中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2015年
6期
973-979
,共7页
有氧运动%心力衰竭%交感神经%自主神经功能
有氧運動%心力衰竭%交感神經%自主神經功能
유양운동%심력쇠갈%교감신경%자주신경공능
KEY WORDS] Aerobic exercise%Heart failure%Sympathetic nerve%Autonomic nervous function
目的:探讨长期有氧运动对心梗后心力衰竭(心衰)大鼠模型左心室及交感神经重塑(结构重塑与功能重塑)的影响,为心衰的机制研究及康复治疗提供科学依据和有效方法。方法:健康雄性Wistar大鼠通过结扎冠状动脉前降支建立心梗后心衰模型,术后4周随机分为假手术安静组(S组)、心衰安静组(H组)和心衰运动组( HE组)。 HE组进行10周跑台训练,S组和H组保持安静状态。超声心动术检测心脏结构与功能,即左室舒张期内径( LVIDd)、左室收缩期内径( LVIDs)、左室舒张期前壁厚度( LVAWDd)、左室收缩期前壁厚度( LVAWDs)、左室舒张期后壁厚度(LVPWDd)、左室收缩期后壁厚度(LVPWDs)、缩短分数(FS)和左室射血分数(LVEF);Masson染色进行心脏组织病理学观察并获得心肌胶原容积分数( CVF );高压液相色谱法检测心肌和血浆去甲肾上腺素( NE)水平;经皮下引导电极连续采集心电信号,对自主神经功能参数———心率变异性( HRV)进行频域分析,包括总功率谱( TP)、归一化低频功率谱( LFn)、归一化高频功率谱( HFn)和LF/HF比值;实时荧光定量PCR检测心肌I型胶原( Col-I)、III型胶原( Col-III)、心房钠尿因子( ANF)、α-肌球蛋白重链(α-MHC)、β-肌球蛋白重链(β-MHC)和肌质网Ca2+-ATP酶( SERCA2a) mRNA表达,Western blotting法检测心肌神经生长因子( NGF)及其受体( TrkA)和酪氨酸羟化酶( TH)蛋白表达。结果:(1)与S组比较,H组体重( BW)、LVIDd、FS、LVEF、TP、HFn、α-MHC和SER-CA2a的mRNA,NGF、TrkA和TH的蛋白表达降低( P<0.05);左室重量( LVW)、左室质量指数( LVMI)、LVAWDd、LVAWDs、LVPWDd、LVPWDs、CVF、血浆和心肌NE含量、LFn、LF/HF、ANF、β-MHC、Col-I和Col-III的 mRNA表达升高(P<0.05)。(2)与H组比较,HE组LVW、LVMI、LVIDd、FS、LVEF、TP、HFn、α-MHC和SERCA2a的 mRNA, NGF、TrkA和TH的蛋白表达升高(P<0.05);CVF、血浆和心肌NE含量、LFn、LF/HF、ANF、β-MHC、Col-I和Col-III的mRNA表达降低(P<0.05)。结论:长期有氧运动可抑制心梗后心衰大鼠左室重塑与交感神经重塑,心功能和自主调节改善。
目的:探討長期有氧運動對心梗後心力衰竭(心衰)大鼠模型左心室及交感神經重塑(結構重塑與功能重塑)的影響,為心衰的機製研究及康複治療提供科學依據和有效方法。方法:健康雄性Wistar大鼠通過結扎冠狀動脈前降支建立心梗後心衰模型,術後4週隨機分為假手術安靜組(S組)、心衰安靜組(H組)和心衰運動組( HE組)。 HE組進行10週跑檯訓練,S組和H組保持安靜狀態。超聲心動術檢測心髒結構與功能,即左室舒張期內徑( LVIDd)、左室收縮期內徑( LVIDs)、左室舒張期前壁厚度( LVAWDd)、左室收縮期前壁厚度( LVAWDs)、左室舒張期後壁厚度(LVPWDd)、左室收縮期後壁厚度(LVPWDs)、縮短分數(FS)和左室射血分數(LVEF);Masson染色進行心髒組織病理學觀察併穫得心肌膠原容積分數( CVF );高壓液相色譜法檢測心肌和血漿去甲腎上腺素( NE)水平;經皮下引導電極連續採集心電信號,對自主神經功能參數———心率變異性( HRV)進行頻域分析,包括總功率譜( TP)、歸一化低頻功率譜( LFn)、歸一化高頻功率譜( HFn)和LF/HF比值;實時熒光定量PCR檢測心肌I型膠原( Col-I)、III型膠原( Col-III)、心房鈉尿因子( ANF)、α-肌毬蛋白重鏈(α-MHC)、β-肌毬蛋白重鏈(β-MHC)和肌質網Ca2+-ATP酶( SERCA2a) mRNA錶達,Western blotting法檢測心肌神經生長因子( NGF)及其受體( TrkA)和酪氨痠羥化酶( TH)蛋白錶達。結果:(1)與S組比較,H組體重( BW)、LVIDd、FS、LVEF、TP、HFn、α-MHC和SER-CA2a的mRNA,NGF、TrkA和TH的蛋白錶達降低( P<0.05);左室重量( LVW)、左室質量指數( LVMI)、LVAWDd、LVAWDs、LVPWDd、LVPWDs、CVF、血漿和心肌NE含量、LFn、LF/HF、ANF、β-MHC、Col-I和Col-III的 mRNA錶達升高(P<0.05)。(2)與H組比較,HE組LVW、LVMI、LVIDd、FS、LVEF、TP、HFn、α-MHC和SERCA2a的 mRNA, NGF、TrkA和TH的蛋白錶達升高(P<0.05);CVF、血漿和心肌NE含量、LFn、LF/HF、ANF、β-MHC、Col-I和Col-III的mRNA錶達降低(P<0.05)。結論:長期有氧運動可抑製心梗後心衰大鼠左室重塑與交感神經重塑,心功能和自主調節改善。
