조강저，단도고우정상대조조；뢰공등내지순중、대제량조대서심기중Glut-1、Glut-4적표체균비당뇨병대조조유현저증고，단도교정상대조조명현감소；뢰공등내지순소제량조당뇨병대서심체비치、심기중Glut-1、Glut-4적표체여당뇨병대조조상비변화불명현。결론뢰공등내지순능명현상조당뇨병대서심기세포내Glut-1、Glut-4적표체，개선고혈당대당뇨병대서심기적독성작용。
Objective To discuss intervention of triptolide on glucose transporter type (Glut)-1, Glut-4 expres-sion in the myocardium of diabetic rats. Methods Sixty SD rats were randomly divided into normal control group (n=10) and diabetes model (DM)group (n=50). Diabetic model group were injected of streptozotocin (STZ) 53 mg/kg intraperitoneally, the normal control group were injected volume of the buffer. After the injection of STZ 72 h , fasting glucose and simultaneously measuring urine were tested from tail vein blood , both blood glucose≥16.7 mmol/L and rather than urine (+++) were included in the DM observed object. The successful model of 48 male rats , according to body weight, fasting blood glucose, DM model were randomly divided into a control group (12), triptolide (TP) of small, medium and large-dose group (12 each). Results TP in medium (1.8 g/kg) and high dose (2.2 g/kg) of rats heart body ratio were lower than DM group significantly, but were higher than the normal control group; the expression of TP in medium and high dosage groups in myocardium of rats with Glut-1 and Glut-4 were significantly higher than DM group. But all decreased significantly comparing with normal group; TP group of small dose of diabetic rat heart body ratio, myocardial Glut-1, Glut-4 expression comparing with the DM group did not change significantly. Conclusion Triptolide significantly increases Glut-1 and Glut-4 expression in diabetic rat myocardial cells, improves the toxic effect of hyper-glycemia on myocardium of DM rats.
