中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2015年
6期
1032-1035
,共4页
刘洪涛%周凡涵%申媛媛%张艾洁%赖艳梅%厉红元
劉洪濤%週凡涵%申媛媛%張艾潔%賴豔梅%厲紅元
류홍도%주범함%신원원%장애길%뢰염매%려홍원
WNT5B表达%乳腺癌
WNT5B錶達%乳腺癌
WNT5B표체%유선암
WNT5B expression%Breast cancer
目的：研究WNT5B在乳腺癌中的表达，初步探讨WNT5B与乳腺癌临床病理特征的关系。方法：采用real－time PCR和Western blot法检测67例乳腺癌及癌旁组织中WNT5B在mRNA和蛋白水平的表达情况，通过免疫组织化学方法检测WNT5B在乳腺癌和癌旁组织中的表达并分析WNT5B的表达与相关临床病理指标之间的关系。结果：67例乳腺癌组织标本中均有不同程度WNT5B的mRNA及蛋白表达，且WNT5B在乳腺癌组织的表达明显低于癌旁组织（P＜0．05）；其中WNT5B在原发性肿瘤大小T≤20 mm的乳腺癌患者中的表达明显高于其在T＞20 mm组患者中的表达（P＜0．05）；WNT5B的表达与乳腺癌患者腋窝淋巴结转移情况、组织学分级、相关的免疫组织化学指标（ ER、PR、c－ErBb－2、p53和Ki－67）之间无明显的相关性（ P＞0．05）。结论： WNT5 B在乳腺癌组织中的表达低于癌旁组织，WNT5B表达与乳腺癌患者原发肿瘤大小呈负相关，提示WNT5B可能作为乳腺癌预后的一个新的分子标志物，为乳腺癌的诊治提供新的思路。
目的：研究WNT5B在乳腺癌中的錶達，初步探討WNT5B與乳腺癌臨床病理特徵的關繫。方法：採用real－time PCR和Western blot法檢測67例乳腺癌及癌徬組織中WNT5B在mRNA和蛋白水平的錶達情況，通過免疫組織化學方法檢測WNT5B在乳腺癌和癌徬組織中的錶達併分析WNT5B的錶達與相關臨床病理指標之間的關繫。結果：67例乳腺癌組織標本中均有不同程度WNT5B的mRNA及蛋白錶達，且WNT5B在乳腺癌組織的錶達明顯低于癌徬組織（P＜0．05）；其中WNT5B在原髮性腫瘤大小T≤20 mm的乳腺癌患者中的錶達明顯高于其在T＞20 mm組患者中的錶達（P＜0．05）；WNT5B的錶達與乳腺癌患者腋窩淋巴結轉移情況、組織學分級、相關的免疫組織化學指標（ ER、PR、c－ErBb－2、p53和Ki－67）之間無明顯的相關性（ P＞0．05）。結論： WNT5 B在乳腺癌組織中的錶達低于癌徬組織，WNT5B錶達與乳腺癌患者原髮腫瘤大小呈負相關，提示WNT5B可能作為乳腺癌預後的一箇新的分子標誌物，為乳腺癌的診治提供新的思路。
목적：연구WNT5B재유선암중적표체，초보탐토WNT5B여유선암림상병리특정적관계。방법：채용real－time PCR화Western blot법검측67례유선암급암방조직중WNT5B재mRNA화단백수평적표체정황，통과면역조직화학방법검측WNT5B재유선암화암방조직중적표체병분석WNT5B적표체여상관림상병리지표지간적관계。결과：67례유선암조직표본중균유불동정도WNT5B적mRNA급단백표체，차WNT5B재유선암조직적표체명현저우암방조직（P＜0．05）；기중WNT5B재원발성종류대소T≤20 mm적유선암환자중적표체명현고우기재T＞20 mm조환자중적표체（P＜0．05）；WNT5B적표체여유선암환자액와림파결전이정황、조직학분급、상관적면역조직화학지표（ ER、PR、c－ErBb－2、p53화Ki－67）지간무명현적상관성（ P＞0．05）。결론： WNT5 B재유선암조직중적표체저우암방조직，WNT5B표체여유선암환자원발종류대소정부상관，제시WNT5B가능작위유선암예후적일개신적분자표지물，위유선암적진치제공신적사로。
[ ABSTRACT] AIM:To study the expression of WNT5B in the breast cancer and further to discuss the correlation between WNT5B and clinicopathologic characteristics of breast cancer.METHODS:The expression of WNT5B at mRNA and protein levels was measured by real-time PCR and Western blot in 67 cases of breast cancer and the tissue adjacent to carcinoma.In addition, the immunohistochemical method was used to detect the expression of WNT5B in the breast cancer and the tissue adjacent to carcinoma.The relationships between WNT5B expression and clinicopathologic indexes were also analyzed.RESULTS:The expression of WNT5B in the breast cancer was obviously lower than that in the tissue adjacent to carcinoma (P<0.05).The expression of WNT5B at mRNA and protein levels in 67 samples of breast cancer was in va-rious degrees.The expression of WNT5B in T≤20 mm group of human breast cancer was obviously higher than that in T>20 mm group (P<0.05).The expression of WNT5B had no obvious correlation with axillary lymph node metastasis, histo-logical grade and immunohistochemical indexes of ER, PR, c-ErBb-2, p53 and Ki67 ( P>0.05) in the breast cancer. CONCLUSION:The expression of WNT5B decreases obviously in breast cancer.The expression of WNT5B is related to primary tumor size, which provides new ideas for the diagnosis and treatment of breast cancer, suggesting that WNT5B may be a new molecular marker for prognosis of breast cancer.
