CAJ | 학술논문

目的研究两种细胞支架对骨髓间充质干细胞生物学的影响,为进一步应用于肩袖损伤的修复提供依据.方法分别用冷冻干燥法及溶剂蒸发法处理壳聚糖-β-甘油磷酸钠-胶原水凝胶,制备两种固体支架;观察两种支架表层结构的差异,测定两种支架吸水溶胀率及材料拉伸度;将兔骨髓间充质干细胞分别与两种支架组的浸提液共同培养,MTT试验分析浸提液对培养细胞的影响;支架与细胞共培养后,HE及Hoechst染色测定细胞的增殖情况,并对细胞分化方向进行检测.结果冷冻干燥组较溶剂蒸发组表现出多孔状,有较强的吸水能力和较低的拉伸机械强度;两组支架均表现出低细胞毒性,可提供骨髓间充质干细胞黏附、增殖的场所.两组支架均无明显诱导骨髓间充质干细胞向成骨细胞表型表达,溶剂蒸发组支架可以诱导其向成软骨方向分化.结论两种方法制备的支架表现出不同的性能,溶剂蒸发组支架可作为细胞载体将来有希望用于肩袖损伤中腱骨界面的修复.
목적 연구량충세포지가대골수간충질간세포생물학적영향,위진일보응용우견수손상적수복제공의거.방법 분별용냉동간조법급용제증발법처리각취당-β-감유린산납-효원수응효,제비량충고체지가;관찰량충지가표층결구적차이,측정량충지가흡수용창솔급재료랍신도;장토골수간충질간세포분별여량충지가조적침제액공동배양,MTT시험분석침제액대배양세포적영향;지가여세포공배양후,HE급Hoechst염색측정세포적증식정황,병대세포분화방향진행검측.결과 냉동간조조교용제증발조표현출다공상,유교강적흡수능력화교저적랍신궤계강도;량조지가균표현출저세포독성,가제공골수간충질간세포점부、증식적장소.량조지가균무명현유도골수간충질간세포향성골세포표형표체,용제증발조지가가이유도기향성연골방향분화.결론 량충방법제비적지가표현출불동적성능,용제증발조지가가작위세포재체장래유희망용우견수손상중건골계면적수복.
Objective To investigate the influences of two types of scaffolds on the biological behavior of bone marrow mesenchymal stem cells (BMSCs) and provide the scientific basis for tissue engineering of rotator cuff repair.Methods Two types of scaffolds were fabricated from chitosan-β-glycerophosphate-collagen hydrogel by freeze drying and solvent drying technique,respectively.The differences in surface structures between the two scaffolds were observed.The swelling ratios as well as tensile strength of the materials were tested.Rabbit BMSCs were cultured with the extracts from the two scaffolds.MTT assay was performed to evaluate cell proliferation.BMSCs were also cultured on the scaffolds.HE staining and Hoechst staining were conducted to measure cell proliferation and differentiation.Results The freeze drying scaffolds were more porous while the solvent drying scaffolds were more compact.The freeze drying scaffolds had higher swelling ratio and lower mechanical strength than the solvent drying scaffolds.Both scaffolds showed low cytotoxicity,allowed good cell attachment and proliferation of BMSCs.While differentiating of BMSCs into osteoblasts was not observed in either group,the solvent drying scaffolds did induced differentiation of BMSCs into chondrocytes.Conclusion Scaffolds prepared from two fabrication processes have different characteristics.The solvent drying scaffolds are suitable cell carriers for promoting tendon-on-bone healing in rotator cuff repair.