CAJ | 학술논문

目的：构建聚合物／液晶细胞模型，探究其表面弹性对大鼠骨髓干细胞黏附情况的影响。方法：溶剂挥发致相分离方法构建聚合物／液晶细胞模型，通过偏光显微镜、扫描式电子显微镜及X射线衍射观察细胞模型的表面特征。全骨髓细胞贴壁培养法分离大鼠骨髓间充质干细胞（ BMSCs），通过表面因子检测和诱导其成骨成脂分化进行鉴定。培养3代后分4组：纯PU空白对照组，10％膜组，30％膜组和50％膜组。接种细胞密度为5×107／L，待细胞贴壁24h后作细胞骨架染色处理，激光共聚焦显微镜观察细胞附着在材料上的情况并拍照。结果：溶剂挥发致相分离方法成功构建3种不同硬度的聚合物／液晶细胞模型；表面因子检测显示CD29、CD44、CD90呈阳性表达，而CD34和CD45呈阴性表达；经过茜素红S染色和油红O染色，成骨成脂分化形成的钙结节和脂滴都显而易见；细胞骨架染色拍摄结果显示，纯PU对照组、10％膜组和30％膜组上的细胞铺展面积较广，肌动蛋白微丝和细胞核清晰明显；而50％膜组的细胞铺展面积较小，细胞核虽较清晰，但是肌动蛋白微丝却模糊不清。结论：合适液晶含量的聚合物／液晶生物膜能支持大鼠骨髓干细胞的贴壁生长，而液晶含量过高则抑制细胞的贴壁生长。
목적：구건취합물／액정세포모형，탐구기표면탄성대대서골수간세포점부정황적영향。방법：용제휘발치상분리방법구건취합물／액정세포모형，통과편광현미경、소묘식전자현미경급X사선연사관찰세포모형적표면특정。전골수세포첩벽배양법분리대서골수간충질간세포（ BMSCs），통과표면인자검측화유도기성골성지분화진행감정。배양3대후분4조：순PU공백대조조，10％막조，30％막조화50％막조。접충세포밀도위5×107／L，대세포첩벽24h후작세포골가염색처리，격광공취초현미경관찰세포부착재재료상적정황병박조。결과：용제휘발치상분리방법성공구건3충불동경도적취합물／액정세포모형；표면인자검측현시CD29、CD44、CD90정양성표체，이CD34화CD45정음성표체；경과천소홍S염색화유홍O염색，성골성지분화형성적개결절화지적도현이역견；세포골가염색박섭결과현시，순PU대조조、10％막조화30％막조상적세포포전면적교엄，기동단백미사화세포핵청석명현；이50％막조적세포포전면적교소，세포핵수교청석，단시기동단백미사각모호불청。결론：합괄액정함량적취합물／액정생물막능지지대서골수간세포적첩벽생장，이액정함량과고칙억제세포적첩벽생장。
[ ABSTRACT] AIM:To develop the cell model of polymer/liquid crystal and to study the effect of their elasticity on the adhesion of rat bone marrow mesenchymal stem cells (rBM-MSCs).METHODS: Using the method of solvent e-vaporation induced phase separation, the cell model of polymer/liquid crystal was constructed.The surface morphology and phase separation structure were determined by polarized optical microscopy ( POM) , scanning electron microscopy ( SEM) and small angle X-ray scattering ( SAXS ) .rBM-MSCs were separated and expanded by adherent culture.The surface markers of rBM-MSCs were detected by flow cytometry.The cells were induced to osteogenic differentiation and adipogenic differentiation for 2 weeks.After 3 passages, the cells were divided into 4 groups, including total PU control group, 10%membrane group, 30%membrane group and 50%membrane group.The cells were then incubated with rhodamine phalloi-din for cytoskeleton staining and were observed under the confocal laser scanning microscope after cultured for 24 h.RE-SULTS:The cell model of polymer/liquid crystal was constructed successfully using the method of solvent evaporation in-duced phase separation.Flow cytometry results showed that the rBM-MSCs positively expressed CD29, CD44 and CD90, and negatively expressed CD34 and CD45.After stained with alizarin red S and oil red O, the calcium nodule and lipid droplets in rBM-MSCs were observed obviously.The cytoskeleton staining result indicated that the area in total PU control group, 10%membrane group and 30%membrane group were greater, and the actin microfilaments were also clearer than <br> that in 50%membrane group.CONCLUSION:The cell model with suitable content of liquid crystal made a contribution to the rBM-MSCs’ adhesion, but too much liquid crystal inhibits cell adhesion.