목적:탐토장기유양운동대심경후심력쇠갈(심쇠)대서모형좌심실급교감신경중소(결구중소여공능중소)적영향,위심쇠적궤제연구급강복치료제공과학의거화유효방법。방법:건강웅성Wistar대서통과결찰관상동맥전강지건립심경후심쇠모형,술후4주수궤분위가수술안정조(S조)、심쇠안정조(H조)화심쇠운동조( HE조)。 HE조진행10주포태훈련,S조화H조보지안정상태。초성심동술검측심장결구여공능,즉좌실서장기내경( LVIDd)、좌실수축기내경( LVIDs)、좌실서장기전벽후도( LVAWDd)、좌실수축기전벽후도( LVAWDs)、좌실서장기후벽후도(LVPWDd)、좌실수축기후벽후도(LVPWDs)、축단분수(FS)화좌실사혈분수(LVEF);Masson염색진행심장조직병이학관찰병획득심기효원용적분수( CVF );고압액상색보법검측심기화혈장거갑신상선소( NE)수평;경피하인도전겁련속채집심전신호,대자주신경공능삼수———심솔변이성( HRV)진행빈역분석,포괄총공솔보( TP)、귀일화저빈공솔보( LFn)、귀일화고빈공솔보( HFn)화LF/HF비치;실시형광정량PCR검측심기I형효원( Col-I)、III형효원( Col-III)、심방납뇨인자( ANF)、α-기구단백중련(α-MHC)、β-기구단백중련(β-MHC)화기질망Ca2+-ATP매( SERCA2a) mRNA표체,Western blotting법검측심기신경생장인자( NGF)급기수체( TrkA)화락안산간화매( TH)단백표체。결과:(1)여S조비교,H조체중( BW)、LVIDd、FS、LVEF、TP、HFn、α-MHC화SER-CA2a적mRNA,NGF、TrkA화TH적단백표체강저( P<0.05);좌실중량( LVW)、좌실질량지수( LVMI)、LVAWDd、LVAWDs、LVPWDd、LVPWDs、CVF、혈장화심기NE함량、LFn、LF/HF、ANF、β-MHC、Col-I화Col-III적 mRNA표체승고(P<0.05)。(2)여H조비교,HE조LVW、LVMI、LVIDd、FS、LVEF、TP、HFn、α-MHC화SERCA2a적 mRNA, NGF、TrkA화TH적단백표체승고(P<0.05);CVF、혈장화심기NE함량、LFn、LF/HF、ANF、β-MHC、Col-I화Col-III적mRNA표체강저(P<0.05)。결론:장기유양운동가억제심경후심쇠대서좌실중소여교감신경중소,심공능화자주조절개선。
[ ABSTRACT] AIM:To investigate the effects of long-term aerobic exercise on the heart and sympathetic neural remodeling ( structure and function remodeling) in heart failure rats induced by myocardial infarction.METHODS:Heart failure model after myocardial infarction was performed by ligating anterior descending coronary artery in the Wistar rats. Four weeks after operation, the rats were randomly divided into sham operation sedentary ( S) group, heart failure sedenta-ry ( H) group and heart failure exercise ( HE) group.The animals in HE group underwent 10-week treadmill running, while those in S group and H group were sustained in a resting state.The cardiac structure and function including left ven-tricular internal diameter at diastole ( LVIDd) , left ventricular internal diameter at systole ( LVIDs) , left ventricular anteri- <br> or wall diameter at diastole (LVAWDd), left ventricular