CAJ | 학술논문

目的：探讨 Reprimo 和 hMLH1基因启动子区甲基化检测在胃癌早期诊断中的价值。方法通过陕西省人民医院2013年9月～2014年4月收治的慢性萎缩性胃炎伴肠上皮化生患者50例、异型增生患者50例、胃癌患者50例，取内镜下胃活检组织，并取正常胃黏膜活检组织30例作为对照组，分析 Reprimo 基因和 hMLH1基因启动子区甲基化表达情况，比较各组患者间的差异。结果患者组的胃黏膜组织中 Reprimo 基因和 hMLH1基因启动子区甲基化阳性率显著高于正常组，差异有统计学意义（P ＜0.05）。Reprimo 基因启动子区甲基化阳性率：慢性萎缩性胃炎伴肠上皮化生组28％（χ2＝10.18，P ＜0.05）；异型增生组56％（χ2＝25.84，P ＜0.05）；胃癌组62％（χ2＝30.36，P ＜0.05）；hMLH1基因启动子区甲基化阳性率：慢性萎缩性胃炎伴肠上皮化生组20％（χ2＝4.39，P ＜0.05）；异型增生组44％（χ2＝15.13，P ＜0.05）；胃癌组48％（χ2＝17.41，P ＜0.05），检测的特异度高。结论Reprimo 和 hMLH1基因甲基化检测在胃癌早期诊断中的价值很高，特异度高，是一种有效的诊断方式，在患者的临床诊断方面拥有广阔的治疗前景。
목적：탐토 Reprimo 화 hMLH1기인계동자구갑기화검측재위암조기진단중적개치。방법통과합서성인민의원2013년9월～2014년4월수치적만성위축성위염반장상피화생환자50례、이형증생환자50례、위암환자50례，취내경하위활검조직，병취정상위점막활검조직30례작위대조조，분석 Reprimo 기인화 hMLH1기인계동자구갑기화표체정황，비교각조환자간적차이。결과환자조적위점막조직중 Reprimo 기인화 hMLH1기인계동자구갑기화양성솔현저고우정상조，차이유통계학의의（P ＜0.05）。Reprimo 기인계동자구갑기화양성솔：만성위축성위염반장상피화생조28％（χ2＝10.18，P ＜0.05）；이형증생조56％（χ2＝25.84，P ＜0.05）；위암조62％（χ2＝30.36，P ＜0.05）；hMLH1기인계동자구갑기화양성솔：만성위축성위염반장상피화생조20％（χ2＝4.39，P ＜0.05）；이형증생조44％（χ2＝15.13，P ＜0.05）；위암조48％（χ2＝17.41，P ＜0.05），검측적특이도고。결론Reprimo 화 hMLH1기인갑기화검측재위암조기진단중적개치흔고，특이도고，시일충유효적진단방식，재환자적림상진단방면옹유엄활적치료전경。
Objective To investigate the Reprimo and hMLH1 gene promoter methylation detection value in the diagnosis of early gastric cancer.Methods Chose patints in Shaanxi Provincial People’s Hospital from September 2013 to April 2014,50 cases of patients with chronic atrophicgastritis with intestinal metaplasia,50 cases of patients with gastricmucosal atypical hyperplasia,50 patients with gastric cancer,endoscopic gastric biopsy samples,and 30 cases of normal gastric mucosa biopsy tissues as control group.Analysis abnormal expression in Reprimo gene and hMLH1 genes promoter methylation,compared the differences.between groups of patients.Results The patients with gastric mucosatissues Reprimo and hMLH1 genes promoter methylation positive rate was significantly higher than that of normal group,the difference wasstatistically signifi-cant (P<0.05).Reprimo gene promoter methylation were:patients with chronic atrophic gastritis with intestinal metaplasia 28% (χ2 =10.18,P < 0.05).Patients with gastric mucosalatypical hyperplasia 56% (χ2 = 25.84,P < 0.05)and patients with gastric cancer 62% (χ2 = 30.36,P < 0.05).hMLH1 gene promoter methylation were:patients with chronic atrophic gastritis with intestinal metaplasia 20% (χ2 =4.39,P <0.05),patients with gastric mucosal atypical hyperplasia 44% (χ2 =15.13,P <0.05)and patients with gastric cancer 48% (χ2 = 17.41,P <0.05),high specificity of detection.Conclusion Reprimo and hMLH1 gene’s detect value in the diagnosis of early gastric cancer is very high,high specificity,it is an effec-tive way of diagnosis,treatment in clinical diagnosis of patients with broad prospects.