现代检验医学杂志
現代檢驗醫學雜誌
현대검험의학잡지
JOURNAL OF MODERN LABORATORY MEDICINE
2015年
3期
83-86
,共4页
王虹%李丽丽%张吉才%高波%骆海军
王虹%李麗麗%張吉纔%高波%駱海軍
왕홍%리려려%장길재%고파%락해군
甲基化%耐药%hMLH1%MGMT%5-氮杂-2′脱氧胞苷
甲基化%耐藥%hMLH1%MGMT%5-氮雜-2′脫氧胞苷
갑기화%내약%hMLH1%MGMT%5-담잡-2′탈양포감
methylation%drug fast%hMLH1%MGMT%5-Aza-Cde
目的：观察5-氮杂-2′脱氧胞苷（5-Aza-Cde）对体外培养的顺铂（DDP）耐药株肺癌 A549／DDP 细胞 hMLH1，MG-MT 基因启动子区 DNA 甲基化状态及其表达的影响，探讨肺癌细胞 hMLH1和 MGMT 基因失活的机制及去甲基化制剂对 hMLH1和 MGMT 基因表达的调控。方法5-Aza-Cde 处理体外1640培养的肺癌 A549／DDP 细胞，甲基化特异性PCR（MSP）法检测用药前后细胞 hMLH1和 MGMT 基因的甲基化状态，RT-PCR 法检测用药前后细胞 hMLH1和 MG-MT mRNA 的表达。结果在对照组 A549细胞当中 hMLH1基因是非甲基化状态和高表达，而 MGMT 显示为低甲基化（部分甲基化）状态和高表达；而在顺铂耐药株 A549-DDP 中，hMLH1和 MGMT 基因均显示高甲基化状态，mRNA 表达下调。结论hMLH1和 MGMT 基因甲基化修饰程度与 mRNA 的表达呈相关性改变，证明 hMLH1，MGMT 基因甲基化可能是 A549对顺铂耐药的因素之一。
目的：觀察5-氮雜-2′脫氧胞苷（5-Aza-Cde）對體外培養的順鉑（DDP）耐藥株肺癌 A549／DDP 細胞 hMLH1，MG-MT 基因啟動子區 DNA 甲基化狀態及其錶達的影響，探討肺癌細胞 hMLH1和 MGMT 基因失活的機製及去甲基化製劑對 hMLH1和 MGMT 基因錶達的調控。方法5-Aza-Cde 處理體外1640培養的肺癌 A549／DDP 細胞，甲基化特異性PCR（MSP）法檢測用藥前後細胞 hMLH1和 MGMT 基因的甲基化狀態，RT-PCR 法檢測用藥前後細胞 hMLH1和 MG-MT mRNA 的錶達。結果在對照組 A549細胞噹中 hMLH1基因是非甲基化狀態和高錶達，而 MGMT 顯示為低甲基化（部分甲基化）狀態和高錶達；而在順鉑耐藥株 A549-DDP 中，hMLH1和 MGMT 基因均顯示高甲基化狀態，mRNA 錶達下調。結論hMLH1和 MGMT 基因甲基化脩飾程度與 mRNA 的錶達呈相關性改變，證明 hMLH1，MGMT 基因甲基化可能是 A549對順鉑耐藥的因素之一。
목적：관찰5-담잡-2′탈양포감（5-Aza-Cde）대체외배양적순박（DDP）내약주폐암 A549／DDP 세포 hMLH1，MG-MT 기인계동자구 DNA 갑기화상태급기표체적영향，탐토폐암세포 hMLH1화 MGMT 기인실활적궤제급거갑기화제제대 hMLH1화 MGMT 기인표체적조공。방법5-Aza-Cde 처리체외1640배양적폐암 A549／DDP 세포，갑기화특이성PCR（MSP）법검측용약전후세포 hMLH1화 MGMT 기인적갑기화상태，RT-PCR 법검측용약전후세포 hMLH1화 MG-MT mRNA 적표체。결과재대조조 A549세포당중 hMLH1기인시비갑기화상태화고표체，이 MGMT 현시위저갑기화（부분갑기화）상태화고표체；이재순박내약주 A549-DDP 중，hMLH1화 MGMT 기인균현시고갑기화상태，mRNA 표체하조。결론hMLH1화 MGMT 기인갑기화수식정도여 mRNA 적표체정상관성개변，증명 hMLH1，MGMT 기인갑기화가능시 A549대순박내약적인소지일。
Objective To investigate the effects of 5-Aza-2′-deoxycytidine (5-Aza-Cde)on DNA methylation and expression of hMLH1 and MGMT gene in the human lung cancer cell line A549/DDP.Methods A549/DDP cells were cultured with RPMI 1 640 medium and were treated with 5 μmol/L DNA methyhransferase inhibitor 5-Aza-Cde.Methylation-specific pol-ymerase chain reaetioll (MSP)was used to detect the promoter methylation state of the hMLH1 and MGMT gene.RT-PCR was used to detect the mRNA expression of hMLH1 and MGMT before and after treatment with 5-Aza-Cde,respectively. Results Before treatment with 5-Aza-Cde,hMLH1 and MGMT expressions were absent,and promoter hypermethylation of the hMLH1 and MGMT gene were detected in A549 cells.After treatment with 5-Aza-Cde,the promoter region of the hM-LH1 and MGMT gene exhibited a demethylation state,and their mRNA expressions were increased.Conclusion Promoter hypermethyhtion is amajor mechanism of hMLH1 and MGMT gene silencing in human lung cancer cells,and can be reversed by the demethylating agent 5-Aza-Cde,which can regulate the expressions of the hMLH1 and MGMT gene.