anterior wall diameter at systole (LVAWDs), left ventricular pos-terior wall diameter at diastole ( LVPWDd) and left ventricular posterior wall diameter at systole ( LVPWDs) , and cardiac function parameters including fractional shortening (FS) and left ventricular ejection fraction (LVEF) were measured by echocardiography.The myocardium was collected for histopathological observation with Masson staining, and the collagen volume fraction ( CVF) was determined.The concentrations of norepinephrine ( NE) in the myocardium and plasma were measured by high-pressure liquid chromatography.The frequency domain analysis was applied for determining the heart rate variability (HRV) via subcutaneous recording electrode involving total power (TP), normalized low power (LFn), nor-malized high power ( HFn) and LF/HF ratio.The mRNA expression of collagen type I ( Col-I) , collagen type III ( Col-III) , atrial natriuretic factor ( ANF) ,α-myosin heavy chain (α-MHC) ,β-myosin heavy chain (β-MHC) , sarcoplasmic endoplasmic reticulum Ca2+-ATPase (SERCA2a) was detected by real-time PCR.The protein levels of nerve growth factor (NGF) and its receptor (TrkA), and tyrosine hydroxylase (TH) were measured by Western blotting.RESULTS:(1) Compared with S group, body weight ( BW) , LVIDd, FS, LVEF, TP, HFn, the mRNA expression ofα-MHC and SER-CA2a, and the protein levels of NGF, TrkA and TH decreased (P<0.05).Left ventricular weight (LVW), left ventricu-lar mass index (LVMI), LVAWDd, LVAWDs, LVPWDd, LVPWDs, CVF, plasma and myocardial NE content, LFn, LF/HF, and the mRNA expression of ANF,β-MHC, Col-I and Col-III increased ( P<0.05) in H group.(2) Compared with H group, LVW, LVMI, LVIDd, FS, LVEF, TP, HFn, the mRNA expression ofα-MHC and SERCA2a, and the pro-tein levels of NGF, TrkA and TH were raised (P<0.05), while CVF, plasma and myocardial NE content, LFn, LF/HF, and the mRNA expression of ANF, β-MHC, Col-I and Col-III decreased ( P<0.05 ) in HE group.CONCLUSION:Long-term aerobic exercise training leads to inhibition of heart and sympathetic neural remodeling and improvement of cardi-ac function and autonomic modulation in the rats after myocardial infarction.