CAJ | 학술논문

目的：应用基因测序技术检测分析 UGT1A1基因启动子多态性特点，并探讨 UGT1A1*28和 UGT1A1*6基因多态性在武汉地区的分布。方法收集2013年1月～2014年12月武汉大学人民医院肿瘤内科230例肿瘤患者外周血，通过 Sanger 测序法测定靶片段的基因序列，分析患者 UGT1A1基因启动子区 TA 盒多态性。结果研究检测的230例肿瘤患者中，TA6／TA6野生型198例（86.1％），TA6／TA7杂合型29例（12.6％），TA7／TA7变异型3例（1.3％）。结论武汉地区恶性肿瘤患者 UGT1A1基因 TA6／TA6野生型最常见。
목적：응용기인측서기술검측분석 UGT1A1기인계동자다태성특점，병탐토 UGT1A1*28화 UGT1A1*6기인다태성재무한지구적분포。방법수집2013년1월～2014년12월무한대학인민의원종류내과230례종류환자외주혈，통과 Sanger 측서법측정파편단적기인서렬，분석환자 UGT1A1기인계동자구 TA 합다태성。결과연구검측적230례종류환자중，TA6／TA6야생형198례（86.1％），TA6／TA7잡합형29례（12.6％），TA7／TA7변이형3례（1.3％）。결론무한지구악성종류환자 UGT1A1기인 TA6／TA6야생형최상견。
Objective To detect UGT1A1 gene promoter polymorphism with gene sequence method and analyze the distribu-tion of UGT1A1*28 and UGT1A1*6 gene polymorphism in Wuhan.Methods A total of 230 samples were collected from tumor patient in Medical Oncology Department of Renmin Hospital of Wuhan University from January 2013 to December 2014,then gene sequence of the target fragments were detected by Sanger sequencing method and analyzed patient UGT1A1 gene promoter polymorphism TA box.Results In this research examination 230 example tumor patient,198 patients (86.1%)were identified with TA6/TA6 genotype,29 patients (12.6%)were identified with TA6/TA7 genotype,3 pa-tients (1.3%)TA7/TA7 variant.Conclusion TA6/TA6 wild-typewas higher frequence in patients with malignant tumour in Wuhan.