CAJ | 학술논문

目的：对东方田鼠（Microtus fortis，Mf）肝内巨噬细胞进行分离纯化和功能鉴定。方法采用在体灌注、胶原酶消化及梯度密度离心相结合的方法分离Mf肝内巨噬细胞，并采用流式和墨汁吞噬功能实验进行巨噬细胞的功能鉴定。结果通过此方法获得Mf肝脏来源的巨噬细胞，分离的细胞呈透亮圆形，贴壁生长，活细胞比例为95%。Mf来源的巨噬细胞与抗鼠CD14流式单抗（Clone：Sa2?8）阳性结合率是小鼠来源巨噬细胞与该抗体结合率的50%左右。Mf肝脏来源的巨噬细胞墨汁吞噬实验阳性。结论该方法能有效地分离纯化Mf肝内巨噬细胞，可为进一步研究东方田鼠肝脏巨噬细胞抗血吸虫机制奠定基础。
목적：대동방전서（Microtus fortis，Mf）간내거서세포진행분리순화화공능감정。방법채용재체관주、효원매소화급제도밀도리심상결합적방법분리Mf간내거서세포，병채용류식화묵즙탄서공능실험진행거서세포적공능감정。결과통과차방법획득Mf간장래원적거서세포，분리적세포정투량원형，첩벽생장，활세포비례위95%。Mf래원적거서세포여항서CD14류식단항（Clone：Sa2?8）양성결합솔시소서래원거서세포여해항체결합솔적50%좌우。Mf간장래원적거서세포묵즙탄서실험양성。결론해방법능유효지분리순화Mf간내거서세포，가위진일보연구동방전서간장거서세포항혈흡충궤제전정기출。
Objective To separate and purify intrahepatic macrophages from Microtus fortis Mf and identify its phagocy?tosis. Methods The intrahepatic macrophages from Mf were separated and purified by perfusion collagenase digestion and density gradient centrifugation. The function of the cells was identified by FACS analysis and ink phagocytosis activity. Results The macrophage cells from the liver of Mf were obtained. These cells were bright and circular and grew adhering to the wall. The proportion of the living cells was 95%. The binding rate of these cells from Mf with anti?mouse CD14 antibody Clone Sa2?8 was about 50%of the rate of macrophage from C57BL/6 mice with this monoclonal antibody. The result of ink?phagocytosis ex?periment of macrophage cells from the liver of Mf was positive. Conclusion The method above mentioned is useful to separate and purify macrophage from the liver of Mf. The study builds the foundation for further research on macrophages of Mf against Schistosoma japonicum.