广东林业科技
廣東林業科技
엄동임업과기
FORESTRY SCIENCE AND TECHNOLOGY OF GUANGDONG PROVINCE
2015年
2期
1-6
,共6页
周丽华%蓝燕群%何波祥%蔡燕灵%连辉明%张谦
週麗華%藍燕群%何波祥%蔡燕靈%連輝明%張謙
주려화%람연군%하파상%채연령%련휘명%장겸
木荷%优良家系%组织培养
木荷%優良傢繫%組織培養
목하%우량가계%조직배양
Schima superba%superior family%tissue culture
以新选育的木荷（Schima superba）优良家系为试材，开展组织培养。结果表明，木荷适宜的初代培养基为GD+6-BA 1.50 mg·L-1+NAA 0.50 mg·L-1，继代培养基为GD+6-BA 1.00 mg·L-1+ IAA 0.30mg·L-1，生根培养基为1/4 MS+ IBA 1.00 mg·L-1+IAA 0.40 mg·L-1。芽苗增殖倍数为3.1倍，生根率达96.67％。探索出适宜的驯化、移栽和后期管理技术，使优良家系生根苗的移栽存活率达到92.2％。
以新選育的木荷（Schima superba）優良傢繫為試材，開展組織培養。結果錶明，木荷適宜的初代培養基為GD+6-BA 1.50 mg·L-1+NAA 0.50 mg·L-1，繼代培養基為GD+6-BA 1.00 mg·L-1+ IAA 0.30mg·L-1，生根培養基為1/4 MS+ IBA 1.00 mg·L-1+IAA 0.40 mg·L-1。芽苗增殖倍數為3.1倍，生根率達96.67％。探索齣適宜的馴化、移栽和後期管理技術，使優良傢繫生根苗的移栽存活率達到92.2％。
이신선육적목하（Schima superba）우량가계위시재，개전조직배양。결과표명，목하괄의적초대배양기위GD+6-BA 1.50 mg·L-1+NAA 0.50 mg·L-1，계대배양기위GD+6-BA 1.00 mg·L-1+ IAA 0.30mg·L-1，생근배양기위1/4 MS+ IBA 1.00 mg·L-1+IAA 0.40 mg·L-1。아묘증식배수위3.1배，생근솔체96.67％。탐색출괄의적순화、이재화후기관리기술，사우량가계생근묘적이재존활솔체도92.2％。
A newly-selected superior family ofSchima superba was used for tissue culture and micropropagation. The explants of stem segments displayed the optimum initial growth on the primary media GD+6-BA 1.50 mg·L-1+NAA 0.50 mg·L-1, the highest proliferation rate of 3.1 times on subculture GD+6-BA 1.00 mg·L-1+IAA 0.30 mg·L-1, and 96.67% rooting rate on medium 1/4 MS+ IBA 1.00 mg·L-1+IAA0.40 mg·L-1. Suitable techniques and procedures were identified for acclimatization, transplantation and later-period management of tissue-cultured plantlets. The survival rate of the superior family was 92.2%.
