CAJ | 학술논문

目的：探讨纳米金（gold nanoparticles，GNPs）对人肝癌阿霉素（adriamycin，ADM）耐药细胞株HepG2／ADM的阿霉素耐药性的逆转作用及其可能机制。方法：MTT比色法检测GNPs对人肝癌细胞株HepG2及其耐药细胞株HepG2／ADM对不同浓度 ADM 耐药性的影响；流式细胞术检测 GNPs 处理后 ADM （2 mg／L ）对HepG2／ADM细胞凋亡的影响；紫外分光光度计检测GNPs处理后HepG2／ADM细胞内ADM的药物浓度；谷胱甘肽（ GSH）检测试剂盒（ DTNB法）检测GNPs处理后HepG2／ADM细胞GSH的含量。结果：GNPs处理前HepG2和HepG2／ADM细胞对不同浓度ADM的半数抑制浓度分别为：（9．16±2．03）mg／L、（29．46±1．73）mg／L，耐药倍数为3．22；GNPs处理后HepG2／ADM 细胞对不同浓度ADM的半数抑制浓度为（15．18±0．85）mg／L，逆转指数为1．95。GNPs＋ADM组HepG2／ADM的细胞凋亡率明显高于单独ADM组（P＜0．05）。 HepG2／ADM组细胞内的ADM含量低于HepG2组细胞内的ADM含量（ P＜0．01）；GNPs处理后HepG2／ADM细胞内的ADM含量较处理前明显增加（P＜0．01）。 HepG2／ADM组细胞内GSH含量高于HepG2组（P＜0．01）；GNPs处理后HepG2／ADM细胞内的GSH含量较处理前明显降低（ P＜0．05）。结论：纳米金具有逆转耐药肝癌细胞对阿霉素耐药性的作用，并且可以降低细胞内GSH含量及增加胞内化疗药物浓度。
목적：탐토납미금（gold nanoparticles，GNPs）대인간암아매소（adriamycin，ADM）내약세포주HepG2／ADM적아매소내약성적역전작용급기가능궤제。방법：MTT비색법검측GNPs대인간암세포주HepG2급기내약세포주HepG2／ADM대불동농도 ADM 내약성적영향；류식세포술검측 GNPs 처리후 ADM （2 mg／L ）대HepG2／ADM세포조망적영향；자외분광광도계검측GNPs처리후HepG2／ADM세포내ADM적약물농도；곡광감태（ GSH）검측시제합（ DTNB법）검측GNPs처리후HepG2／ADM세포GSH적함량。결과：GNPs처리전HepG2화HepG2／ADM세포대불동농도ADM적반수억제농도분별위：（9．16±2．03）mg／L、（29．46±1．73）mg／L，내약배수위3．22；GNPs처리후HepG2／ADM 세포대불동농도ADM적반수억제농도위（15．18±0．85）mg／L，역전지수위1．95。GNPs＋ADM조HepG2／ADM적세포조망솔명현고우단독ADM조（P＜0．05）。 HepG2／ADM조세포내적ADM함량저우HepG2조세포내적ADM함량（ P＜0．01）；GNPs처리후HepG2／ADM세포내적ADM함량교처리전명현증가（P＜0．01）。 HepG2／ADM조세포내GSH함량고우HepG2조（P＜0．01）；GNPs처리후HepG2／ADM세포내적GSH함량교처리전명현강저（ P＜0．05）。결론：납미금구유역전내약간암세포대아매소내약성적작용，병차가이강저세포내GSH함량급증가포내화료약물농도。
AIM:To investigate whether gold nanoparticles (GNPs) reverses adriamycin (ADM), resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM and to explore the potential mechanism.METH-ODS:The sensitivities of HepG2 cells and HepG2/ADM cells to ADM were tested by MTT assay before and after GNPs pretreatment.The apoptotic rate was examined by flow cytometry.The concentration of ADM in HepG2/ADM or HepG2 cells was determined by ultraviolet-visible spectrophotometer .The content of glutathione ( GSH ) in HepG2/ADM or HepG2 cells by DTNB method.RESULTS:The half maximal inhibitory concentrations ( IC50 ) of ADM for HepG2/ADM cells were(29.46 ±1.73) mg/L and (15.18 ±0.85) mg/L before and after GNPs pretreatment,respectively.The IC50 of ADM for HepG2 cells was (9.16 ±2.03) mg/L before pretreatment.The apoptotic rate in GNPs+ADM group was higher than that in ADM group ( P<0.05 ) .The concentration of ADM in HepG2/ADM group was lower than that in HepG2 group (P<0.01).After GNPs pretreatment, the concentration of ADM in HepG2/ADM cells was higher than that before pretreatment.The content of GSH in HepG2/ADM group was higher than that in HepG2 group (P<0.01).After GNPs pretreatment, the content of GSH in the HepG2/ADM cells was lower than that before pretreatment.CONCLUSION:Gold nanoparticles can reverse ADM resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM, <br> reduce the content of GSH and increase the concentration of ADM in HepG2/ADM cells.