CAJ | 학술논문

薄层色谱法分析糖类混合物的组分简便快速，但是并不能将甘露糖、阿拉伯糖、半乳糖、木糖、葡萄糖进行彻底分离。本文采用双向层析的方法分别以V乙腈∶V水＝85∶15为第一向展层剂，以V正丁醇∶V乙酸乙酯∶V水＝7∶2∶1为第二向展层剂优化层析。结果显示，得到了较好的分离效果，可以定性检测出糖类组分。对比国内外研究，此方法简单，因此双向薄层色谱法可以作为一种简单、准确、清晰、便利的方法定性检测糖类混合物的组分。
박층색보법분석당류혼합물적조분간편쾌속，단시병불능장감로당、아랍백당、반유당、목당、포도당진행철저분리。본문채용쌍향층석적방법분별이V을정∶V수＝85∶15위제일향전층제，이V정정순∶V을산을지∶V수＝7∶2∶1위제이향전층제우화층석。결과현시，득도료교호적분리효과，가이정성검측출당류조분。대비국내외연구，차방법간단，인차쌍향박층색보법가이작위일충간단、준학、청석、편리적방법정성검측당류혼합물적조분。
The thin layer chromatography ( TLC) analysis of the mixed saccharides liquid composition effect is good , simple and rapid , but it doesn′t put the mannose , arabinose , galactose , xylose , glucose separately .This paper using the two step-TLC, the first step by Vacetonitrile∶Vwater =85∶15,and the second step by Vn-butanol∶V ethyl acetate∶V Water =7∶2∶1 as developing solvent for optimization of chromatography .Results show that using the above method to analyze saccharides mixture , got the good separation effect , can the qualitative detection of saccharides components , comparative research both at home and abroad , the use effect of the method is very simple and intuitive.Therefore, the two step thin layer chromatography ( TLC) can be used as a simple , accurate, clear and convenient method of qualitative detection of saccharide mixture components .