药学与临床研究
藥學與臨床研究
약학여림상연구
PHARMACEUTICAL AND CLINICAL RESEARCH
2015年
3期
223-225
,共3页
吡非尼酮%HepG2细胞%细胞凋亡
吡非尼酮%HepG2細胞%細胞凋亡
필비니동%HepG2세포%세포조망
Pirfenidone%HepG2 cells%Apoptosis
目的：研究吡非尼酮（pirfenidone，PF）对人肝癌细胞系HepG2增殖和凋亡的影响。方法：CCK-8法测定不同浓度PF对HepG2细胞增殖活性的影响；Hoechst 33258荧光染色法观察PF处理后HepG2细胞形态的变化；流式细胞仪检测细胞凋亡率。结果：PF对HepG2细胞具有显著增殖抑制作用，并呈浓度和时间依赖性；Hoechst 33258染色可见PF处理后细胞出现典型的凋亡形态学变化；流式细胞仪检测结果显示，与空白组比较，PF处理后的HepG2细胞凋亡率显著增加（P﹤0.01）。结论：PF对人肝癌细胞系HepG2细胞增殖具有抑制作用，且与诱导HepG2细胞凋亡有关。
目的：研究吡非尼酮（pirfenidone，PF）對人肝癌細胞繫HepG2增殖和凋亡的影響。方法：CCK-8法測定不同濃度PF對HepG2細胞增殖活性的影響；Hoechst 33258熒光染色法觀察PF處理後HepG2細胞形態的變化；流式細胞儀檢測細胞凋亡率。結果：PF對HepG2細胞具有顯著增殖抑製作用，併呈濃度和時間依賴性；Hoechst 33258染色可見PF處理後細胞齣現典型的凋亡形態學變化；流式細胞儀檢測結果顯示，與空白組比較，PF處理後的HepG2細胞凋亡率顯著增加（P﹤0.01）。結論：PF對人肝癌細胞繫HepG2細胞增殖具有抑製作用，且與誘導HepG2細胞凋亡有關。
목적：연구필비니동（pirfenidone，PF）대인간암세포계HepG2증식화조망적영향。방법：CCK-8법측정불동농도PF대HepG2세포증식활성적영향；Hoechst 33258형광염색법관찰PF처리후HepG2세포형태적변화；류식세포의검측세포조망솔。결과：PF대HepG2세포구유현저증식억제작용，병정농도화시간의뢰성；Hoechst 33258염색가견PF처리후세포출현전형적조망형태학변화；류식세포의검측결과현시，여공백조비교，PF처리후적HepG2세포조망솔현저증가（P﹤0.01）。결론：PF대인간암세포계HepG2세포증식구유억제작용，차여유도HepG2세포조망유관。
Objective: To investigate the effect of pirfenidone (PF) on cell proliferation and apoptosis of HepG2 cells in vitro. Methods: The cell proliferation inhibition of HepG2 cells by PF was observed by CCK-8 assay. The morphology of HepG2 cells with Hoechst 33258 staining was observed under a fluores-cent microscope. The apoptosis was analyzed by flow cytometry. Results: PF obviously inhibited the prolif-eration of HepG2 cells in a time and dose dependent manner. Hoechst 33258 staining showed apoptosis was induced after PF treatment. Flow cytometry results showed that PF could induce HepG2 cells apopto-sis, compared with the control group (P<0.01). Conclusion: PF inhibits the proliferation of HepG2 cells probably because of inducing HepG2 cells apoptosis.
