CAJ | 학술논문

目的：观察诱导性多能干细胞来源的间充质干细胞（iPSC－MSCs）对氯化钴（CoCl2）诱导的PC12细胞损伤的影响，并探讨其可能机制。方法：用CoCl2处理PC12细胞建立化学损伤模型，加入iPSC－MSCs共培养。用细胞计数试剂盒－8（CCK－8）比色法检测细胞存活率，Annexin V／PI双染流式细胞术检测细胞凋亡比率，JC－1染色流式细胞术检测细胞线粒体膜电位，免疫荧光观察iPSC－MSCs向PC12细胞转移线粒体的情况。结果：用CoCl2（400μmol／L）处理PC12细胞24 h可使其凋亡明显增多，线粒体膜电位明显下降。与iPSC－MSCs共培养能减轻PC12细胞的凋亡，使其膜电位恢复。 iPSC－MSCs可以与PC12细胞间形成隧道纳米管并向PC12细胞转移线粒体。结论：iPSC－MSCs可以减轻CoCl2诱导的PC12细胞损伤，机制可能与其向PC12细胞转移线粒体有关。
목적：관찰유도성다능간세포래원적간충질간세포（iPSC－MSCs）대록화고（CoCl2）유도적PC12세포손상적영향，병탐토기가능궤제。방법：용CoCl2처리PC12세포건립화학손상모형，가입iPSC－MSCs공배양。용세포계수시제합－8（CCK－8）비색법검측세포존활솔，Annexin V／PI쌍염류식세포술검측세포조망비솔，JC－1염색류식세포술검측세포선립체막전위，면역형광관찰iPSC－MSCs향PC12세포전이선립체적정황。결과：용CoCl2（400μmol／L）처리PC12세포24 h가사기조망명현증다，선립체막전위명현하강。여iPSC－MSCs공배양능감경PC12세포적조망，사기막전위회복。 iPSC－MSCs가이여PC12세포간형성수도납미관병향PC12세포전이선립체。결론：iPSC－MSCs가이감경CoCl2유도적PC12세포손상，궤제가능여기향PC12세포전이선립체유관。
[ ABSTRACT] AIM:To investigate the effects of induced pluripotent stem cells-derived mesenchymal stem cells ( iPSC-MSCs) on cobalt chloride ( CoCl2 )-induced injuries of PC12 cells and its possible mechanism.METHODS:PC12 cells were exposed to CoCl2 to set up a chemical-induced cellular injury model and were cocultured with iPSC-MSCs.The cell viability was tested by CCK-8 assay.The apoptosis was measured by flow cytometry using Annexin V/PI staining.The mitochondrial membrane potential (MMP) was analyzed by flow cytometry using JC-1 staining.Immunofluorescence was employed to observe mitochondrial transfer from iPSC-MSCs to PC12 cells.RESULTS: Apoptosis of PC12 cells was in-creased and MMP of PC12 cells was decreased after exposed to CoCl2 at concentration of 400μmol/L for 24 h.Coculture of PC12 cells with iPSC-MSCs reduced the apoptosis and recovered the MMP of the PC12 cells.Tunneling nanotubes were formed between iPSC-MSCs and PC12 cells, through which the iPSC-MSCs transferred the mitochondria to the PC12 cells. CONCLUSION:iPSC-MSCs protect PC12 cells from CoCl2-induced injuries, which may be associated with the mitochon-drial transfer from iPSC-MSCs to PC12 cells.