中国血吸虫病防治杂志
中國血吸蟲病防治雜誌
중국혈흡충병방치잡지
CHINESE JOURNAL OF SCHISTOSOMIASIS CONTROL
2015年
3期
263-267,272
,共6页
王庆权%郭见多%操治国%汪奇志%刘道华%汪天平
王慶權%郭見多%操治國%汪奇誌%劉道華%汪天平
왕경권%곽견다%조치국%왕기지%류도화%왕천평
隐孢子虫%ELISA%改良抗酸染色法%安徽省
隱孢子蟲%ELISA%改良抗痠染色法%安徽省
은포자충%ELISA%개량항산염색법%안휘성
Cryptosporidium%ELISA%Modified acid fast staining method%Anhui Province
目的:了解安徽省人群隐孢子虫感染状况,探讨感染的危险因素,为隐孢子虫病防治提供科学依据。方法选择安徽省潜山县和灵璧县为调查点,于2014年9月-2015年1月,采用改良抗酸染色法和酶联免疫吸附试验(ELISA)法,分别检查调查对象粪便中隐孢子虫卵囊和血清中隐孢子虫特异性抗体IgG,以确定隐孢子虫感染情况;同时对调查对象进行问卷调查,了解隐孢子虫感染的危险因素。结果共调查668人,其中病原学检查635人,阳性15人,阳性率为2.36%;血清学检查642人,阳性140人,阳性率为21.81%;有628人同时进行了病原学和血清学检查,两种检查方法均为阳性者12人(潜山县4人,灵璧县8人),阳性率为1.94%。潜山县与灵璧县人群隐孢子虫感染率分别为1.24%(4/322)和2.71%(8/295),差异无统计学意义(P>0.05)。单因素χ2分析发现,隐孢子虫感染率在儿童及腹泻人群较高;多因素Lo?gistics回归分析表明,隐孢子虫感染率在饲养家禽人群及腹泻人群较高。结论安徽省局部地区人群隐孢子虫血清抗体阳性率较高,提示既往感染较为严重。人群隐孢子虫感染率与低龄、腹泻和家庭饲养家禽有关,在今后防治工作中值得关注。
目的:瞭解安徽省人群隱孢子蟲感染狀況,探討感染的危險因素,為隱孢子蟲病防治提供科學依據。方法選擇安徽省潛山縣和靈璧縣為調查點,于2014年9月-2015年1月,採用改良抗痠染色法和酶聯免疫吸附試驗(ELISA)法,分彆檢查調查對象糞便中隱孢子蟲卵囊和血清中隱孢子蟲特異性抗體IgG,以確定隱孢子蟲感染情況;同時對調查對象進行問捲調查,瞭解隱孢子蟲感染的危險因素。結果共調查668人,其中病原學檢查635人,暘性15人,暘性率為2.36%;血清學檢查642人,暘性140人,暘性率為21.81%;有628人同時進行瞭病原學和血清學檢查,兩種檢查方法均為暘性者12人(潛山縣4人,靈璧縣8人),暘性率為1.94%。潛山縣與靈璧縣人群隱孢子蟲感染率分彆為1.24%(4/322)和2.71%(8/295),差異無統計學意義(P>0.05)。單因素χ2分析髮現,隱孢子蟲感染率在兒童及腹瀉人群較高;多因素Lo?gistics迴歸分析錶明,隱孢子蟲感染率在飼養傢禽人群及腹瀉人群較高。結論安徽省跼部地區人群隱孢子蟲血清抗體暘性率較高,提示既往感染較為嚴重。人群隱孢子蟲感染率與低齡、腹瀉和傢庭飼養傢禽有關,在今後防治工作中值得關註。
목적:료해안휘성인군은포자충감염상황,탐토감염적위험인소,위은포자충병방치제공과학의거。방법선택안휘성잠산현화령벽현위조사점,우2014년9월-2015년1월,채용개량항산염색법화매련면역흡부시험(ELISA)법,분별검사조사대상분편중은포자충란낭화혈청중은포자충특이성항체IgG,이학정은포자충감염정황;동시대조사대상진행문권조사,료해은포자충감염적위험인소。결과공조사668인,기중병원학검사635인,양성15인,양성솔위2.36%;혈청학검사642인,양성140인,양성솔위21.81%;유628인동시진행료병원학화혈청학검사,량충검사방법균위양성자12인(잠산현4인,령벽현8인),양성솔위1.94%。잠산현여령벽현인군은포자충감염솔분별위1.24%(4/322)화2.71%(8/295),차이무통계학의의(P>0.05)。단인소χ2분석발현,은포자충감염솔재인동급복사인군교고;다인소Lo?gistics회귀분석표명,은포자충감염솔재사양가금인군급복사인군교고。결론안휘성국부지구인군은포자충혈청항체양성솔교고,제시기왕감염교위엄중。인군은포자충감염솔여저령、복사화가정사양가금유관,재금후방치공작중치득관주。
Objective To investigate the status of Cryptosporidium infection in the population in the local area of Anhui Prov?ince,and discuss the risk factors of the infection,so as to provide the evidence for the prevention and treatment of cryptosporidi?osis. Methods Qianshan County and Lingbi County of Anhui Province were selected as investigation spots,and the oocysts of Cryptosporidium in the feces of the investigation objects and the specific IgG antibody against Cryptosporidium in the serum were checked by using the pathogenic modified acid fast staining method and ELISA,respectively,so as to determine the status of Cryptosporidium infection in these investigation objects. At the same time,the questionnaire surveys were conducted in the inves?tigation objects so as to know about the risk factors of Cryptosporidium infection. Results A total of 668 people were investigat?ed in the two counties,635 people received etiological examinations,and 15 people were positive with the positive rate of 2.36%;642 people received serological examinations,and 140 people were positive with the positive rate of 21.81%;628 peo?ple received pathogenic and serological examinations at the same time,and the examination results of the both methods showed that 12 people were positive(there were 4 people in Qianshan County and 8 people in Lingbi County),and the positive rate was 1.94%. The rates of Cryptosporidium infection in the population of Qianshan County and Lingbi County were 1.24%(4/322)and 2.71%(8/295)respectively,and the difference had no statistical significance(P>0.05). The single factor analysis found that the rate of Cryptosporidium infection was higher in the children and diarrhea patients;the multivariate logistics regression analy?sis indicated that the rate of Cryptosporidium infection was higher in the people who bred poultry and the diarrhea patients. Con?clusions The positive rate of serum antibody of Cryptosporidium in the population of the local area of Anhui Province is higher, which indicates that the previous infection is serious,and the rate of Cryptosporidium infection in human is relative to the age, diarrhea and whether there are poultries to be bred in the family,which is worthy of attention in the future prevention and treat?ment.