药学与临床研究
藥學與臨床研究
약학여림상연구
PHARMACEUTICAL AND CLINICAL RESEARCH
2015年
3期
223-225
,共3页
吡非尼酮%HepG2细胞%细胞凋亡
吡非尼酮%HepG2細胞%細胞凋亡
필비니동%HepG2세포%세포조망
Pirfenidone%HepG2 cells%Apoptosis
目的:研究吡非尼酮(pirfenidone,PF)对人肝癌细胞系HepG2增殖和凋亡的影响。方法:CCK-8法测定不同浓度PF对HepG2细胞增殖活性的影响;Hoechst 33258荧光染色法观察PF处理后HepG2细胞形态的变化;流式细胞仪检测细胞凋亡率。结果:PF对HepG2细胞具有显著增殖抑制作用,并呈浓度和时间依赖性;Hoechst 33258染色可见PF处理后细胞出现典型的凋亡形态学变化;流式细胞仪检测结果显示,与空白组比较,PF处理后的HepG2细胞凋亡率显著增加(P﹤0.01)。结论:PF对人肝癌细胞系HepG2细胞增殖具有抑制作用,且与诱导HepG2细胞凋亡有关。
目的:研究吡非尼酮(pirfenidone,PF)對人肝癌細胞繫HepG2增殖和凋亡的影響。方法:CCK-8法測定不同濃度PF對HepG2細胞增殖活性的影響;Hoechst 33258熒光染色法觀察PF處理後HepG2細胞形態的變化;流式細胞儀檢測細胞凋亡率。結果:PF對HepG2細胞具有顯著增殖抑製作用,併呈濃度和時間依賴性;Hoechst 33258染色可見PF處理後細胞齣現典型的凋亡形態學變化;流式細胞儀檢測結果顯示,與空白組比較,PF處理後的HepG2細胞凋亡率顯著增加(P﹤0.01)。結論:PF對人肝癌細胞繫HepG2細胞增殖具有抑製作用,且與誘導HepG2細胞凋亡有關。
목적:연구필비니동(pirfenidone,PF)대인간암세포계HepG2증식화조망적영향。방법:CCK-8법측정불동농도PF대HepG2세포증식활성적영향;Hoechst 33258형광염색법관찰PF처리후HepG2세포형태적변화;류식세포의검측세포조망솔。결과:PF대HepG2세포구유현저증식억제작용,병정농도화시간의뢰성;Hoechst 33258염색가견PF처리후세포출현전형적조망형태학변화;류식세포의검측결과현시,여공백조비교,PF처리후적HepG2세포조망솔현저증가(P﹤0.01)。결론:PF대인간암세포계HepG2세포증식구유억제작용,차여유도HepG2세포조망유관。
Objective: To investigate the effect of pirfenidone (PF) on cell proliferation and apoptosis of HepG2 cells in vitro. Methods: The cell proliferation inhibition of HepG2 cells by PF was observed by CCK-8 assay. The morphology of HepG2 cells with Hoechst 33258 staining was observed under a fluores-cent microscope. The apoptosis was analyzed by flow cytometry. Results: PF obviously inhibited the prolif-eration of HepG2 cells in a time and dose dependent manner. Hoechst 33258 staining showed apoptosis was induced after PF treatment. Flow cytometry results showed that PF could induce HepG2 cells apopto-sis, compared with the control group (P<0.01). Conclusion: PF inhibits the proliferation of HepG2 cells probably because of inducing HepG2 cells apoptosis.